From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2549 [new locus tag: NWMN_RS14640 ]
  • pan locus tag?: SAUPAN006384000
  • symbol: NWMN_2549
  • pan gene symbol?: asp3
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2549 [new locus tag: NWMN_RS14640 ]
  • symbol: NWMN_2549
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2812122..2813081
  • length: 960
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGCTGAAAAACAAAACATTTAAAGTTACTTGGAATTATATTTCACAAACTACATTTATG
    TATGGCTCTAAAGTATCATTTTCAAATGGTGAAGTGACTTTTATAAATCCTTTGATGCCC
    TCTGGATTACCGATTCATGAGTGGTTGATGTTAAAGCAATTTTCAAAATATAAAAGTGCG
    CCAAGTTTACCAATTTTGCGTAGAGGACAGCATTATAAATTGCATTTTGATTTTGATGCA
    ACGCCTGCTGGTTCAGTGTATTTTATCATCATTTTTTATAATAAAAATGGCACAAAACTA
    AGTACTGAGATTGTAAAGTCAAATAGTATTACGATTCAATATCCTGATGAAGCCTATGCT
    TATAAAATAAAAATGATGAATGCAGCATCGACGAGTCTTATATTTAGATGTTTAACAATC
    ACAGAGATGACGCATCAATATGATTTAGAGTATAAAAGCATGCGGGTGACGAAGATAGGC
    GATGACCAATATGGAAATGATATGATCAATGTCATTATTGCAGAACCATCTGACACCTAT
    CCAACTATTTCGAATGATTTTTTGAAACTGTTTGGTCATGTTTGGCTCGTTGAACGTTGG
    ATGGACGACAATATCGAGGGAAATATTAAGCAACTAAAGAATGATTTGCAAAGTCAAGAT
    ACCCTAAAGGCTATTAATTTGATTAGTTATGGGTCAAAGTCAAATGTTTTTGTAACTTAT
    GTCGCTCAGCATTTGGGTTGCAAAGTGTATCGTACAAGCCATGAAGATGATGATTTAAAA
    GGATGGCTTACAGAACATGTCCCAGGAAATCATGAACTAAAAGATACAAATGTCGAGGTT
    TATTTTAAAGAAGAGCAAGACAAACATCTAAATTATATGTCACGTTTAATGAATCCAGTA
    CGATTTTTAGATTACTTAGATGTATCGAAACTGAATGGTGGGGAAGTAAATGAAACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_2549 [new locus tag: NWMN_RS14640 ]
  • symbol: NWMN_2549
  • description: hypothetical protein
  • length: 319
  • theoretical pI: 6.86271
  • theoretical MW: 36954.9
  • GRAVY: -0.405016

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis accessory Sec system protein Asp3 (TIGR03711; HMM-score: 183)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system protein Asp3 (TIGR03711; HMM-score: 183)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined Sec-ASP3; Accessory Sec secretory system ASP3 (PF15432; HMM-score: 143.8)
    and 1 more
    DUF4884; Domain of unknown function (DUF4884) (PF16225; HMM-score: 13.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022591
    • TAT(Tat/SPI): 0.00063
    • LIPO(Sec/SPII): 0.013038
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLPIHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLSTEIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIGDDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQSQDTLKAINLISYGSKSNVFVTYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDTNVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]