PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2445 [new locus tag: SA_RS14010 ]
• pan locus tag^?: SAUPAN006387000
• symbol: SA2445
• pan gene symbol^?: asp1
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2445 [new locus tag: SA_RS14010 ]
• symbol: SA2445
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2752348..2753901
• length: 1554
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125374 NCBI
• RefSeq: NP_375771 NCBI
• BioCyc: see SA_RS14010
• MicrobesOnline: 104797 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGAAATACTTTATTCCAGCTTGGTACGATGACCAACGATGGTGGCAAGACACGACTGTG
  CCGTATTATCAACTACAAAATAAGACGGAATTTGACGATATGATTAGTTTAATGGGAATG
  CACCTTGAAAATGACTTAGATTATCAACTGATTGTTCTCAATCATGCACCAAATTTAAGA
  ACATTTTTACATCGATATGACTTATATGAAACAAAGTATTCGTCTGTGTTTGATGAAATT
  CAAGGATTCAGTCACCATGCGCCACAAGCGATTAATTATCATCACTTAAAATGGCCGGAT
  GATGTTGAGTTTGTGTACACGCCGTATTTATTAAAATGTGTGACGAGTGAACAGACCTAT
  ACAAATATTTATTTTAGTCAAGAAGGGTATTCAATTTGGTTTGAAGAATTTGAAAGAGAT
  CAGTTACAACGGCGTTATATTTTTGATGACAGAGGTTATTTATCAGCGATACGTTATTTT
  GATGATCAGGGAGAGGCTTCTTACCAACAATATTTAACGATTAATGGAGATTGTGTACTT
  CATGAAGATTTGAAAAATGGCAGAGTCACTGTATCAAAAAGATATCAACATCACTATCAA
  CAAACAGAATATAACAATATGGCTCAACTAATTGAAGAAAAATTTCAAGCAATGATTGCA
  CAACAAATACATGAAGATGATCATGTGATTGTGGCTTCAGATGCTAGGCACAATCGACAA
  ATAGCCAATCATATTCCAGCGAAATTGTTAAGTTATTCATTTTTTAAAAATAGAAATGAA
  ACTGTGTCAGATGAGGAATATCAATCTATCGTAAAGAATGCCCATTTAATTGTTGATAGT
  ATGCAACTAGAACGTGATTTAATTAGTCATCAAGAGAAGTATCAGCGGGAGAATACAATG
  ATTCGAATCACACCATTTGAAACGAGACAATCACCTAATATAAGTAGTCAATTGATGGAA
  ACATTTATAGGTGTATGGATAGATGGTATGAGTGACGCTGATTTGCAACAAATGATGCAA
  CGACTTGTGGATTATATCGCACAGGAAGATTATTACCGTTTAATTTTATTATCGCGCCAT
  CAAAATGACATACCGATGTGGCTTCGTGAATGTATTACGTCGGTAAATGAGGAATACCAA
  GCTAAACAGAATGCGGATGTTAATGTTTCAGCATTAATGACACCTGAAGATCAAGATGAC
  ATCATTGCTGTTAAGACGATACATGCTGAACATGATGTTGTAGAAGCATTGCGGACGTTG
  CGACTTGTGATAGATATGTCAAAAGAACCTGATTTGTATTTACAAATTAGTGCAATTAGC
  GCTGGGATTCCACAAATTAATGGTCAACAAACAGATTACGTCTCTGATTATGACAATGGC
  CGTATTATAAATACAGTTGATGAATTAGATGATGCGTTAAATTATTATTTATTTTATTTG
  AAAAATTGGAATTATGCGTACGCCTATTCTTTAAAATTAATAGATGCATATGCTTCTAAG
  AATATTATTAATCAGCTCGATGAGTTAATAGAAGGTGAAAATGATGCCACGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1554
  

⊟Protein

⊟General

• locus tag: SA2445 [new locus tag: SA_RS14010 ]
• symbol: SA2445
• description: hypothetical protein
• length: 517
• theoretical pI: 4.45506
• theoretical MW: 61315.9
• GRAVY: -0.581044

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Pathogenesis accessory Sec system protein Asp1 (TIGR03713; HMM-score: 730.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system protein Asp1 (TIGR03713; HMM-score: 730.2)
• TheSEED  :
  • Accessory secretory protein Asp1
  Membrane Transport Protein translocation across cytoplasmic membrane SecY2-SecA2 Specialized Transport System  Accessory secretory protein Asp1
• PFAM:
  GT-B (CL0113) Asp1; Accessory Sec system protein Asp1 (PF16993; HMM-score: 359.8)
  and 2 more
  no clan defined Vasohibin; Vasohibin (PF14822; HMM-score: 14.6)
  Ntox16; Novel toxin 16 (PF15523; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 0
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003065
  • TAT(Tat/SPI): 0.000073
  • LIPO(Sec/SPII): 0.000422
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928238 NCBI
• RefSeq: NP_375771 NCBI
• UniProt: A0A0H3JS68 UniProt

⊟Protein sequence

• MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLENDLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDVEFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYFDDQGEASYQQYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAMIAQQIHEDDHVIVASDARHNRQIANHIPAKLLSYSFFKNRNETVSDEEYQSIVKNAHLIVDSMQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDADLQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSALMTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQTDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDELIEGENDAT

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2440 < SA2441 < SA2442 < SA2443 < SA2444 < SA2445 < secY

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications