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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2673 [new locus tag: SACOL_RS14000 ]
  • pan locus tag?: SAUPAN006385000
  • symbol: SACOL2673
  • pan gene symbol?: asp2
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2673 [new locus tag: SACOL_RS14000 ]
  • symbol: SACOL2673
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2743642..2745210
  • length: 1569
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCACGTAAATTTAGAGTTTTGCAAATTGGAGGAGACGATTTAGAACCTATTTTTCAA
    CACAAAAAAGGTGTGAGTTGGGATTACTTCGATATTGGATTGTTTGAATTTGATAGTGGT
    TATGTAGAGGCTATTGAAGCGATTGTTGAAGCAGAAGGGCGCTTTGATTTTATCTATATT
    CAAGCACCATACTCGGAGACATTAACGAATTTATTACAAATGATAAGCGAACCATACAAT
    ACGTATGTTGATGAATCATTTTGGTCAGTTGAATATGAACAAGACGAAAATGTCCAAAAA
    TACGTTGTTCAACCATTACATTACCGGAATATTGAAGAACGTAATAATAAATTAGAGGCA
    GTTAGCTTCTCAGGACAGTATGGAGATAAGGTTTCTCCTAAGTTAGCACTTGTGCATCCG
    AATTTTAAAGGAGATGTCGTTTACCAAGGTAATTCAGAGCTCACATTGAGTGGAGAATTT
    AGGAAAGAATTTAAACCTATCGCATCTTGGCAAAATAATCTCGTTTACGATAAAGATAAA
    GTCATTCAAATATGGCCAGAATTTGATATTGATGGTGCGGTCGAGTTGCAATATACATTT
    AGATTGATTCAGACTGGCGCTGATGGTGCATTAATTGAACAAATCGTATTGACTGATGAT
    ATGTTAGACAGTCCTTTAGAGATACCTGCGAAACCATTTGATGCTTATATAAGTGTAACT
    GTTAAGGCGCGTGGGAACGGGACGGTACATTTAGGACCTATACACAAACGATGGTCTAGA
    TTAGACATGGGTCAATTTTTACTTGGTGGTAGTCGTTTCGTAGATAGTCAACGACAGGAA
    TTTATTTATTATTTCCACCCTGGTGATATGAAACCGCCACTAAACGTATATTTTAGTGGT
    TATCGAACAGCGGAAGGTTTCGAAGGATATTATATGATGAAACGTATGAATGCACCGTTT
    TTACTTATCGGCGATCCTCGTGTTGAAGGTGGTAGTTTTTATATCGGTTCATCTGAATAT
    GAACAAGGTATTATCAATGTTATTGACGAGACATTAGAAAAGCTGAATTTTAAATCACAT
    GAATTAATATTATCTGGTTTATCTATGGGTTCATTTGGTGCCTTGTATTATGGTGCTCAG
    TTAAATCCTCAGGCAATTATTGTTGGTAAACCACTTGTTAATATTGGAACGATTGCTGAA
    CATATGCGATTACTTAGACCAGAAGAGTTCGGTACAGCATTAGACGTCCTGGTGTCTAAT
    GAAGGTGATACATCTCAAGCGTCGATACAGGCATTAAATCAAAAATTTTGGCAAACATTT
    CAAAAGAAATCATTGTCACAAACTGTTTTTGCCATAGCATATATGCAACATGATGATTAT
    GATCCACATGCATTTCAAGAGCTATTACCTGTATTGACAGCACACCAAGCAAGAGTGATG
    AATCGCAGTATTCCTGGGCGACATAATGATGATTCACCTACCATTGCAAGTTGGTTTGTT
    AATTTTTACAACATTATTTTGGAAGACAAGTTTGGGAGAGTTCAACATGCTGAAAAACAA
    AACATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1569

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2673 [new locus tag: SACOL_RS14000 ]
  • symbol: SACOL2673
  • description: hypothetical protein
  • length: 522
  • theoretical pI: 4.73857
  • theoretical MW: 59668.9
  • GRAVY: -0.312644

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis accessory Sec system protein Asp2 (TIGR03712; HMM-score: 804.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system protein Asp2 (TIGR03712; HMM-score: 804.9)
    and 1 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 12.5)
  • TheSEED  :
    • Accessory secretory protein Asp2
    Membrane Transport Protein translocation across cytoplasmic membrane SecY2-SecA2 Specialized Transport System  Accessory secretory protein Asp2
  • PFAM:
    AB_hydrolase (CL0028) Asp2; Accessory Sec system GspB-transporter (PF16929; HMM-score: 717.8)
    and 4 more
    no clan defined DUF3741; Protein of unknown function (DUF3741) (PF12552; HMM-score: 13.1)
    DUF3473; Domain of unknown function (DUF3473) (PF11959; HMM-score: 12.9)
    Viral_Gag (CL0148) Gag_p24; gag gene protein p24 (core nucleocapsid protein) (PF00607; HMM-score: 11.5)
    AB_hydrolase (CL0028) PAE; Pectinacetylesterase (PF03283; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002767
    • TAT(Tat/SPI): 0.000127
    • LIPO(Sec/SPII): 0.000277
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEAIEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYVVQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEFRKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLTDDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDSQRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFYIGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPLVNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVFAIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILEDKFGRVQHAEKQNI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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