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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS05280 [old locus tag: NWMN_0943 ]
- pan locus tag?: SAUPAN003289000
- symbol: NWMN_RS05280
- pan gene symbol?: thiX
- synonym:
- product: energy-coupling factor transporter transmembrane protein EcfT
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS05280 [old locus tag: NWMN_0943 ]
- symbol: NWMN_RS05280
- product: energy-coupling factor transporter transmembrane protein EcfT
- replicon: chromosome
- strand: -
- coordinates: 1047594..1048400
- length: 807
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGTATGAGCAATGGAAAGTTCGGCAATCATTTGTTGATGATGTCAACATTATAACGAAA
CTATTTCTTGGAATTATACTATTTTTCTTTATTATATTTGTGCATAATTTCGATTTTATG
CTTTATATCACTATCTTAATGTTAGTTTTCTTATTGATGTTTAATGGTACTCAGTTTAAA
ATTACAGCAATTTTTATAACTATAAGTATTGGCTTTGCATTACTCTCTTCCCTGTTCATG
ATTTTATACGGGAATGGTAGCCATACAATTTTGAAATTCGGATTTATCCATATTACAACA
GAAAGTTTAGTTCGTGGTTTACATGTATCATTAAGAACCATTGCTATTTCATTTTTCGGT
ATCTTAATCGCACTTACATCTCAGGTTGTCATGATATTTTATAGTTTAATGCAACATTTA
AAGGTTAAATCAAAAGTTGCTTATGCATTCATGGCTGCCATTCGTATGATACCTTTAATG
ATTAGTTCTTTAATACAGCTTCGCAGATCTTTAAAAATGCGCTATCAGATGATAGACGCG
GCAAATTATAGAGGGTTAAAGCGATTTAAGCATTTAATTATACCTCTATTAAGCCAAAAT
ATTAGAAAGGCACATCAATTATCAGTTGCGATGGAGTCGAAAGGATTTAGAGATGGTCCT
CGTACTTACTATTACAACGTACCTTTCTCATATAAAGATATAATCTTTATTATAGTTTTA
ATTGCCATTATTACTATGGCATATTATTGTTCAATCACCTTACCCATCACGGGCATAGAT
GATGTTCGTCTTGGTCGTGTAGGTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS05280 [old locus tag: NWMN_0943 ]
- symbol: NWMN_RS05280
- description: energy-coupling factor transporter transmembrane protein EcfT
- length: 268
- theoretical pI: 10.5187
- theoretical MW: 30953.2
- GRAVY: 0.758582
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds cobalt ECF transporter T component CbiQ (TIGR02454; HMM-score: 53.8)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined CbiQ; Cobalt transport protein (PF02361; HMM-score: 82.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 5
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.000557
- TAT(Tat/SPI): 0.000079
- LIPO(Sec/SPII): 0.004336
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYITILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTESLVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLMISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRDGPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: THI-box see NWMN_0943
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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