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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2674 [new locus tag: SACOL_RS14005 ]
  • pan locus tag?: SAUPAN006387000
  • symbol: SACOL2674
  • pan gene symbol?: asp1
  • synonym:
  • product: accessory secretory protein Asp1

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2674 [new locus tag: SACOL_RS14005 ]
  • symbol: SACOL2674
  • product: accessory secretory protein Asp1
  • replicon: chromosome
  • strand: -
  • coordinates: 2745200..2746753
  • length: 1554
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAATACTTTATTCCAGCTTGGTACGATGACCAACGATGGTGGCAAGACACGACTGTG
    CCGTATTATCAACTACAAAATAAGACGGAATTTGACGATATGATTAGTTTAATGGGAATG
    CACCTTGAAAATGACTTAGATTATCAACTGATTGTTCTCAATCATGCACCAAATTTAAGA
    ACATTTTTACATCGATATGACTTATATGAGACAAAGTATTCGTCTGTGTTTGATGAAATT
    CAAGGATTCAGTCACCATGCGCCACAAGCGATTAATTATCATCACTTAAAATGGCCGGAT
    GATGTTGAGTTTGTGTACACACCGTATTTATTAAAATGTGTGACGAGTGAACAGACCTAT
    ACAAATATTTATTTTAGTCAAGAAGGGTATTCAATTTGGTTTGAAGAATTTGAAAGAGAT
    CAGTTACAACGGCGTTATATTTTTGATGACAGAGGTTATTTATCAGCGATACGTTATTTT
    GATGATCAGGGAGAGGCTTCTTACCAAGAATATTTAACGATTAATGGAGATTGTGTACTT
    CATGAAGATTTGAAAAATGGCAGAGTCACTGTTTCAAAAAGATATCAACATCACTATCAA
    CAAACAGAATATAACAATATGGCTCAACTAATTGAAGAAAAATTTCAAGCAATGATTGCA
    CAACAAATACATGAAGATGATCATGTGATTGTGGCTTCAGATGCTAGGCACAATCGACAA
    ATAGCCAATCATATTCCAGCGAAATCATTAAGTTATTCATTTTTTAAAAATAGAAATGAA
    ACTGTGTCAGATGAGGAATATCAATCTATCGTAAAGAATGCCCATTTAATTGTTGATAGT
    GTGCAACTAGAACGTGATTTAATTAGTCATCAAGAGAAGTATCAGCGGGAGAATACAATG
    ATTCGAATCACACCATTTGAAACGAGACAATCACCTAATATAAGTAGTCAATTGATGGAA
    ACATTTATAGGTGTATGGATAGATGGTATGAGTGACGCTGATTTGCAACAAATGATGCAA
    CGACTTGTGGATTATATCGCACAGGAAGATTATTACCGTTTAATTTTATTATCGCGCCAT
    CAAAATGACATACCGATGTGGCTTCGTGAATGTATTACGTCGGTAAATGAGGAATACCAA
    GCTAAACAGAATGCGGATGTTAATGTTTCAGCATTAATGACACCTGAAGATCAAGATGAC
    ATCATTGCTGTTAAGACGATACATGCTGAACATGATGTTGTAGAAGCATTGCGGACGTTG
    CGACTTGTGATAGATATGTCAAAAGAACCTGACTTGTATTTACAAATTAGTGCAATTAGC
    GCTGGGATTCCACAAATTAATGGTCAACAAACAGATTACGTCTCTGATTATGACAATGGC
    CGTATTATAAATACAGTTGATGAATTAGATGATGCGTTAAATTATTATTTATTTTATTTG
    AAAAATTGGAATTATGCGTACGCCTATTCTTTAAAATTAATAGATGCATATGCTTCTAAG
    AATATTATTAATCAGCTCGATGAGTTAATAGAAGGTGAAAATGATGCCACGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2674 [new locus tag: SACOL_RS14005 ]
  • symbol: SACOL2674
  • description: accessory secretory protein Asp1
  • length: 517
  • theoretical pI: 4.43636
  • theoretical MW: 61258.8
  • GRAVY: -0.585493

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis accessory Sec system protein Asp1 (TIGR03713; HMM-score: 733.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system protein Asp1 (TIGR03713; HMM-score: 733.4)
  • TheSEED  :
    • Accessory secretory protein Asp1
    Membrane Transport Protein translocation across cytoplasmic membrane SecY2-SecA2 Specialized Transport System  Accessory secretory protein Asp1
  • PFAM:
    GT-B (CL0113) Asp1; Accessory Sec system protein Asp1 (PF16993; HMM-score: 360)
    and 2 more
    no clan defined Vasohibin; Vasohibin (PF14822; HMM-score: 15.5)
    Ntox16; Novel toxin 16 (PF15523; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003065
    • TAT(Tat/SPI): 0.000073
    • LIPO(Sec/SPII): 0.000422
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLENDLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDVEFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYFDDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAMIAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHLIVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDADLQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSALMTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQTDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDELIEGENDAT

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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