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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02987
  • pan locus tag?: SAUPAN006385000
  • symbol: SAOUHSC_02987
  • pan gene symbol?: asp2
  • synonym:
  • product: accessory Sec system protein Asp2

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02987
  • symbol: SAOUHSC_02987
  • product: accessory Sec system protein Asp2
  • replicon: chromosome
  • strand: -
  • coordinates: 2755466..2757034
  • length: 1569
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCACGTAAATTTAGAGTTTTGCAAATTGGAGGAGACGATTTAGAACCTATTTTTCAA
    CACAAAAAAGGTGTGAGTTGGGATTACTTCGATATTGGATTGTTTGAATTTGATAGTGGT
    TATGTAGAGGCTATTGAAGCGATTGTTGAAGCAGAAGGGCGCTTTGATTTTATCTATATT
    CAAGCACCATACTCGGAGACATTAACGAATTTATTACAAATGATAAGCGAACCATACAAT
    ACGTATGTTGATGAATCATTTTGGTCAGTTGAATATGAACAAGACGAAAATGTCCAAAAA
    TACGTTGTTCAACCATTACATTACCGGAATATTGAAGAACGTAATAATAAATTAGAGGCA
    GTTAGCTTCTCAGGACAGTATGGAGATAAGGTTTCTCCTAAGTTAGCACTTGTGCATCCG
    AATTTTAAAGGAGATGTCGTTTACCAAGGTAATTCAGAGCTCACATTGAGTGGAGAATTT
    GGGAAAGAATTTAAACCTATCGCATCTTGGCAAAATAATCTCGTTTACGATAAAGATAAA
    GTCATTCAAATATGGCCAGAATTTGATATTGATGGTGCGGTCGAGTTGCAATATACATTT
    AGATTGATTCAGACTGGCGCTGATGGTGCATTAATTGAACAAATCGTATTGACTGATGAT
    ATGTTAGACAGTCCTTTAGAGATACCTGCGAAACCATTTGATGCTTATATAAGTGTAACT
    GTTAAGGCGCGTGGGAACGGGACGGTACATTTAGGACCTATACACAAACGATGGTCTAGA
    TTAGACATGGGTCAATTTTTACTTGGTGGTAGTCGTTTCGTAGATAGTCAACGACAGGAA
    TTTATTTATTATTTCCACCCTGGTGATATGAAACCGCCACTAAACGTATATTTTAGTGGT
    TATCGAACAGCGGAAGGTTTCGAAGGATATTATATGATGAAACGTATGAATGCACCGTTT
    TTACTTATCGGCGATCCTCGTGTTGAAGGTGGTAGTTTTTATATCGGTTCATCTGAATAT
    GAACAAGGTATTATCAATGTTATTGACGAGACATTAGAAAAGCTGAATTTTAAATCACAT
    GAATTAATATTATCTGGTTTATCTATGGGTTCATTTGGTGCCTTGTATTATGGTGCTCAG
    TTAAATCCTCAGGCAATTATTGTTGGTAAACCACTTGTTAATATTGGAACGATTGCTGAA
    CATATGCGATTACTTAGACCAGAAGAGTTCGGTACAGCATTAGACGTCCTGGTGTCTAAT
    GAAGGTGATACATCTCAAGCGTCGATACAGGCATTAAATCAAAAATTTTGGCAAACATTT
    CAAAAGAAATCATTGTCACAAACTGTTTTTGCCATAGCATATATGCAACATGATGATTAT
    GATCCACATGCATTTCAAGAGCTATTACCTGTATTGACAGCACACCAAGCAAGAGTGATG
    AATCGCAGTATTCCTGGGCGACATAATGATGATTCACCTACCATTGCAAGTTGGTTTGTT
    AATTTTTACAACATTATTTTGGAAGACAAGTTTGGGAGAGTTCAACATGCTGAAAAACAA
    AACATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1569

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02987
  • symbol: SAOUHSC_02987
  • description: accessory Sec system protein Asp2
  • length: 522
  • theoretical pI: 4.69473
  • theoretical MW: 59569.8
  • GRAVY: -0.304789

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis accessory Sec system protein Asp2 (TIGR03712; HMM-score: 805.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system protein Asp2 (TIGR03712; HMM-score: 805.8)
    and 1 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 12.5)
  • TheSEED  :
    • Accessory secretory protein Asp2
    Membrane Transport Protein translocation across cytoplasmic membrane SecY2-SecA2 Specialized Transport System  Accessory secretory protein Asp2
  • PFAM:
    AB_hydrolase (CL0028) Asp2; Accessory Sec system GspB-transporter (PF16929; HMM-score: 720.4)
    and 4 more
    no clan defined DUF3741; Protein of unknown function (DUF3741) (PF12552; HMM-score: 13.1)
    DUF3473; Domain of unknown function (DUF3473) (PF11959; HMM-score: 12.9)
    Viral_Gag (CL0148) Gag_p24; gag gene protein p24 (core nucleocapsid protein) (PF00607; HMM-score: 11.5)
    AB_hydrolase (CL0028) PAE; Pectinacetylesterase (PF03283; HMM-score: 11.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002767
    • TAT(Tat/SPI): 0.000127
    • LIPO(Sec/SPII): 0.000277
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEAIEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYVVQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEFGKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLTDDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDSQRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFYIGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPLVNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVFAIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILEDKFGRVQHAEKQNI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Ian R Siboo, Donald O Chaffin, Craig E Rubens, Paul M Sullam
Characterization of the accessory Sec system of Staphylococcus aureus.
J Bacteriol: 2008, 190(18);6188-96
[PubMed:18621893] [WorldCat.org] [DOI] (I p)

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