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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03180 [old locus tag: NWMN_0555 ]
  • pan locus tag?: SAUPAN002373000
  • symbol: NWMN_RS03180
  • pan gene symbol?: mvaK2
  • synonym:
  • product: phosphomevalonate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS03180 [old locus tag: NWMN_0555 ]
  • symbol: NWMN_RS03180
  • product: phosphomevalonate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 638971..640047
  • length: 1077
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (638971..640047) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0555

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTCAGGTCAAAGCACCCGGAAAACTTTATATTGCTGGAGAATATGCTGTAACAGAA
    CCAGGATATAAATCTGTACTTATTGCGTTAGATCGTTTTGTAACTGCTACTATTGAAGAA
    GCAGACCAATATAAAGGTACCATTCATTCAAAAGCATTACATCATAACCCAGTTACATTT
    AGTAGAGATGAAGATAGTATTGTCATTTCAGATCCACATGCAGCAAAACAATTAAATTAT
    GTGGTCACAGCTATTGAAATATTTGAACAATACGCGAAAAGTTGCGATATAGCGATGAAG
    CATTTTCATCTGACTATTGATAGTAATTTAGATGATTCAAATGGTCATAAATATGGATTA
    GGTTCAAGTGCAGCAGTACTTGTGTCAGTTATAAAAGTATTAAATGAATTTTATGATATG
    AAGTTATCTAATTTATACATTTATAAACTAGCAGTGATTGCAAATATGAAGTTACAAAGT
    TTAAGTTCATGCGGAGATATTGCTGTGAGTGTATATAGTGGATGGCTAGCGTATAGTACT
    TTTGATCATGAATGGGTTAAGCATCAAATTGAAGATACTACGGTTGAAGAAGTTTTAATC
    AAAAACTGGCCTGGATTGCACATCGAACCATTACAAGCACCTGAAAATATGGAAGTACTT
    ATCGGTTGGACTGGCTCACCGGCGTCATCACCACACTTTGTTAGCGAAGTGAAACGTTTG
    AAATCAGATCCTTCATTTTACGGTGACTTCTTAGAAGATTCACATCGTTGTGTTGAAAAA
    CTTATTCATGCTTTTAAAACAAATAACATTAAAGGTGTGCAAAAGATGGTGCGTCAGAAT
    CGTACAATTATTCAACGTATGGATAAAGAAGCTACAGTTGATATAGAAACTGAAAAGCTA
    AAATATTTGTGTGATATTGCTGAAAAGTATCACGGCGCATCTAAAACATCAGGCGCTGGT
    GGTGGAGACTGTGGTATTACAATTATCAATAAAGATGTAGATAAAGAAAAAATTTATGAT
    GAATGGACAAAACATGGTATTAAACCATTAAAATTTAATATTTATCATGGGCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1077

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS03180 [old locus tag: NWMN_0555 ]
  • symbol: NWMN_RS03180
  • description: phosphomevalonate kinase
  • length: 358
  • theoretical pI: 6.50932
  • theoretical MW: 40216.6
  • GRAVY: -0.272626

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 584.7)
    and 5 more
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 92.4)
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01219; EC 2.7.4.2; HMM-score: 59.6)
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 22.1)
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 13.7)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 12.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 48.6)
    no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 42.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004989
    • TAT(Tat/SPI): 0.000323
    • LIPO(Sec/SPII): 0.000758
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQYKGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHFHLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQSLSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENMEVLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKMVRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVDKEKIYDEWTKHGIKPLKFNIYHGQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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