Jump to navigation
Jump to search
NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02831
- pan locus tag?: SAUPAN006174000
- symbol: SAOUHSC_02831
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02831
- symbol: SAOUHSC_02831
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2609299..2610099
- length: 801
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3921270 NCBI
- RefSeq: YP_501290 NCBI
- BioCyc: G1I0R-2667 BioCyc
- MicrobesOnline: 1291261 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGGTTAAGGCTATTGCGGTAGATATGGACGGGACGTTTTTAGATTCAAAAAAGACATAT
GATAAACTTAGATTTGAAGCGATTTTTACTGAACTTAGAAATAGAGATATTACATTTATT
GCTGCGAGTGGCAATCAATATGCGAAGTTGAAGTCTATTTTCGGGGATAGAGATATGTAT
TTTATTTCTGAAAATGGTGCAGTTATTTATAATGGCAATGAGTTATATAATTATAAAAGC
TTTAATCGTCAGGTGTTTCAACAGGTTGTCGATTACTTAAATATGAAGCAAAGTATTGAT
CAACTCGTCATCTGTGGTTTGAAAAGCGCGTATATTTTAAAACATACTTCTGAAGCGTTT
AAAGAAGATACGAGATTTTATTATCATCAGTTAAAAGAAATTGACAGTCTACAGCAATTA
CCTGAGGATGATTATGTCAAAATAGCATTTAATATTAATCGTGAGACGCATCCGAATGTT
GACGAAGAAGTAGCAACGCAATTCAGCAATGATATTAAACTTGTCTCAAGTGGGCACGAT
AGCATTGATATTATTATGCCAAACATGACTAAAGGGCAGGCATTAAAACGATTGTTAGAT
AAATGGGAAATGTCTCCTTCAGAGCTCATGGCCTTCGGAGATGCGAATAATGATAAAGAT
ATGTTAGCGTTCGCTAAGCATAGTTATGTGATGGAAAATAGTCATGATGAAGAATTGTTC
AATATTGCCTCTGCTGTCGCTCCAAGTAATGATAAGCAGGGTGTATTAACGATTATCGAA
CAAGAAGTGTTGAAAAAGTAG60
120
180
240
300
360
420
480
540
600
660
720
780
801
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02831
- symbol: SAOUHSC_02831
- description: hypothetical protein
- length: 266
- theoretical pI: 4.84613
- theoretical MW: 30566.4
- GRAVY: -0.425564
⊟Function[edit | edit source]
- TIGRFAM: Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 165.3)and 15 moreUnknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 80.4)sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 68)phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 62.6)sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 49.3)mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 37.1)mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 36.7)sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 36.7)Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 32.2)Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 20.3)phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 19.4)Cellular processes Adaptations to atypical conditions trehalose-phosphatase (TIGR00685; EC 3.1.3.12; HMM-score: 16.7)DNA metabolism DNA replication, recombination, and repair recombination protein rad52 (TIGR00607; HMM-score: 16.2)Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 15.5)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 14.2)Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 13.1)
- TheSEED :
- Hydrolase (HAD superfamily)
- PFAM: HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 169.8)and 2 moreS6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 69.7)Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 19.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 0
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005216
- TAT(Tat/SPI): 0.001023
- LIPO(Sec/SPII): 0.000572
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASGNQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQLVICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNVDEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANNDKDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)