PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2546 [new locus tag: SAUSA300_RS14135 ]
• pan locus tag^?: SAUPAN006314000
• symbol: betB
• pan gene symbol^?: betB
• synonym:
• product: glycine betaine aldehyde dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2546 [new locus tag: SAUSA300_RS14135 ]
• symbol: betB
• product: glycine betaine aldehyde dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 2751267..2752757
• length: 1491
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913279 NCBI
• RefSeq: YP_495180 NCBI
• BioCyc: see SAUSA300_RS14135
• MicrobesOnline: 1294061 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  541
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  841
  901
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  1021
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  1201
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  1441
  
  ATGGAACTTTTAAAACATTTATCTCAGCGTCAATATATTGATGGTGAGTGGGTTGAAAGC
  GCGAATAAAAATACAAGAGATATTATCAATCCTTACAATCAAGAAGTGATATTTACGGTT
  TCTGAAGGGACAAAAGAGGATGCAGAACGTGCAATCTTAGCTGCAAGACGTGCGTTTGAG
  TCTGGTGAATGGTCGCAAGAAACTGCTGAAACAAGAGGTAAAAAAGTACGTGCCATCGCG
  GACAAGATTAAAGAACATCGCGAAGCGTTAGCACGATTAGAAACATTAGATACTGGAAAA
  ACGTTAGAAGAATCATATGCAGATATGGATGATATTCATAATGTGTTTATGTATTTTGCT
  GGATTAGCAGATAAAGACGGTGGCGAAATGATTGATTCACCAATTCCAGATACAGAAAGC
  AAAATTGTTAAAGAACCAGTAGGTGTAGTTACACAAATTACACCTTGGAATTATCCGTTA
  TTACAAGCATCATGGAAAATTGCGCCAGCGCTTGCTACGGGTTGTTCACTAGTTATGAAA
  CCAAGTGAAATTACACCATTAACAACAATACGTGTTTTTGAATTAATGGAAGAAGTTGGT
  TTCCCTAAAGGAACAATTAATCTTATTCTAGGTGCAGGTTCTGAAGTTGGTGACGTAATG
  TCAGGTCATAAAGAGGTTGACCTTGTATCATTTACAGGTGGCATTGAGACTGGTAAGCAT
  ATTATGAAAAATGCTGCTAATAATGTTACGAATATTGCCTTGGAACTTGGCGGTAAAAAT
  CCAAACATTATCTTTGATGATGCTGATTTTGAATTGGCAGTAGACCAAGCGTTAAATGGT
  GGATATTTCCATGCAGGTCAAGTTTGTTCAGCAGGATCAAGAATATTAGTACAAAACAGT
  ATTAAAGACAAATTTGAGCAAGCACTTATTGATCGCGTGAAAAAAATCAAATTAGGTAAT
  GGTTTTGATGCTGATACTGAAATGGGACCAGTGATTTCAACAGAACATCGTAATAAGATC
  GAATCTTATATGGATGTAGCTAAAGCAGAAGGCGCAACAATTGCTGTTGGTGGTAAACGT
  CCAGATAGAGATGATTTAAAAGATGGTCTATTCTTCGAGCCAACAGTCATTACAAATTGT
  GATACGTCAATGCGTATTGTACAAGAAGAGGTTTTCGGACCTGTCGTTACTGTAGAAGGC
  TTTGAAACTGAACAAGAAGCGATTCAATTAGCGAATGATTCTATATATGGTTTAGCAGGT
  GCTGTATTTTCTAAAGATATTGGAAAAGCACAACGCGTTGCTAACAAGTTGAAACTTGGA
  ACGGTGTGGATTAATGATTTCCATCCATATTTTGCACAAGCGCCATGGGGTGGATACAAA
  CAATCAGGTATCGGTAGAGAATTAGGCAAAGAAGGCTTAGAAGAGTACCTTGTTTCAAAA
  CACATTTTAACAAATACAAATCCACAATTAGTGAATTGGTTTAGCAAATAA
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  1491
  

⊟Protein

• 4MPB
  PDB
• 4MPY
  PDB
• 4NEA
  PDB
• 4NU9
  PDB
• 4Q92
  PDB
• 4QJE
  PDB
• 4QN2
  PDB
• 4QTO
  PDB

Protein Data Bank: 4MPB

Protein Data Bank: 4MPY

Protein Data Bank: 4NEA

Protein Data Bank: 4NU9

Protein Data Bank: 4Q92

Protein Data Bank: 4QJE

Protein Data Bank: 4QN2

Protein Data Bank: 4QTO

⊟General

• locus tag: SAUSA300_2546 [new locus tag: SAUSA300_RS14135 ]
• symbol: BetB
• description: glycine betaine aldehyde dehydrogenase
• length: 496
• theoretical pI: 4.70024
• theoretical MW: 54622.4
• GRAVY: -0.306048

⊟Function

• reaction:
  EC 1.2.1.8?  ExPASy
  Betaine-aldehyde dehydrogenase Betaine aldehyde + NAD⁺ + H₂O = betaine + NADH
• TIGRFAM:
  Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 775.3)
  and 14 more
  5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 483.1)
  Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 431.5)
  Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 406.5)
  1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 401.7)
  Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 385.2)
  Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 313.2)
  putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 299.2)
  Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 284.2)
  Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 236.8)
  Metabolism Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 212.6)
  Metabolism Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 193.8)
  phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 156.2)
  acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 51.1)
  phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 41.3)
• TheSEED  :
  • Betaine aldehyde dehydrogenase (EC 1.2.1.8)
  Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  Betaine aldehyde dehydrogenase (EC 1.2.1.8)
• PFAM:
  ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 583.2)
  and 7 more
  T3SS-Chaperone (CL0419) YscO-like; YscO-like protein (PF16789; HMM-score: 17.1)
  ALDH-like (CL0099) DUF1487; Protein of unknown function (DUF1487) (PF07368; HMM-score: 15.7)
  no clan defined SART-1; SART-1 family (PF03343; HMM-score: 14)
  Hybrid (CL0105) GARS_C; Phosphoribosylglycinamide synthetase, C domain (PF02843; HMM-score: 12.4)
  no clan defined DUF1465; Protein of unknown function (DUF1465) (PF07323; HMM-score: 12.2)
  RPEL; RPEL repeat (PF02755; HMM-score: 10.5)
  FAM25; FAM25 family (PF15825; HMM-score: 6.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003427
  • TAT(Tat/SPI): 0.000197
  • LIPO(Sec/SPII): 0.000567
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160618 NCBI
• RefSeq: YP_495180 NCBI
• UniProt: A0A0H2XH11 UniProt

⊟Protein sequence

• MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGTKEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTLEESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPLLQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGDVMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVISTEHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVFGPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPYFAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications