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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2669 [new locus tag: SACOL_RS13980 ]
  • pan locus tag?: SAUPAN006380000
  • symbol: SACOL2669
  • pan gene symbol?: gtfB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2669 [new locus tag: SACOL_RS13980 ]
  • symbol: SACOL2669
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2737448..2738806
  • length: 1359
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATTAAATTATTTGATTACTTTAACGATCATTCACGAAAGTTATATGAGTCTTTTAAA
    GCCAGTAAGTTGGAAGAAGATTTAACAATTGTACTCAATGACAATGGCTTTTTACCGGAC
    GATGTTATATCACCGTATCAATTTTTTGCAGATAATCATAATACAGAAAATATGAAACCA
    CGATTTTTTAATCAGGTTACTGTACCAGCTTTTTGGGAAATTAAAGGTAACAACAATTCG
    GCTACAATTAATGATATGGGACGTCTACGAGGTAAAATATTTTATCAAAGTGGAGAGCGC
    CCTAGAATTGTTAGTCGTGTCGAATGGTTTGATGACCAACAACGTGTCCGATTTGTCGAT
    TATTATTCTAAGAATGGTATTAAATTTGCACAAACTGTTTACGATTTAAATCGTAAAGCG
    ATCTTGAAGAAATATATGACAGCAGAAGGTAAAGAAGTGATTTATGAAAATTTTGTAACA
    TCTGATGTCATATTGGATTGGCAAGGGAAGTCTTACTTTTTCCCTTCAAAGCTCGCATTC
    GCATTATTTTTTATCAAGCAACTTGAAATTACGGAACATCATTTCGTTATTAACTCACTA
    GCCCTACCATTTTCAGTGTTATATAATTTGCCATCAAAAGGTAGTGATGTTTTGGTATGG
    CAAGAACACTGTAATGGTAATGTCCCGGGAAATATGCAATTGATGTGTAAAGGTGATATG
    AAACGACATTGTAATATTATTATTCCAGATAAAAACGAATATGAAACGATGCTCAATATA
    GCAGACGCAAAGGTTCAATCTCGTATTTTACAAGGCGGATATCTATATAACTATCGTAGT
    CGTAATAGATATACTAAAGAAATTGTAATATTAACTAACTCGGATCAACTTCGTAACATC
    AAGGTATTGGTTGAAACTTTACCAGATTTTAAGTTTCACATCGCTGCTATTACAGAGATG
    TCTGATAAGCTAATGCAACTTGATCAATATGCTAATGTGCATTTATACCCATCTATTAAT
    ATTGACAGGGTAAATGAACTATACCAGTTATGTGATATTTATCTTGATATCAATGAGGGT
    AATGAAATTTTAAATGCGGTGGAACAAGCGTTTGACTATGAGTTGTTGATTTTAGGTTAT
    CGTCAAACCGCACACCATGCTAAAGTAACATTATCAGAGCACTTATTTGAGCATAATGAT
    GAGATAACAATGGAAAGTAAAGACCAATTAATACAAATGTTAGAAAGTTTGAAAGATCAA
    CAACAGTTTCGAGATGCTTTATTAGCACAAAAAGCACATGCACACGAAATCTCGCGTGAA
    CAATTCGAGCAAGTTTTTAAACAGGCGCTCGAAAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2669 [new locus tag: SACOL_RS13980 ]
  • symbol: SACOL2669
  • description: hypothetical protein
  • length: 452
  • theoretical pI: 5.89667
  • theoretical MW: 53027.9
  • GRAVY: -0.417035

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosyltransferase GtfB (TIGR02919; EC 2.4.1.-; HMM-score: 664.4)
  • TheSEED  :
    • GftB: Glycosyl transferase, family 8
    Membrane Transport Protein translocation across cytoplasmic membrane SecY2-SecA2 Specialized Transport System  GftB: Glycosyl transferase, family 8
  • PFAM:

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001797
    • TAT(Tat/SPI): 0.000134
    • LIPO(Sec/SPII): 0.000283
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVISPYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPRIVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVTSDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVLVWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLYNYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVHLYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLSEHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQALES

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell: 50 [2]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [3] [4]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  3. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  4. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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