From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2462 [new locus tag: NWMN_RS14145 ]
  • pan locus tag?: SAUPAN006226000
  • symbol: crtM
  • pan gene symbol?: crtM
  • synonym:
  • product: squalene desaturase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2462 [new locus tag: NWMN_RS14145 ]
  • symbol: crtM
  • product: squalene desaturase
  • replicon: chromosome
  • strand: -
  • coordinates: 2709770..2710633
  • length: 864
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACAATGATGGATATGAATTTTAAATATTGTCATAAAATCATGAAGAAACATTCAAAA
    AGCTTTTCTTACGCTTTTGACTTGTTACCAGAAGATCAAAGAAAAGCGGTTTGGGCAATT
    TATGCTGTGTGTCGTAAAATTGATGACAGTATAGATGTTTATGGCGATATTCAATTTTTA
    AATCAAATAAAAGAAGATATACAATCTATTGAAAAATACCCATATGAACATCATCACTTT
    CAAAGTGATCGTAGAATCATGATGGCGCTTCAGCATGTTGCACAACATAAAAATATCGCC
    TTTCAATCTTTTTATAATCTCATTGATACTGTATATAAAGATCAACATTTTACAATGTTT
    GAAACGGACGCTGAATTATTCGGATATTGTTATGGTGTTGCTGGTACAGTAGGTGAAGTA
    TTGACGCCGATTTTAAGTGATCATGAAACACATCAGACATACGATGTCGCAAGAAGACTT
    GGTGAATCGTTGCAATTGATTAATATATTAAGAGATGTCGGTGAAGATTTTGACAATGAA
    CGGATATATTTTAGTAAGCAACGATTAAAGCAATATGAAGTTGATATTGCTGAAGTGTAC
    CAAAATGGTGTTAATAATCATTATATTGACTTATGGGAATATTATGCAGCTATCGCAGAA
    AAAGATTTTCAAGATGTTATGGATCAAATCAAAGTATTTAGTATTGAAGCACAACCAATC
    ATAGAATTAGCAGCACGTATATATATTGAAATACTGGACGAAGTGAGACAGGCTAACTAT
    ACATTACATGAACGTGTTTTTGTGGATAAGAGGAAAAAGGCAAAGTTGTTTCATGAAATA
    AATAGTAAATATCATAGAATATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    864

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2462 [new locus tag: NWMN_RS14145 ]
  • symbol: CrtM
  • description: squalene desaturase
  • length: 287
  • theoretical pI: 6.01098
  • theoretical MW: 34230.7
  • GRAVY: -0.449477

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.96?  ExPASy
    4,4'-diapophytoene synthase 2 (2E,6E)-farnesyl diphosphate = 15-cis-4,4'-diapophytoene + 2 diphosphate
  • TIGRFAM:
    squalene synthase HpnD (TIGR03465; EC 2.5.1.21; HMM-score: 144.9)
    and 2 more
    squalene synthase HpnC (TIGR03464; EC 2.4.1.21; HMM-score: 101.2)
    farnesyl-diphosphate farnesyltransferase (TIGR01559; EC 2.5.1.21; HMM-score: 51.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Terp_synthase (CL0613) SQS_PSY; Squalene/phytoene synthase (PF00494; HMM-score: 195.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003464
    • TAT(Tat/SPI): 0.000176
    • LIPO(Sec/SPII): 0.000456
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVCRKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQSFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRLGESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAIAEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKLFHEINSKYHRI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1] [2]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  2. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_2462&oldid=71837"