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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2510 [new locus tag: NWMN_RS14400 ]
- pan locus tag?: SAUPAN006314000
- symbol: NWMN_2510
- pan gene symbol?: betB
- synonym:
- product: glycine betaine aldehyde dehydrogenase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2510 [new locus tag: NWMN_RS14400 ]
- symbol: NWMN_2510
- product: glycine betaine aldehyde dehydrogenase
- replicon: chromosome
- strand: -
- coordinates: 2757380..2758870
- length: 1491
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331561 NCBI
- RefSeq: YP_001333544 NCBI
- BioCyc:
- MicrobesOnline: 3708112 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441ATGGAACTTTTAAAACATTTATCTCAGCGTCAATATATTGATGGTGAGTGGGTTGAAAGC
GCGAATAAAAATACAAGAGATATTATCAATCCTTACAATCAAGAAGTGATATTTACGGTT
TCTGAAGGGACAAAAGAGGATGCAGAACGTGCAATCTTAGCTGCAAGACGTGCGTTTGAG
TCTGGTGAATGGTCGCAAGAAACTGCTGAAACAAGAGGTAAAAAAGTACGTGCCATCGCG
GACAAGATTAAAGAACATCGCGAAGCGTTAGCACGATTAGAAACATTAGATACTGGAAAA
ACGTTAGAAGAATCATATGCAGATATGGATGATATTCATAATGTGTTTATGTATTTTGCT
GGATTAGCAGATAAAGACGGTGGCGAAATGATTGATTCACCAATTCCAGATACAGAAAGC
AAAATTGTTAAAGAACCAGTAGGTGTAGTTACACAAATTACACCTTGGAATTATCCGTTA
TTACAAGCATCATGGAAAATTGCGCCAGCGCTTGCTACGGGTTGTTCACTAGTTATGAAA
CCAAGTGAAATTACACCATTAACAACAATACGTGTTTTTGAATTAATGGAAGAAGTTGGT
TTCCCTAAAGGAACAATTAATCTTATTCTAGGTGCAGGTTCTGAAGTTGGTGACGTAATG
TCAGGTCATAAAGAGGTTGACCTTGTATCATTTACAGGTGGCATTGAGACTGGTAAGCAT
ATTATGAAAAATGCTGCTAATAATGTTACGAATATTGCCTTGGAACTTGGCGGTAAAAAT
CCAAACATTATCTTTGATGATGCTGATTTTGAATTGGCAGTAGACCAAGCGTTAAATGGT
GGATATTTCCATGCAGGTCAAGTTTGTTCAGCAGGATCAAGAATATTAGTACAAAACAGT
ATTAAAGACAAATTTGAGCAAGCACTTATTGATCGCGTGAAAAAAATCAAATTAGGTAAT
GGTTTTGATGCTGATACTGAAATGGGACCAGTGATTTCAACAGAACATCGTAATAAGATC
GAATCTTATATGGATGTAGCTAAAGCAGAAGGCGCAACAATTGCTGTTGGTGGTAAACGT
CCAGATAGAGATGATTTAAAAGATGGTCTATTCTTCGAGCCAACAGTCATTACAAATTGT
GATACGTCAATGCGTATTGTACAAGAAGAGGTTTTCGGACCTGTCGTTACTGTAGAAGGC
TTTGAAACTGAACAAGAAGCGATTCAATTAGCGAATGATTCTATATATGGTTTAGCAGGT
GCTGTATTTTCTAAAGATATTGGAAAAGCACAACGCGTTGCTAACAAGTTGAAACTTGGA
ACGGTGTGGATTAATGATTTCCATCCATATTTTGCACAAGCGCCATGGGGTGGATACAAA
CAATCAGGTATCGGTAGAGAATTAGGCAAAGAAGGCTTAGAAGAGTACCTTGTTTCAAAA
CACATTTTAACAAATACAAATCCACAATTAGTGAATTGGTTTAGCAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2510 [new locus tag: NWMN_RS14400 ]
- symbol: NWMN_2510
- description: glycine betaine aldehyde dehydrogenase
- length: 496
- theoretical pI: 4.70024
- theoretical MW: 54622.4
- GRAVY: -0.306048
⊟Function[edit | edit source]
- reaction: EC 1.2.1.8 ExPASyBetaine-aldehyde dehydrogenase Betaine aldehyde + NAD+ + H2O = betaine + NADH?
- TIGRFAM: Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 775.3)and 14 more5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 483.1)Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 431.5)Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 406.5)1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 401.7)Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 385.2)Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 313.2)putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 299.2)Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 284.2)Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 236.8)Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 212.6)Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 193.8)phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 156.2)acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 51.1)phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 41.3)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 583.2)and 7 moreT3SS-Chaperone (CL0419) YscO-like; YscO-like protein (PF16789; HMM-score: 17.1)ALDH-like (CL0099) DUF1487; Protein of unknown function (DUF1487) (PF07368; HMM-score: 15.7)no clan defined SART-1; SART-1 family (PF03343; HMM-score: 14)Hybrid (CL0105) GARS_C; Phosphoribosylglycinamide synthetase, C domain (PF02843; HMM-score: 12.4)no clan defined DUF1465; Protein of unknown function (DUF1465) (PF07323; HMM-score: 12.2)RPEL; RPEL repeat (PF02755; HMM-score: 10.5)FAM25; FAM25 family (PF15825; HMM-score: 6.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003427
- TAT(Tat/SPI): 0.000197
- LIPO(Sec/SPII): 0.000567
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGTKEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTLEESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPLLQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGDVMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVISTEHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVFGPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPYFAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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