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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06520 [old locus tag: NWMN_1156 ]
  • pan locus tag?: SAUPAN003538000
  • symbol: NWMN_RS06520
  • pan gene symbol?: sucD
  • synonym:
  • product: succinyl-CoA ligase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS06520 [old locus tag: NWMN_1156 ]
  • symbol: NWMN_RS06520
  • product: succinyl-CoA ligase subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1268031..1268939
  • length: 909
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1268031..1268939) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1156

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTATTTATAGATAAGAATACTAAAGTAATGGTACAAGGTATTACAGGGTCTACT
    GCCCTTTTCCATACAAAACAAATGCTTGATTATGGTACGAAAATAGTAGCAGGTGTGACG
    CCTGGTAAAGGTGGTCAAGTTGTTGAAGGCGTTCCTGTTTTCAACACTGTTGAAGAAGCT
    AAAAATGAAACTGGGGCAACGGTTTCAGTCATTTACGTTCCAGCACCATTTGCTGCAGAC
    TCAATTTTAGAAGCAGCTGATGCAGACTTAGATATGGTTATTTGTATCACTGAACATATT
    CCTGTATTAGACATGGTTAAAGTTAAACGCTACTTACAAGGTAGAAAAACACGTTTAGTT
    GGTCCGAACTGTCCAGGTGTGATTACAGCAGATGAATGTAAAATTGGTATTATGCCTGGC
    TATATTCACAAAAAAGGTCATGTTGGTGTAGTATCTCGTTCAGGTACATTAACATATGAA
    GCAGTGCACCAATTGACTGAAGAAGGTATTGGTCAAACTACAGCTGTTGGTATTGGTGGA
    GACCCAGTCAACGGAACAAACTTTATTGATGTTTTAAAAGCATTCAATGAAGATGACGAA
    ACGAAAGCAGTTGTTATGATTGGTGAAATCGGTGGTACGGCTGAAGAAGAAGCAGCTGAA
    TGGATTAAAGCGAATATGACAAAACCAGTTGTAGGCTTTATCGGTGGACAAACAGCACCT
    CCTGGAAAACGTATGGGACATGCTGGTGCAATCATTTCAGGTGGTAAAGGTACTGCTGAA
    GAGAAAATTAAAACATTAAATAGTTGTGGTGTGAAAACAGCGGCAACACCTTCAGAAATT
    GGTTCAACATTAATTGAAGCTGCTAAAGAAGCAGGTATTTATGAATCATTATTAACTGTT
    AATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    909

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS06520 [old locus tag: NWMN_1156 ]
  • symbol: NWMN_RS06520
  • description: succinyl-CoA ligase subunit alpha
  • length: 302
  • theoretical pI: 5.40845
  • theoretical MW: 31542
  • GRAVY: 0.0354305

⊟Function[edit | edit source]

  • reaction:
    EC 6.2.1.5?  ExPASy
    Succinate--CoA ligase (ADP-forming) ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle succinate-CoA ligase, alpha subunit (TIGR01019; EC 6.2.1.-; HMM-score: 479.4)
    and 1 more
    acetyl coenzyme A synthetase (ADP forming), alpha domain (TIGR02717; EC 6.2.1.13; HMM-score: 64.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) CoA_binding; CoA binding domain (PF02629; HMM-score: 102.9)
    and 3 more
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 68.3)
    Succ_CoA_lig; Succinyl-CoA ligase like flavodoxin domain (PF13607; HMM-score: 35.2)
    NADP_Rossmann (CL0063) CoA_binding_2; CoA binding domain (PF13380; HMM-score: 22.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018856
    • TAT(Tat/SPI): 0.002425
    • LIPO(Sec/SPII): 0.002333
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKGGQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEAAEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAATPSEIGSTLIEAAKEAGIYESLLTVNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_RS11780(deoA)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    NWMN_RS00315peptidoglycan-binding protein LysM  [1] (data from MRSA252)
    NWMN_RS00980L-lactate dehydrogenase  [1] (data from MRSA252)
    NWMN_RS0291050S ribosomal protein L11  [1] (data from MRSA252)
    NWMN_RS0292050S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_RS0292550S ribosomal protein L7/L12  [1] (data from MRSA252)
    NWMN_RS02960elongation factor G  [1] (data from MRSA252)
    NWMN_RS02965elongation factor Tu  [1] (data from MRSA252)
    NWMN_RS04195aldehyde dehydrogenase  [1] (data from MRSA252)
    NWMN_RS04590NADH dehydrogenase  [1] (data from MRSA252)
    NWMN_RS04730hypothetical protein  [1] (data from MRSA252)
    NWMN_RS05380pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    NWMN_RS05385pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    NWMN_RS05390dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)
    NWMN_RS05395dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    NWMN_RS0649050S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_RS0657530S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_RS07455dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    NWMN_RS07785DNA-binding protein HU  [1] (data from MRSA252)
    NWMN_RS08655Holliday junction branch migration DNA helicase RuvB  [1] (data from MRSA252)
    NWMN_RS0868550S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_RS08905isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    NWMN_RS08930pyruvate kinase  [1] (data from MRSA252)
    NWMN_RS12080Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    NWMN_RS1226030S ribosomal protein S9  [1] (data from MRSA252)
    NWMN_RS1230530S ribosomal protein S13  [1] (data from MRSA252)
    NWMN_RS1233050S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_RS1234030S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_RS1236550S ribosomal protein L5  [1] (data from MRSA252)
    NWMN_RS1239530S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_RS1241050S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_RS12590molybdate ABC transporter substrate-binding protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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