PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2416 [new locus tag: SAUSA300_RS13375 ]
• pan locus tag^?: SAUPAN006035000
• symbol: SAUSA300_2416
• pan gene symbol^?: —
• synonym:
• product: glucose 1-dehydrogenase-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2416 [new locus tag: SAUSA300_RS13375 ]
• symbol: SAUSA300_2416
• product: glucose 1-dehydrogenase-like protein
• replicon: chromosome
• strand: -
• coordinates: 2601933..2602652
• length: 720
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914752 NCBI
• RefSeq: YP_495051 NCBI
• BioCyc: see SAUSA300_RS13375
• MicrobesOnline: 1293931 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGGCGGTAGACATAGCTGAAGCGGTATCGGAGACTGTCGATAAAATTAAAAGTAATGGT
  GACAATGCGAAGGCGTATAATGTGGATAATGCAAGCGAACAACAAGTGGTAGACTTTGTG
  TCTGACATAAAGGAACAGTTTGGAAGAATCGATGTGTTGTTTAATAATGCCGGTGTGGAT
  AATGCGGCTGATAGAATTCATGAGTATCCAATAGATGTGTATGACAAGATTATGAATGTA
  GATATGCGTGGGACATTTTTAATGACGAAAATGATGTTACCTTTAATGATGAATCAAGGT
  GGCTCTATTGTTAATACGTCTTCATTTTCCGGACAAGCAGCAGACTTGTATCGCTCTGGA
  TATAATGCTGCGAAAGGTGCAGTGATTAATTTTACAAAATCAATCGCAATTGAGTATGGC
  CGTGATAGCATTCGAGCCAATGCGATTGCACCAGGTACAATTGAAACCCCGTTAGTAGAT
  AAACTGACAGGTACGAGTGAGGATGATGCAGGTAAAACATTTAGAGAAAATCAAAAATGG
  ATGACTCCGCTGGGACGTTTAGGTAAACCAGAAGAAGTTGCTAAATTAGTAGTCTTCTTA
  GCATCTGACGACAGTTCATTCATAACTGGAGAGACGATTCGAATTGATGGTGGTGTGATG
  GCTTACACATGGCCTGGCGAGATGTTAAGTGATGATTCGTGGAAGCGGACATTGGAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2416 [new locus tag: SAUSA300_RS13375 ]
• symbol: SAUSA300_2416
• description: glucose 1-dehydrogenase-like protein
• length: 239
• theoretical pI: 4.3468
• theoretical MW: 26189.3
• GRAVY: -0.277406

⊟Function

• reaction:
  EC 1.1.1.47?  ExPASy
  Glucose 1-dehydrogenase (NAD(P)⁺) D-glucose + NAD(P)⁺ = D-glucono-1,5-lactone + NAD(P)H
• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 166.6)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 155.3)
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 150.5)
  Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 134.9)
  2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 134.8)
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 133.8)
  and 8 more
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 127)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 126.4)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 98.4)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 92.6)
  rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 77.8)
  cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 67)
  pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 53.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-dependent protochlorophyllide reductase (TIGR01289; EC 1.3.1.33; HMM-score: 13.3)
• TheSEED  :
  • 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)
  Fatty Acids, Lipids, and Isoprenoids Fatty acids Fatty Acid Biosynthesis FASII  3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)
• PFAM:
  NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 202.7)
  and 2 more
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 135.5)
  KR; KR domain (PF08659; HMM-score: 24.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008699
  • TAT(Tat/SPI): 0.000437
  • LIPO(Sec/SPII): 0.001715
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 162138562 NCBI
• RefSeq: YP_495051 NCBI
• UniProt: A0A0H2XGP5 UniProt

⊟Protein sequence

• MAVDIAEAVSETVDKIKSNGDNAKAYNVDNASEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAADRIHEYPIDVYDKIMNVDMRGTFLMTKMMLPLMMNQGGSIVNTSSFSGQAADLYRSGYNAAKGAVINFTKSIAIEYGRDSIRANAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLGRLGKPEEVAKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications