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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01682
  • pan locus tag?: SAUPAN004162000
  • symbol: SAOUHSC_01682
  • pan gene symbol?: dnaJ
  • synonym:
  • product: chaperone protein DnaJ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01682
  • symbol: SAOUHSC_01682
  • product: chaperone protein DnaJ
  • replicon: chromosome
  • strand: -
  • coordinates: 1591338..1592477
  • length: 1140
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    GTGGCCAAAAGAGATTATTATGAAGTTTTAGGCATTAGTAAGGATGCTTCAAAAGATGAA
    ATCAAAAAAGCGTATCGAAAGCTTTCGAAAAAATATCATCCAGATATTAACAAAGAAGAA
    GGTGCAGATGAAAAGTTTAAAGAAATTTCTGAAGCCTATGAAGTTTTAAGTGATGATAAT
    AAACGCGCAAGCTATGATCAATTTGGACATGATGGTCCACAAGGTTTTGGTGGACAAGGA
    TTCAATGGCTCTGACTTTGGCGGTTTTAGTGGCTTCGGTGGCGGCGGTTTTGAAGATATC
    TTTAGCTCTTTCTTCGGTGGCGGCAGACAAAGAGATCCAAATGCACCGCAAAAAGGTGAT
    GATCTTCAGTATACAATGACATTAACATTTGAAGAAGCGGTATTTGGTACAACAAAAGAA
    ATTTCAATCCGTAAAGATGTAACATGCGAAACATGTCATGGTGATGGTGCAAAGCCTGGA
    ACAAGTAAAAAGACTTGTAGTTACTGTAATGGCGCTGGTCATGTAGCTGTAGAACAAAAC
    ACAATTTTAGGTAGAGTTCGTACTGAACAAGTTTGTCCTAAATGTAATGGAAGTGGTCAA
    GAATTTGAAGAAGCTTGTCCAACATGTCACGGAAAAGGTACTGAAAATAAAACAGTTAAA
    TTAGAAGTGAAAGTACCTGAAGGTGTCGACAATGAACAACAAATTAGATTAGCTGGTGAA
    GGCTCTCCAGGCGTGAATGGAGGACCTGCTGGTGATTTATACGTAGTATTTAGAGTTAAA
    CCATCTGAAACATTTAAACGTGATGGTGATGATATTTATTACAAATTAAATGTTAGCTTC
    CCACAAGCAGCTTTAGGCGATGAAATAAAAATACCAACATTAAATAATGAAGTTATGTTA
    ACAATCCCTGCTGGTACACAAACAGGTAAGCAATTCCGCTTAAAAGAAAAGGGTATAAAA
    AATGTTCATGGCTATGGATACGGAGATTTATATGTTGATATTAAAGTAGTAACGCCTACT
    AAATTAACAGATAGACAAAAAGAACTAATGAAAGAATTTGCCCAATTAAATGGTGAAGAA
    ATTAATGAACAACCTTCGAATTTTAAAGATAGAGCAAAAAGATTCTTTAAGGGAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01682
  • symbol: SAOUHSC_01682
  • description: chaperone protein DnaJ
  • length: 379
  • theoretical pI: 5.79
  • theoretical MW: 41760.2
  • GRAVY: -0.783641

⊟Function[edit | edit source]

  • TIGRFAM:
    chaperone protein DnaJ (TIGR02349; HMM-score: 466)
    and 4 more
    Cellular processes Cellular processes Chemotaxis and motility terminal organelle assembly protein TopJ (TIGR03835; HMM-score: 157)
    Genetic information processing Protein fate Protein folding and stabilization Fe-S protein assembly co-chaperone HscB (TIGR00714; HMM-score: 21.4)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions uncharacterized phage protein (TIGR02642; HMM-score: 14.5)
    Genetic information processing Protein fate Protein modification and repair hydrogenase nickel insertion protein HypA (TIGR00100; HMM-score: 5.7)
  • TheSEED  :
    • Chaperone protein DnaJ
    Protein Metabolism Protein folding Protein chaperones  Chaperone protein DnaJ
    and 1 more
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Chaperone protein DnaJ
  • PFAM:
    no clan defined DnaJ_C; DnaJ C terminal domain (PF01556; HMM-score: 151.9)
    and 17 more
    Chaperone-J (CL0392) DnaJ; DnaJ domain (PF00226; HMM-score: 97.3)
    no clan defined DnaJ_CXXCXGXG; DnaJ central domain (PF00684; HMM-score: 51.2)
    C2H2-zf (CL0361) zf-C2H2_10; C2H2 zinc-finger (PF16588; HMM-score: 20.2)
    no clan defined Anti-TRAP; Tryptophan RNA-binding attenuator protein inhibitory protein (PF15777; HMM-score: 19)
    Cytochrome-c (CL0318) CCP_MauG; Di-haem cytochrome c peroxidase (PF03150; HMM-score: 13.9)
    Multiheme_cytos (CL0317) Cytochrome_C7; Cytochrome c7 and related cytochrome c (PF14522; HMM-score: 13.7)
    no clan defined DUF3365; Protein of unknown function (DUF3365) (PF11845; HMM-score: 12.9)
    Multiheme_cytos (CL0317) Cytochrome_C554; Cytochrome c554 and c-prime (PF13435; HMM-score: 12)
    Cytochrom_c3_2; Cytochrome c3 (PF14537; HMM-score: 11.9)
    no clan defined DUF5351; Family of unknown function (DUF5351) (PF17302; HMM-score: 11.9)
    Cytochrome-c (CL0318) Cytochrome_CBB3; Cytochrome C oxidase, cbb3-type, subunit III (PF13442; HMM-score: 10.9)
    RING (CL0229) zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger) (PF13920; HMM-score: 10.4)
    no clan defined HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 9.2)
    Cytochrome-c (CL0318) DUF1924; Domain of unknown function (DUF1924) (PF09086; HMM-score: 9.2)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 9)
    RING (CL0229) zf-RING_10; zinc RING finger of MSL2 (PF16685; HMM-score: 6.9)
    no clan defined zf-CHY; CHY zinc finger (PF05495; HMM-score: 6.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007689
    • TAT(Tat/SPI): 0.001249
    • LIPO(Sec/SPII): 0.00262
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADEKFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFSSFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPGTSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKTVKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKLNVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDIKVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CtsR* (repression) regulon, HrcA* (repression) regulon
    CtsR*(TF)important in Heat shock response; transcription unit transferred from N315 data RegPrecise  [4]
    HrcA*(TF)important in Heat shock response; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  5. ↑ Arnaud Chastanet, Juliette Fert, Tarek Msadek
    Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria.
    Mol Microbiol: 2003, 47(4);1061-73
    [PubMed:12581359] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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