PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2524 [new locus tag: NWMN_RS14505 ]
• pan locus tag^?: SAUPAN006338000
• symbol: NWMN_2524
• pan gene symbol^?: nsaR
• synonym: braR
• product: DNA-binding response regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2524 [new locus tag: NWMN_RS14505 ]
• symbol: NWMN_2524
• product: DNA-binding response regulator
• replicon: chromosome
• strand: -
• coordinates: 2776080..2776745
• length: 666
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331574 NCBI
• RefSeq: YP_001333558 NCBI
• BioCyc:
• MicrobesOnline: 3708126 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAATATTAATTGTTGAAGATGATTTTGTTATAGCAGAGAGTTTAGCATCTGAACTT
  AAAAAATGGAATTACGGTGTTATTGTCGTTGAACAATTTGATGATATACTGTCTATCTTT
  AACCAAAATCAACCTCAGCTTGTATTGCTAGATATTAATTTGCCAACGTTAAATGGTTTT
  CATTGGTGTCAAGAAATCCGAAAAACATCTAATGTGCCAATTATATTTATTAGTTCCCGT
  ATTGATAATATGGACCAAATTATGGCAATACAAATGGGGGGAGATGATTTTATCGAAAAG
  CCATTTAACTTGTCATTAACGATTGCCAAAATTCAAGCATTATTGAGACGAACTTATGAC
  TTGTCAGTAGCTAATGATTCATTGACGGTTAAAGGTTGTACATTAATATTAGATGAAGCA
  AAAGTCGTGTATCAAGAACAAAACATACAGCTATCTTTGACTGAATTACAAATATTAAAG
  TTATTATTTCAAAATGAAGATAAATATGTAAGTAGAACTGCTTTAATTGAAAAATGTTGG
  GAATCAGAAAACTTCATAGATGATAACACATTAGCTGTTAACATGACGCGCCTGCGTAAA
  AAATTAAATACTATTGGCGTTAATGATTTTATCATTACAAAGAAAAATGTCGGATATAAA
  GTATAG
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⊟Protein

⊟General

• locus tag: NWMN_2524 [new locus tag: NWMN_RS14505 ]
• symbol: NWMN_2524
• description: DNA-binding response regulator
• length: 221
• theoretical pI: 4.68476
• theoretical MW: 25392.3
• GRAVY: 0.0158371

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 159.8)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 159.8)
  and 8 more
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 119.7)
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 114.1)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 50.3)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 50.3)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 50.3)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 39.2)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 38.7)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 33.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 82.8)
  HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 72.5)
  and 1 more
  HTH_11; HTH domain (PF08279; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003491
  • TAT(Tat/SPI): 0.000063
  • LIPO(Sec/SPII): 0.00032
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222736 NCBI
• RefSeq: YP_001333558 NCBI
• UniProt: A0A0H3KAS8 UniProt

⊟Protein sequence

• MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQPQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPFNLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILKLLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVGYKV

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2521 < NWMN_2522 < nsaS < NWMN_2524 < NWMN_2525

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications