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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05435 [old locus tag: NWMN_0970 ]
  • pan locus tag?: SAUPAN003329000
  • symbol: NWMN_RS05435
  • pan gene symbol?: —
  • synonym:
  • product: membrane protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS05435 [old locus tag: NWMN_0970 ]
  • symbol: NWMN_RS05435
  • product: membrane protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1076290..1076709
  • length: 420
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1076290..1076709) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0970

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAATAGAAAACCTGAATTAATCATGGCTTGGATTGCAAATAGTATTAGTATTATTTAT
    TTATTAGTTATGGGGCTATCCTATTTTTCTTTAAAAAGTGGTAATGCATCACAACGTGAA
    GAATTAGCGAAGCAATTATCTCAGAACGGTGGCAAGGTTTCTTTAGATATGCTTCAGACA
    ACAATGGGTGCATTAGCAATTATTTTATTAATTTCAACACTTTATGGTATATTTGCGACA
    ATTTGTATTAAAGGACGTAGAAAATTATCGATTATACTTTTTGTTATCGCGATAATTGTA
    AGTTTGATGGCTCTTAATTTAATTGCAATTGTCTTATGGGTTATCGTGATGATTATGTTG
    ATTTCTAAAAAAGAATCAAAAGAAACAACACATAAGGACGATGAGTATATTTATCATTAA
    60
    120
    180
    240
    300
    360
    420

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS05435 [old locus tag: NWMN_0970 ]
  • symbol: NWMN_RS05435
  • description: membrane protein
  • length: 139
  • theoretical pI: 9.90462
  • theoretical MW: 15576.8
  • GRAVY: 0.682014

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides oligosaccharide repeat unit polymerase (TIGR04370; HMM-score: 15.2)
  • TheSEED: see NWMN_0970
  • PFAM:
    TSPAN_4TM-like (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 78.9)
    and 11 more
    no clan defined DUF6773; Family of unknown function (DUF6773) (PF20563; HMM-score: 18.8)
    DUF2663; Protein of unknown function (DUF2663) (PF10864; HMM-score: 15.9)
    DUF5993; Family of unknown function (DUF5993) (PF19455; HMM-score: 14.6)
    TSPAN_4TM-like (CL0347) DUF2569; Protein of unknown function (DUF2569) (PF10754; HMM-score: 11.4)
    no clan defined Tmemb_9; TMEM9 (PF05434; HMM-score: 11)
    P2X_receptor; ATP P2X receptor (PF00864; HMM-score: 10.2)
    EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 8.6)
    DUF7670; Domain of unknown function (DUF7670) (PF24709; HMM-score: 8.2)
    DUF4389; Domain of unknown function (DUF4389) (PF14333; HMM-score: 7.9)
    DHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 7.1)
    TSPAN_4TM-like (CL0347) Tetraspanin; Tetraspanin family (PF00335; HMM-score: 6.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.7841
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.2158
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.156691
    • TAT(Tat/SPI): 0.003661
    • LIPO(Sec/SPII): 0.031929
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAKQLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSLMALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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