PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1158 [new locus tag: SA_RS06575 ]
• pan locus tag^?: SAUPAN003689000
• symbol: SA1158
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1158 [new locus tag: SA_RS06575 ]
• symbol: SA1158
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1319297..1320388
• length: 1092
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123993 NCBI
• RefSeq: NP_374435 NCBI
• BioCyc: see SA_RS06575
• MicrobesOnline: 103461 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  121
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  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  
  ATGAAATTTTTAAAAGATACTTCAATTGCTGAAATATCGTCTATACTTTATCTGATTTTT
  CCTATTGCCGGTATATTTTTTAATGAAGTATATGGTCCCAAATGGTTGTATATTATATCA
  GTCATTGTCTTTTCGTTGTCGTATCTTATATTAGTTATAGTAAATAATAGACTTAATACA
  TTAATGTTTTACATTTTGTTGATTATTCATTATTTTATTATTTGTTATTTTGTTTTCAGT
  GTACATCCAATGCTAAGTTTGTTTTTCTTTTATAGTGCTTTTGCCGTTCCATTTACTTTT
  AAAAATAATGTTAAAAAAACGGCAACTAATCTTTTCATACTAACAATGATTATATGTACA
  ATAATAACGTACTTATTGTATAACAACTATTTTGTTGCAATGATGGTTTATTATGTCGTT
  ATATCGTTAATAATGCTAGATAATTTTAAAAAAATGAAAAACCGTGAATATCAAAAAGAA
  ATAGCAGAAAAAAATAGACATATTAATACATTAATTGCTGAACAAGAGCGACATAGAATT
  GGTCAAGACTTACATGATACGTTAGGGCATGTGTTTGCAAGTTTATCATTAAAATCAGAA
  TTAGCTTATAAACTAATAGATACTGATGTAGAAAAAGTAAAAGCTGAATTATTAGCAATT
  AATAAATTATCTCGTGAATCATTGAACAAAGTTCGAGAAATTATTGATGATGTAAAATTA
  CCATCATTTATTGAAGAGATTGATAGTATACGTAAAGTTTTAAAAGATGCTGATATTGAT
  TTTACATTTGAAAATAAAGAATTAGCGCAAGTATTAAGTCCTACTAAACAATCTATGTTA
  GTTATGATTACGCGTGAAGCGATAAATAATGTTATTAAACATGCAAATGCTTCAAAAGTT
  CATGGTAAATTAAAAACTGTAAACAATCATAAATTACTGCTTATGATTGAAGATGATGGC
  AAAGGTATCGATAGTGATTGTGAGGTGAAAAGTATTTCACAGCGTGTACAACATTTAAAT
  GGAACTTTAGCAGTCGACTCAACAAATGGAACTAAAATAATCATTGAAATCTCAACAGGA
  GGAATAGCATGA
  60
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  1080
  1092
  

⊟Protein

⊟General

• locus tag: SA1158 [new locus tag: SA_RS06575 ]
• symbol: SA1158
• description: hypothetical protein
• length: 363
• theoretical pI: 8.66465
• theoretical MW: 41660.8
• GRAVY: 0.287052

⊟Function

• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.2)
  and 1 more
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 8.5)
• TheSEED  :
  • Two-component system sensor histidine kinase
• PFAM:
  His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 61.6)
  and 6 more
  HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 21.7)
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.2)
  HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 13.4)
  P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.7)
  Peroxisome (CL0484) Pex24p; Integral peroxisomal membrane peroxin (PF06398; HMM-score: 10.6)
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 6.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001122
  • TAT(Tat/SPI): 0.000089
  • LIPO(Sec/SPII): 0.009412
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 15926902 NCBI
• RefSeq: NP_374435 NCBI
• UniProt: A0A0H3JU19 UniProt

⊟Protein sequence

• MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVFSLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKNNVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDTDVEKVKAELLAINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHLNGTLAVDSTNGTKIIIEISTGGIA

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1156 > SA1157 > SA1158 > SA1159

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications