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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05280 [old locus tag: NWMN_0943 ]
  • pan locus tag?: SAUPAN003289000
  • symbol: NWMN_RS05280
  • pan gene symbol?: thiX
  • synonym:
  • product: energy-coupling factor transporter transmembrane protein EcfT

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS05280 [old locus tag: NWMN_0943 ]
  • symbol: NWMN_RS05280
  • product: energy-coupling factor transporter transmembrane protein EcfT
  • replicon: chromosome
  • strand: -
  • coordinates: 1047594..1048400
  • length: 807
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (1047594..1048400) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0943

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTATGAGCAATGGAAAGTTCGGCAATCATTTGTTGATGATGTCAACATTATAACGAAA
    CTATTTCTTGGAATTATACTATTTTTCTTTATTATATTTGTGCATAATTTCGATTTTATG
    CTTTATATCACTATCTTAATGTTAGTTTTCTTATTGATGTTTAATGGTACTCAGTTTAAA
    ATTACAGCAATTTTTATAACTATAAGTATTGGCTTTGCATTACTCTCTTCCCTGTTCATG
    ATTTTATACGGGAATGGTAGCCATACAATTTTGAAATTCGGATTTATCCATATTACAACA
    GAAAGTTTAGTTCGTGGTTTACATGTATCATTAAGAACCATTGCTATTTCATTTTTCGGT
    ATCTTAATCGCACTTACATCTCAGGTTGTCATGATATTTTATAGTTTAATGCAACATTTA
    AAGGTTAAATCAAAAGTTGCTTATGCATTCATGGCTGCCATTCGTATGATACCTTTAATG
    ATTAGTTCTTTAATACAGCTTCGCAGATCTTTAAAAATGCGCTATCAGATGATAGACGCG
    GCAAATTATAGAGGGTTAAAGCGATTTAAGCATTTAATTATACCTCTATTAAGCCAAAAT
    ATTAGAAAGGCACATCAATTATCAGTTGCGATGGAGTCGAAAGGATTTAGAGATGGTCCT
    CGTACTTACTATTACAACGTACCTTTCTCATATAAAGATATAATCTTTATTATAGTTTTA
    ATTGCCATTATTACTATGGCATATTATTGTTCAATCACCTTACCCATCACGGGCATAGAT
    GATGTTCGTCTTGGTCGTGTAGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    807

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS05280 [old locus tag: NWMN_0943 ]
  • symbol: NWMN_RS05280
  • description: energy-coupling factor transporter transmembrane protein EcfT
  • length: 268
  • theoretical pI: 10.5187
  • theoretical MW: 30953.2
  • GRAVY: 0.758582

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ECF transporter T component CbiQ (TIGR02454; HMM-score: 53.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined CbiQ; Cobalt transport protein (PF02361; HMM-score: 82.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000557
    • TAT(Tat/SPI): 0.000079
    • LIPO(Sec/SPII): 0.004336
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYITILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTESLVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLMISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRDGPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]