PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS06965 [old locus tag: NWMN_1233 ]
• pan locus tag^?: SAUPAN003689000
• symbol: NWMN_RS06965
• pan gene symbol^?: —
• synonym:
• product: two-component sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS06965 [old locus tag: NWMN_1233 ]
• symbol: NWMN_RS06965
• product: two-component sensor histidine kinase
• replicon: chromosome
• strand: +
• coordinates: 1357182..1358273
• length: 1092
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1357182..1358273) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1233

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
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  ATGAAATTTTTAAAAGATACTTCAATTGCTGAAATATCGTCTATACTTTATCTGATTTTT
  CCTATTGCCGGTATATTTTTTAATGAAGTATATGGTCCCAAATGGTTGTATATTATATCA
  GTCATTGTCTTTTCGTTGTCGTATCTTATATTAGTTATAGTAAATAATAGACTTAATACA
  TTAATGTTTTACATTTTGTTGATTATTCATTATTTTATTATTTGTTATTTTGTTTTCAGT
  GTACATCCAATGCTAAGTTTGTTTTTCTTTTATAGTGCTTTTGCCGTTCCATTTACTTTT
  AAAAATAATGTTAAAAAAACGGCAACTAATCTTTTCATACTAACAATGATTATATGTACA
  ATAATAACGTACTTATTGTATAACAACTATTTTGTTGCAATGATGGTTTATTATGTCGTT
  ATATCGTTAATAATGCTAGATAATTTTAAAAAAATGAAAAACCGTGAATATCAAAAAGAA
  ATAGCAGAAAAAAATAGACATATTAATACATTAATTGCTGAACAAGAGCGACATAGAATT
  GGTCAAGACTTACATGATACGTTAGGGCATGTGTTTGCAAGTTTATCATTAAAATCAGAA
  TTAGCTTATAAACTAATAGATGCTGATGTAGAAAAAGTAAAAGCTGAATTATTAGCAATT
  AATAAATTATCTCGTGAATCATTGAACAAAGTTCGAGAAATTATTGATGATGTAAAATTA
  CCATCATTTATTGAAGAGATTGATAGTATACGTAAAGTTTTAAAAGATGCTGATATTGAT
  TTTACATTTGAAAATAAAGAATTAGCGCAAGTATTAAGTCCTACTAAACAATCTATGTTA
  GTTATGATTACGCGTGAAGCGATAAATAATGTTATTAAACATGCAAATGCTTCAAAAGTT
  CATGGTAAATTAAAAACTGTAAACAATCATAAATTACTGCTTATGATTGAAGATGATGGC
  AAAGGTATCGATAGTGATTGTGAGGTGAAAAGTATTTCACAGCGTGTACAACATTTAAAT
  GGAACTTTAGCAGTCGACTCAACAAATGGAACTAAAATAATCATTGAAATCTCAACAGGA
  GGAATAGCATGA
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  1092
  

⊟Protein

⊟General

• locus tag: NWMN_RS06965 [old locus tag: NWMN_1233 ]
• symbol: NWMN_RS06965
• description: two-component sensor histidine kinase
• length: 363
• theoretical pI: 8.66465
• theoretical MW: 41630.8
• GRAVY: 0.293939

⊟Function

• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.3)
  and 1 more
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 8.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 61.3)
  and 6 more
  HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 21.7)
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.2)
  HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 13.6)
  P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.8)
  Peroxisome (CL0484) Pex24p; Integral peroxisomal membrane peroxin (PF06398; HMM-score: 10.2)
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 6.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001122
  • TAT(Tat/SPI): 0.000089
  • LIPO(Sec/SPII): 0.009412
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 446592954 NCBI
• RefSeq: WP_000670300 NCBI
• UniProt: see NWMN_1233

⊟Protein sequence

• MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVFSLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKNNVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELLAINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHLNGTLAVDSTNGTKIIIEISTGGIA

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications