PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2423 [new locus tag: NWMN_RS13930 ]
• pan locus tag^?: SAUPAN006174000
• symbol: NWMN_2423
• pan gene symbol^?: —
• synonym:
• product: hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2423 [new locus tag: NWMN_RS13930 ]
• symbol: NWMN_2423
• product: hydrolase
• replicon: chromosome
• strand: -
• coordinates: 2666702..2667502
• length: 801
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331496 NCBI
• RefSeq: YP_001333457 NCBI
• BioCyc:
• MicrobesOnline: 3708025 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGTTAAGGCTATTGCGGTAGATATGGACGGGACGTTTTTAGATTCAAAAAAGACATAT
  GATAAACTTAGATTTGAAGCGATTTTTACTGAACTTAGAAATAGAGATATTACATTTATT
  GCTGCGAGTGGCAATCAATATGCGAAGTTGAAGTCTATTTTCGGGGATAGAGATATGTAT
  TTTATTTCTGAAAATGGTGCAGTTATTTATAATGGCAATGAGTTATATAATTATAAAAGC
  TTTAATCGTCAGGTGTTTCAACAGGTTGTCGATTACTTAAATATGAAGCAAAGTATTGAT
  CAACTCGTCATCTGTGGTTTGAAAAGCGCGTATATTTTAAAACATACTTCTGAAGCGTTT
  AAAGAAGATACGAGATTTTATTATCATCAGTTAAAAGAAATTGACAGTCTACAGCAATTA
  CCTGAGGATGATTATGTCAAAATAGCATTTAATATTAATCGTGAGACGCATCCGAATGTT
  GACGAAGAAGTAGCAACGCAATTCAGCAATGATATTAAACTTGTCTCAAGTGGGAACGAT
  AGCATTGATATTATTATGCCAAACATGACTAAAGGGCAGGCATTAAAACGATTGTTAGAT
  AAATGGGAAATGTCTCCTTCAGAGCTCATGGCCTTCGGAGATGCGAATAATGATAAAGAT
  ATGTTAGCGTTCGCTAAGCATAGTTATGTGATGGAAAATAGTCATGATGAAGAATTGTTC
  AATATTGCCTCTGCTGTCGCTCCAAGTAATGATAAGCAGGGTGTATTAACGATTATCGAA
  CAAGAAGTGTTGAAAAAGTAG
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⊟Protein

⊟General

• locus tag: NWMN_2423 [new locus tag: NWMN_RS13930 ]
• symbol: NWMN_2423
• description: hydrolase
• length: 266
• theoretical pI: 4.75965
• theoretical MW: 30543.3
• GRAVY: -0.426692

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 164.6)
  and 15 more
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 81.6)
  sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 68.5)
  phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 63.5)
  sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 49.6)
  mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 38.5)
  mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 36.8)
  sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 35.4)
  Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 34)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 20.5)
  phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 19.4)
  Cellular processes Cellular processes Adaptations to atypical conditions trehalose-phosphatase (TIGR00685; EC 3.1.3.12; HMM-score: 16.4)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair recombination protein rad52 (TIGR00607; HMM-score: 16.2)
  Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 15.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 14.2)
  Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 12.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 169.4)
  and 3 more
  S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 69.4)
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 19.4)
  Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 16.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 0
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005216
  • TAT(Tat/SPI): 0.001023
  • LIPO(Sec/SPII): 0.000572
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222635 NCBI
• RefSeq: YP_001333457 NCBI
• UniProt: A0A0H3KAL0 UniProt

⊟Protein sequence

• MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASGNQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQLVICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNVDEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANNDKDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications