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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2443 [new locus tag: SA_RS14000 ]
  • pan locus tag?: SAUPAN006384000
  • symbol: SA2443
  • pan gene symbol?: asp3
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2443 [new locus tag: SA_RS14000 ]
  • symbol: SA2443
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2749853..2750893
  • length: 1041
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATGATTCACCTACCATTGCAAGTTGGTTTGTTAATTTTTACAACATTATTTTGGAAG
    ACAAGTTTGGGAGAGTTCAACATGCTGAAAAACAAAACATTTAAAGTTACTTGGAATTAT
    ATTTCACAAACTACATTTATGTATGGCTCTAAAGTATCATTTTCAAATGGTGAAGTGACT
    TTTATAAATCCTTTGATGCCCTCTGGATTACCGATTCATGAGTGGTTGATGTTAAAGCAA
    TTTTCAAAATATAAAAGTGCGCCAAGTTTACCAATTTTGCGTAGAGGACAGCATTATAAA
    TTGCATTTTGATTTTGATGCAACGCCTGCTGGTTCAGTGTATTTTATCATCATTTTTTAT
    AATAAAAATGGCACAAAACTAAGTACTGAGATTGTAAAGTCAAATAGTATTACGATTCAA
    TATCCTGATGAAGCCTATGCTTATAAAATAAAAATGATGAATGCAGCATCGACGAGTCTA
    ATATTTAGATGTTTAACAATTACAGAGATGACGCATCAAGATGATTTAGAGTATAAAAGC
    ATGCGGGTGACGAAGATAGACGATAACCAATATGGAAATGATAGGATCAATGTCATTATT
    GCAGAACCATCTGACACCTATCCAACTATTTCGAATGATTTTTTGAAACTGTTTGGTCAT
    GTTTGGCTCGTTGAACGTTGGATGGACGACGATATCAAGGAAAATATTAAGCAACTAAAG
    GATGATTTGCAAAGTCAAGATACCCTAACGGCTATTAATTTGATTAGTTATGGGTCAAAG
    TCAAATGTTTTTGCAACTTATGTCGCTCAGCATTTAGATTGCAAAGTATATCGTACAAGC
    CATGAAGATGATGATTTAAAAGAATGGCTTACAGAACATGTCCCAGGAAATAATGAACTA
    AAAGATACAAATGTCGAGTTTTATTTTAAAGAAGAGCAAGACAACCATCTAAATTATATG
    TCACGTTTAATGAATCCAGTACGATTTTTAGATTACTTAGATGTATCGAAACTGAATGGT
    GGGGAAGTAAATGAAACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1041

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2443 [new locus tag: SA_RS14000 ]
  • symbol: SA2443
  • description: hypothetical protein
  • length: 346
  • theoretical pI: 6.00309
  • theoretical MW: 40280.6
  • GRAVY: -0.366185

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis accessory Sec system protein Asp3 (TIGR03711; HMM-score: 182.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system protein Asp3 (TIGR03711; HMM-score: 182.8)
  • TheSEED  :
    • Accessory secretory protein Asp3
    Membrane Transport Protein translocation across cytoplasmic membrane SecY2-SecA2 Specialized Transport System  Accessory secretory protein Asp3
  • PFAM:
    no clan defined Sec-ASP3; Accessory Sec secretory system ASP3 (PF15432; HMM-score: 143.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.5
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 24 aa
    • SP(Sec/SPI): 0.49665
    • TAT(Tat/SPI): 0.002242
    • LIPO(Sec/SPII): 0.012857
    • Cleavage Site: CS pos: 24-25. SLG-EF. Pr: 0.4268
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MMIHLPLQVGLLIFTTLFWKTSLGEFNMLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLPIHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLSTEIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDDLEYKSMRVTKIDDNQYGNDRINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDDIKENIKQLKDDLQSQDTLTAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWLTEHVPGNNELKDTNVEFYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]