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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2524 [new locus tag: NWMN_RS14505 ]
  • pan locus tag?: SAUPAN006338000
  • symbol: NWMN_2524
  • pan gene symbol?: nsaR
  • synonym: braR
  • product: DNA-binding response regulator

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2524 [new locus tag: NWMN_RS14505 ]
  • symbol: NWMN_2524
  • product: DNA-binding response regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2776080..2776745
  • length: 666
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAATATTAATTGTTGAAGATGATTTTGTTATAGCAGAGAGTTTAGCATCTGAACTT
    AAAAAATGGAATTACGGTGTTATTGTCGTTGAACAATTTGATGATATACTGTCTATCTTT
    AACCAAAATCAACCTCAGCTTGTATTGCTAGATATTAATTTGCCAACGTTAAATGGTTTT
    CATTGGTGTCAAGAAATCCGAAAAACATCTAATGTGCCAATTATATTTATTAGTTCCCGT
    ATTGATAATATGGACCAAATTATGGCAATACAAATGGGGGGAGATGATTTTATCGAAAAG
    CCATTTAACTTGTCATTAACGATTGCCAAAATTCAAGCATTATTGAGACGAACTTATGAC
    TTGTCAGTAGCTAATGATTCATTGACGGTTAAAGGTTGTACATTAATATTAGATGAAGCA
    AAAGTCGTGTATCAAGAACAAAACATACAGCTATCTTTGACTGAATTACAAATATTAAAG
    TTATTATTTCAAAATGAAGATAAATATGTAAGTAGAACTGCTTTAATTGAAAAATGTTGG
    GAATCAGAAAACTTCATAGATGATAACACATTAGCTGTTAACATGACGCGCCTGCGTAAA
    AAATTAAATACTATTGGCGTTAATGATTTTATCATTACAAAGAAAAATGTCGGATATAAA
    GTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    666

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_2524 [new locus tag: NWMN_RS14505 ]
  • symbol: NWMN_2524
  • description: DNA-binding response regulator
  • length: 221
  • theoretical pI: 4.68476
  • theoretical MW: 25392.3
  • GRAVY: 0.0158371

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 159.8)
    Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 159.8)
    and 8 more
    proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 119.7)
    Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 114.1)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 50.3)
    Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 50.3)
    Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 50.3)
    Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 39.2)
    Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 38.7)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 33.5)
  • TheSEED:  
    Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Bacitracin Stress Response  Two-component response regulator YvcP
  • PFAM:
    CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 82.8)
    HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 72.5)
    and 1 more
    HTH_11; HTH domain (PF08279; HMM-score: 11.4)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003491
    • TAT(Tat/SPI): 0.000063
    • LIPO(Sec/SPII): 0.00032
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQPQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPFNLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILKLLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVGYKV

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]