PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS08345 [old locus tag: NWMN_1482 ]
• pan locus tag^?: SAUPAN004162000
• symbol: NWMN_RS08345
• pan gene symbol^?: dnaJ
• synonym:
• product: molecular chaperone DnaJ

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08345 [old locus tag: NWMN_1482 ]
• symbol: NWMN_RS08345
• product: molecular chaperone DnaJ
• replicon: chromosome
• strand: -
• coordinates: 1648137..1649276
• length: 1140
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1648137..1649276) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1482

⊟Phenotype

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⊟DNA sequence

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  GTGGCCAAAAGAGATTATTATGAAGTTTTAGGCATTAGTAAGGATGCTTCAAAAGATGAA
  ATCAAAAAAGCGTATCGAAAGCTTTCGAAAAAATATCATCCAGATATTAACAAAGAAGAA
  GGTGCAGATGAAAAGTTTAAAGAAATTTCTGAAGCCTATGAAGTTTTAAGTGATGATAAT
  AAACGCGCAAGCTATGATCAATTTGGACATGATGGTCCACAAGGTTTTGGTGGACAAGGA
  TTCAATGGCTCTGACTTTGGCGGTTTTAGTGGCTTCGGTGGCGGCGGTTTTGAAGATATC
  TTTAGCTCTTTCTTCGGTGGCGGCAGACAAAGAGATCCAAATGCACCGCAAAAAGGTGAT
  GATCTTCAGTATACAATGACATTAACATTTGAAGAAGCGGTATTTGGTACAACAAAAGAA
  ATTTCAATCCGTAAAGATGTAACATGCGAAACATGTCATGGTGATGGTGCAAAGCCTGGA
  ACAAGTAAAAAGACTTGTAGTTACTGTAATGGCGCTGGTCATGTAGCTGTAGAACAAAAC
  ACAATTTTAGGTAGAGTTCGTACTGAACAAGTTTGTCCTAAATGTAATGGAAGTGGTCAA
  GAATTTGAAGAAGCTTGTCCAACATGTCACGGAAAAGGTACTGAAAATAAAACAGTTAAA
  TTAGAAGTGAAAGTACCTGAAGGTGTCGACAATGAACAACAAATTAGATTAGCTGGTGAA
  GGCTCTCCAGGCGTGAATGGAGGACCTGCTGGTGATTTATACGTAGTATTTAGAGTTAAA
  CCATCTGAAACATTTAAACGTGATGGTGATGATATTTATTACAAATTAAATGTTAGCTTC
  CCACAAGCAGCTTTAGGCGATGAAATAAAAATACCAACATTAAATAATGAAGTTATGTTA
  ACAATCCCTGCTGGTACACAAACAGGTAAGCAATTCCGCTTAAAAGAAAAGGGTATAAAA
  AATGTTCATGGCTATGGATACGGAGATTTATATGTTGATATTAAAGTAGTAACGCCTACT
  AAATTAACAGATAGACAAAAAGAACTAATGAAAGAATTTGCCCAATTAAATGGTGAAGAA
  ATTAATGAACAACCTTCGAATTTTAAAGATAGAGCAAAAAGATTCTTTAAGGGAGAATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS08345 [old locus tag: NWMN_1482 ]
• symbol: NWMN_RS08345
• description: molecular chaperone DnaJ
• length: 379
• theoretical pI: 5.79
• theoretical MW: 41760.2
• GRAVY: -0.783641

⊟Function

• TIGRFAM:
  chaperone protein DnaJ (TIGR02349; HMM-score: 466)
  and 4 more
  Cellular processes Cellular processes Chemotaxis and motility terminal organelle assembly protein TopJ (TIGR03835; HMM-score: 157)
  Genetic information processing Protein fate Protein folding and stabilization Fe-S protein assembly co-chaperone HscB (TIGR00714; HMM-score: 21.4)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions uncharacterized phage protein (TIGR02642; HMM-score: 14.5)
  Genetic information processing Protein fate Protein modification and repair hydrogenase nickel insertion protein HypA (TIGR00100; HMM-score: 5.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined DnaJ_C; DnaJ C terminal domain (PF01556; HMM-score: 151.9)
  and 17 more
  Chaperone-J (CL0392) DnaJ; DnaJ domain (PF00226; HMM-score: 97.3)
  no clan defined DnaJ_CXXCXGXG; DnaJ central domain (PF00684; HMM-score: 51.2)
  C2H2-zf (CL0361) zf-C2H2_10; C2H2 zinc-finger (PF16588; HMM-score: 20.2)
  no clan defined Anti-TRAP; Tryptophan RNA-binding attenuator protein inhibitory protein (PF15777; HMM-score: 19)
  Cytochrome-c (CL0318) CCP_MauG; Di-haem cytochrome c peroxidase (PF03150; HMM-score: 13.9)
  Multiheme_cytos (CL0317) Cytochrome_C7; Cytochrome c7 and related cytochrome c (PF14522; HMM-score: 13.7)
  no clan defined DUF3365; Protein of unknown function (DUF3365) (PF11845; HMM-score: 12.9)
  Multiheme_cytos (CL0317) Cytochrome_C554; Cytochrome c554 and c-prime (PF13435; HMM-score: 12)
  Cytochrom_c3_2; Cytochrome c3 (PF14537; HMM-score: 11.9)
  no clan defined DUF5351; Family of unknown function (DUF5351) (PF17302; HMM-score: 11.9)
  Cytochrome-c (CL0318) Cytochrome_CBB3; Cytochrome C oxidase, cbb3-type, subunit III (PF13442; HMM-score: 10.9)
  RING (CL0229) zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger) (PF13920; HMM-score: 10.4)
  no clan defined HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 9.2)
  Cytochrome-c (CL0318) DUF1924; Domain of unknown function (DUF1924) (PF09086; HMM-score: 9.2)
  Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 9)
  RING (CL0229) zf-RING_10; zinc RING finger of MSL2 (PF16685; HMM-score: 6.9)
  no clan defined zf-CHY; CHY zinc finger (PF05495; HMM-score: 6.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007689
  • TAT(Tat/SPI): 0.001249
  • LIPO(Sec/SPII): 0.00262
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447041765 NCBI
• RefSeq: WP_001119021 NCBI
• UniProt: see NWMN_1482

⊟Protein sequence

• MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADEKFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFSSFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPGTSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKTVKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKLNVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDIKVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: HrcA see NWMN_1482

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications