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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02165 [old locus tag: NWMN_0383 ]
  • pan locus tag?: SAUPAN002056000
  • symbol: NWMN_RS02165
  • pan gene symbol?: —
  • synonym:
  • product: MazF/PemK family toxin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS02165 [old locus tag: NWMN_0383 ]
  • symbol: NWMN_RS02165
  • product: MazF/PemK family toxin
  • replicon: chromosome
  • strand: -
  • coordinates: 432025..432729
  • length: 705
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (432025..432729) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0383

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTCTAAAAATATCAATCAAGCAAATAGTAAATTAAATACGTCAAACAAAAAACTAAAA
    CAAGCTTATTCAAAATCCGACAGTAAAAACATGAAAGTTATATACATGCCTCATTGGCTA
    GAGTTTTATTCAATTGCTATACATAGCGATGTTACAAGCAATAAAAAAAGATATTATAAA
    TCATATAGTAGAGGAACAGTGGTATATGTAAAACTAGGGAGTAATATTGGAAGTGAGTTT
    TCTGGTAATCATTTTTGTGTAATATTAGATAACAAAGACAATAAAGGAAAAGAAACAGTT
    ACAATAGTTCCACTTTCTTCCAAAGGTAATAAAAATTATTTGAAGTTAAACGAATCGGTG
    CTAAATCTAACAGCAACAGATTTAAAAAAACAGATTATCGATATCAGTAGTAAAGTACAA
    GCATTAGCCCAAAAATATAGAGAAATTGACATTAAACTTAGCACAGAAGATAAAAAGCTT
    TTGTCTAACTTAAATCAAAAAGCTAATGAACTGTATAGAGTGATAGGTGTTTATTCCAAG
    CACAAAGGGAAAGATACATACGTTAATATATCAGCAATTACAACAATAAGCAAAAGACGT
    ATAAGCAAAATAAATGATTCTGATCCTACTGGAACGATAATAATTTCAGAAGATGACATG
    AATGAAATCGAAAAGCAATTAAAGATAAAGTTCTTTTCTAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS02165 [old locus tag: NWMN_0383 ]
  • symbol: NWMN_RS02165
  • description: MazF/PemK family toxin
  • length: 234
  • theoretical pI: 10.2746
  • theoretical MW: 26618.3
  • GRAVY: -0.623077

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    CcdB_PemK (CL0624) PemK_toxin; PemK-like, MazF-like toxin of type II toxin-antitoxin system (PF02452; HMM-score: 52.4)
    and 4 more
    GCS (CL0286) Glu_cys_ligase; Glutamate-cysteine ligase (PF04262; HMM-score: 15.6)
    no clan defined Muted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 10)
    Spc24; Spc24 subunit of Ndc80 (PF08286; HMM-score: 8.3)
    DASH_Dad4; DASH complex subunit Dad4 (PF08650; HMM-score: 7.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0158
    • TAT(Tat/SPI): 0.000964
    • LIPO(Sec/SPII): 0.002292
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYSIAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTIVPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKLLSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISEDDMNEIEKQLKIKFFSN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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