PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2551 [new locus tag: NWMN_RS14650 ]
• pan locus tag^?: SAUPAN006387000
• symbol: NWMN_2551
• pan gene symbol^?: asp1
• synonym:
• product: accessory secretory protein Asp1

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2551 [new locus tag: NWMN_RS14650 ]
• symbol: NWMN_2551
• product: accessory secretory protein Asp1
• replicon: chromosome
• strand: -
• coordinates: 2814617..2816170
• length: 1554
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331591 NCBI
• RefSeq: YP_001333585 NCBI
• BioCyc:
• MicrobesOnline: 3708153 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAATACTTTATTCCAGCTTGGTACGATGACCAACGATGGTGGCAAGACACGACTGTG
  CCGTATTATCAACTACAAAATAAGACGGAATTTGACGATATGATTAGTTTAATGGGAATG
  CACCTTGAAAATGACTTAGATTATCAACTGATTGTTCTCAATCATGCACCAAATTTAAGA
  ACATTTTTACATCGATATGACTTATATGAGACAAAGTATTCGTCTGTGTTTGATGAAATT
  CAAGGATTCAGTCACCATGCGCCACAAGCGATTAATTATCATCACTTAAAATGGCCGGAT
  GATGTTGAGTTTGTGTACACACCGTATTTATTAAAATGTGTGACGAGTGAACAGACCTAT
  ACAAATATTTATTTTAGTCAAGAAGGGTATTCAATTTGGTTTGAAGAATTTGAAAGAGAT
  CAGTTACAACGGCGTTATATTTTTGATGACAGAGGTTATTTATCAGCGATACGTTATTTT
  GATGATCAGGGAGAGGCTTCTTACCAAGAATATTTAACGATTAATGGAGATTGTGTACTT
  CATGAAGATTTGAAAAATGGCAGAGTCACTGTTTCAAAAAGATATCAACATCACTATCAA
  CAAACAGAATATAACAATATGGCTCAACTAATTGAAGAAAAATTTCAAGCAATGATTGCA
  CAACAAATACATGAAGATGATCATGTGATTGTGGCTTCAGATGCTAGGCACAATCGACAA
  ATAGCCAATCATATTCCAGCGAAATCATTAAGTTATTCATTTTTTAAAAATAGAAATGAA
  ACTGTGTCAGATGAGGAATATCAATCTATCGTAAAGAATGCCCATTTAATTGTTGATAGT
  GTGCAACTAGAACGTGATTTAATTAGTCATCAAGAGAAGTATCAGCGGGAGAATACAATG
  ATTCGAATCACACCATTTGAAACGAGACAATCACCTAATATAAGTAGTCAATTGATGGAA
  ACATTTATAGGTGTATGGATAGATGGTATGAGTGACGCTGATTTGCAACAAATGATGCAA
  CGACTTGTGGATTATATCGCACAGGAAGATTATTACCGTTTAATTTTATTATCGCGCCAT
  CAAAATGACATACCGATGTGGCTTCGTGAATGTATTACGTCGGTAAATGAGGAATACCAA
  GCTAAACAGAATGCGGATGTTAATGTTTCAGCATTAATGACACCTGAAGATCAAGATGAC
  ATCATTGCTGTTAAGACGATACATGCTGAACATGATGTTGTAGAAGCATTGCGGACGTTG
  CGACTTGTGATAGATATGTCAAAAGAACCTGACTTGTATTTACAAATTAGTGCAATTAGC
  GCTGGGATTCCACAAATTAATGGTCAACAAACAGATTACGTCTCTGATTATGACAATGGC
  CGTATTATAAATACAGTTGATGAATTAGATGATGCGTTAAATTATTATTTATTTTATTTG
  AAAAATTGGAATTATGCGTACGCCTATTCTTTAAAATTAATAGATGCATATGCTTCTAAG
  AATATTATTAATCAGCTCGATGAGTTAATAGAAGGTGAAAATGATGCCACGTAA
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⊟Protein

⊟General

• locus tag: NWMN_2551 [new locus tag: NWMN_RS14650 ]
• symbol: NWMN_2551
• description: accessory secretory protein Asp1
• length: 517
• theoretical pI: 4.43636
• theoretical MW: 61258.8
• GRAVY: -0.585493

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Pathogenesis accessory Sec system protein Asp1 (TIGR03713; HMM-score: 733.4)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system protein Asp1 (TIGR03713; HMM-score: 733.4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  GT-B (CL0113) Asp1; Accessory Sec system protein Asp1 (PF16993; HMM-score: 360)
  and 2 more
  no clan defined Vasohibin; Vasohibin (PF14822; HMM-score: 15.5)
  Ntox16; Novel toxin 16 (PF15523; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 0
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003065
  • TAT(Tat/SPI): 0.000073
  • LIPO(Sec/SPII): 0.000422
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222763 NCBI
• RefSeq: YP_001333585 NCBI
• UniProt: A0A0H3KAQ2 UniProt

⊟Protein sequence

• MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLENDLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDVEFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYFDDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAMIAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHLIVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDADLQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSALMTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQTDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDELIEGENDAT

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2546 < NWMN_2547 < NWMN_2548 < NWMN_2549 < NWMN_2550 < NWMN_2551 < secY

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications