PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS14000 [old locus tag: SACOL2673 ]
• pan locus tag^?: SAUPAN006385000
• symbol: SACOL_RS14000
• pan gene symbol^?: asp2
• synonym:
• product: accessory Sec system protein Asp2

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS14000 [old locus tag: SACOL2673 ]
• symbol: SACOL_RS14000
• product: accessory Sec system protein Asp2
• replicon: chromosome
• strand: -
• coordinates: 2743642..2745210
• length: 1569
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2743642..2745210) NCBI
• BioCyc: SACOL_RS14000 BioCyc
• MicrobesOnline: see SACOL2673

⊟Phenotype

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⊟DNA sequence

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  ATGCCACGTAAATTTAGAGTTTTGCAAATTGGAGGAGACGATTTAGAACCTATTTTTCAA
  CACAAAAAAGGTGTGAGTTGGGATTACTTCGATATTGGATTGTTTGAATTTGATAGTGGT
  TATGTAGAGGCTATTGAAGCGATTGTTGAAGCAGAAGGGCGCTTTGATTTTATCTATATT
  CAAGCACCATACTCGGAGACATTAACGAATTTATTACAAATGATAAGCGAACCATACAAT
  ACGTATGTTGATGAATCATTTTGGTCAGTTGAATATGAACAAGACGAAAATGTCCAAAAA
  TACGTTGTTCAACCATTACATTACCGGAATATTGAAGAACGTAATAATAAATTAGAGGCA
  GTTAGCTTCTCAGGACAGTATGGAGATAAGGTTTCTCCTAAGTTAGCACTTGTGCATCCG
  AATTTTAAAGGAGATGTCGTTTACCAAGGTAATTCAGAGCTCACATTGAGTGGAGAATTT
  AGGAAAGAATTTAAACCTATCGCATCTTGGCAAAATAATCTCGTTTACGATAAAGATAAA
  GTCATTCAAATATGGCCAGAATTTGATATTGATGGTGCGGTCGAGTTGCAATATACATTT
  AGATTGATTCAGACTGGCGCTGATGGTGCATTAATTGAACAAATCGTATTGACTGATGAT
  ATGTTAGACAGTCCTTTAGAGATACCTGCGAAACCATTTGATGCTTATATAAGTGTAACT
  GTTAAGGCGCGTGGGAACGGGACGGTACATTTAGGACCTATACACAAACGATGGTCTAGA
  TTAGACATGGGTCAATTTTTACTTGGTGGTAGTCGTTTCGTAGATAGTCAACGACAGGAA
  TTTATTTATTATTTCCACCCTGGTGATATGAAACCGCCACTAAACGTATATTTTAGTGGT
  TATCGAACAGCGGAAGGTTTCGAAGGATATTATATGATGAAACGTATGAATGCACCGTTT
  TTACTTATCGGCGATCCTCGTGTTGAAGGTGGTAGTTTTTATATCGGTTCATCTGAATAT
  GAACAAGGTATTATCAATGTTATTGACGAGACATTAGAAAAGCTGAATTTTAAATCACAT
  GAATTAATATTATCTGGTTTATCTATGGGTTCATTTGGTGCCTTGTATTATGGTGCTCAG
  TTAAATCCTCAGGCAATTATTGTTGGTAAACCACTTGTTAATATTGGAACGATTGCTGAA
  CATATGCGATTACTTAGACCAGAAGAGTTCGGTACAGCATTAGACGTCCTGGTGTCTAAT
  GAAGGTGATACATCTCAAGCGTCGATACAGGCATTAAATCAAAAATTTTGGCAAACATTT
  CAAAAGAAATCATTGTCACAAACTGTTTTTGCCATAGCATATATGCAACATGATGATTAT
  GATCCACATGCATTTCAAGAGCTATTACCTGTATTGACAGCACACCAAGCAAGAGTGATG
  AATCGCAGTATTCCTGGGCGACATAATGATGATTCACCTACCATTGCAAGTTGGTTTGTT
  AATTTTTACAACATTATTTTGGAAGACAAGTTTGGGAGAGTTCAACATGCTGAAAAACAA
  AACATTTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS14000 [old locus tag: SACOL2673 ]
• symbol: SACOL_RS14000
• description: accessory Sec system protein Asp2
• length: 522
• theoretical pI: 4.73857
• theoretical MW: 59668.9
• GRAVY: -0.312644

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Pathogenesis accessory Sec system protein Asp2 (TIGR03712; HMM-score: 804.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system protein Asp2 (TIGR03712; HMM-score: 804.9)
  and 1 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 12.5)
• TheSEED: see SACOL2673
• PFAM:
  AB_hydrolase (CL0028) Asp2; Accessory Sec system GspB-transporter (PF16929; HMM-score: 717.8)
  and 4 more
  no clan defined DUF3741; Protein of unknown function (DUF3741) (PF12552; HMM-score: 13.1)
  DUF3473; Domain of unknown function (DUF3473) (PF11959; HMM-score: 12.9)
  Viral_Gag (CL0148) Gag_p24; gag gene protein p24 (core nucleocapsid protein) (PF00607; HMM-score: 11.5)
  AB_hydrolase (CL0028) PAE; Pectinacetylesterase (PF03283; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002767
  • TAT(Tat/SPI): 0.000127
  • LIPO(Sec/SPII): 0.000277
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447060221 NCBI
• RefSeq: WP_001137477 NCBI
• UniProt: see SACOL2673

⊟Protein sequence

• MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEAIEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYVVQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEFRKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLTDDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDSQRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFYIGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPLVNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVFAIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILEDKFGRVQHAEKQNI

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: see SACOL2673
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications