PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS05425 [old locus tag: NWMN_0968 ]
• pan locus tag^?: SAUPAN003326000
• symbol: NWMN_RS05425
• pan gene symbol^?: potD
• synonym:
• product: spermidine/putrescine ABC transporter substrate-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS05425 [old locus tag: NWMN_0968 ]
• symbol: NWMN_RS05425
• product: spermidine/putrescine ABC transporter substrate-binding protein
• replicon: chromosome
• strand: +
• coordinates: 1073841..1074914
• length: 1074
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1073841..1074914) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0968

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGAAACGATTTTTACAACTCATTATAGGTGCATTAGTTGTGGGTATGCTTTGTCTTACT
  TTAAGTCATTGGTTTAAATCTAAAGAACAAGTGCATACAAATCAAAAAATTTACGTATAC
  AATTGGGGCGAATATATTGATCCAGAGTTAATTAAGAAATTTGAAAAAGAAACTGGCATT
  CAAGTCGTTTATGAAACTTTCGATTCAAATGAAGCGATGGAAGCCAAAATTCGCAATGGC
  GGTACACATTATGATGTTGCTTTTCCTAGTGAATATACAGTTCAAAAATTGAAAAGAGAT
  CATTTATTGTTACCAATAGATCATAATAAGGTACCTAATATTAAAAATTTAGATTCAGAT
  TATATGAATATGTCATTTGATAGAGGCAATAAATATTCTTTACCTTATTTCTTTGGAACT
  GTAGGTATTTTATATAATAAAGAAAAGTATCCAAATGAATCATTTGATAGTTGGAAGTCA
  TTGTATAATCCTAAATTTAAAAACCAAATTTTACTAGTTGACGGTGCTAGAGAGATTATA
  GGCATGAGTTTGAATAAACTTGGGTATAATCTTAATGACCGTAATTCGCACCATTTAAAA
  GAAGCAGAGCGAGATTTAACCAAACTAGCACCACAAGTAAGAGGTGTCGTAGGTGATGAA
  ATTACCATGATGCTTCAACAAAATGAAGGTAACATAGCGGTTGTTTGGAGTGGTGTTGCA
  GCACCTCTAGTGCAAGAAGGGGATAAATATAATTATGTTATTCCTAAAGAAGGATCGAAT
  TTATGGTTCGACAATATGGTAATTCCAAAAACGGCACAAAATAAAGAGGGTGCATATAAA
  TTTATGAATTTTTTATTAGATGCTAAAAATAACAAGCAAAATACAGAATTCGTAGGCTAT
  GCAACGCCAAACAAGGCTGCTCGACAATTGTTGCCTAAAGAGATTAAAGACGACCATCGT
  TTTTATCCGACTAAGAAAGAGCAAGAACGCCTTGAAGTTTATAAAGATTTAGGACCTGAA
  GTTTTAAGTGAATACAATGAAAACTTTTTGAATTTCAAAATGTCATTAAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS05425 [old locus tag: NWMN_0968 ]
• symbol: NWMN_RS05425
• description: spermidine/putrescine ABC transporter substrate-binding protein
• length: 357
• theoretical pI: 8.29147
• theoretical MW: 41471.1
• GRAVY: -0.583754

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 23.2)
  and 4 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter substrate-binding protein, CPR_0540 family (TIGR03850; HMM-score: 16.1)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 16)
  Metabolism Transport and binding proteins Other ABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 15.9)
  Metabolism Transport and binding proteins Other thiamin/thiamin pyrophosphate ABC transporter, thiamin/thiamin pyrophospate-binding protein (TIGR01276; HMM-score: 13.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PBP (CL0177) SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 123.8)
  SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 102.2)
  and 5 more
  SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 74.2)
  SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 53.4)
  Lipoprotein_8; Hypothetical lipoprotein (MG045 family) (PF02030; HMM-score: 33.1)
  ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 16.7)
  PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 33 aa
  • SP(Sec/SPI): 0.49937
  • TAT(Tat/SPI): 0.004282
  • LIPO(Sec/SPII): 0.241175
  • Cleavage Site: CS pos: 33-34. VHT-NQ. Pr: 0.1597
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446742434 NCBI
• RefSeq: WP_000819690 NCBI
• UniProt: see NWMN_0968

⊟Protein sequence

• MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGEYIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHLLLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKSLYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications