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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1354 [new locus tag: SACOL_RS06895 ]
  • pan locus tag?: SAUPAN003689000
  • symbol: SACOL1354
  • pan gene symbol?: —
  • synonym:
  • product: sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1354 [new locus tag: SACOL_RS06895 ]
  • symbol: SACOL1354
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1361000..1362091
  • length: 1092
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAATTTTTAAAAGATACTTCAATTGCTGAAATATCGTCTATACTTTATCTGATTTTT
    CCTATTGCCGGTATATTTTTTAATGAAGTATATGGTCCCAAATGGTTGTATATTATATCA
    GTCATTGTCTTTTCGTTGTCGTATCTTATATTAGTTATAGTAAATAATAGACTTAATACA
    TTAATGTTTTACATTTTGTTGATTATTCATTATTTTATTATTTGTTATTTTGTTTTCAGT
    GTACATCCAATGCTAAGTTTGTTTTTCTTTTATAGTGCTTTTGCCGTTCCATTTACTTTT
    AAAAATAATGTTAAAAAAACGGCAACTAATCTTTTCATACTAACAATGATTATATGTACA
    ATAATAACGTACTTATTGTATAACAACTATTTTGTTGCAATGATGGTTTATTATGTCGTT
    ATATCGTTAATAATGCTAGATAATTTTAAAAAAATGAAAAACCGTGAATATCAAAAAGAA
    ATAGCAGAAAAAAATAGACATATTAATACATTAATTGCTGAACAAGAGCGACATAGAATT
    GGTCAAGACTTACATGATACGTTAGGGCATGTGTTTGCAAGTTTATCATTAAAATCAGAA
    TTAGCTTATAAACTAATAGATGCTGATGTAGAAAAAGTAAAAGCTGAATTATTAGCAATT
    AATAAATTATCTCGTGAATCATTGAACAAAGTTCGAGAAATTATTGATGATGTAAAATTA
    CCATCATTTATTGAAGAGATTGATAGTATACGTAAAGTTTTAAAAGATGCTGATATTGAT
    TTTACATTTGAAAATAAAGAATTAGCGCAAGTATTAAGTCCTACTAAACAATCTATGTTA
    GTTATGATTACGCGTGAAGCGATAAATAATGTTATTAAACATGCAAATGCTTCAAAAGTT
    CATGGTAAATTAAAAACTGTAAACAATCATAAATTACTGCTTATGATTGAAGATGATGGC
    AAAGGTATCGATAGTGATTGTGAGGTGAAAAGTATTTCACAGCGTGTACAACATTTAAAT
    GGAACTTTAGCAGTCGACTCAACAAATGGAACTAAAATAATCATTGAAATCTCAACAGGA
    GGAATAGCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1354 [new locus tag: SACOL_RS06895 ]
  • symbol: SACOL1354
  • description: sensor histidine kinase
  • length: 363
  • theoretical pI: 8.66465
  • theoretical MW: 41630.8
  • GRAVY: 0.293939

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.3)
    and 1 more
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 8.5)
  • TheSEED  :
    • sensor histidine kinase
  • PFAM:
    His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 61.3)
    and 6 more
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 21.7)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.2)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 13.6)
    P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.8)
    Peroxisome (CL0484) Pex24p; Integral peroxisomal membrane peroxin (PF06398; HMM-score: 10.2)
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 6.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001122
    • TAT(Tat/SPI): 0.000089
    • LIPO(Sec/SPII): 0.009412
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVFSLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKNNVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELLAINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHLNGTLAVDSTNGTKIIIEISTGGIA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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