PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS08590 [old locus tag: NWMN_1530 ]
• pan locus tag^?: SAUPAN004231000
• symbol: NWMN_RS08590
• pan gene symbol^?: tcdA
• synonym:
• product: tRNA threonylcarbamoyladenosine dehydratase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08590 [old locus tag: NWMN_1530 ]
• symbol: NWMN_RS08590
• product: tRNA threonylcarbamoyladenosine dehydratase
• replicon: chromosome
• strand: -
• coordinates: 1694873..1695646
• length: 774
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1694873..1695646) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1530

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  ATGAAACATCAATTTTCAAGAAATGAACTAGCGATTGGACAACAAGGTTTAGATTTATTG
  AAGCAACAAACTGTTGTCATACTTGGCGTTGGAGGTGTTGGTTCATTTGCTGCTGAAGCA
  TTAGCAAGAACGAATATTGGTCATATTATATTAATAGATAAAGATGATGTTGATATTACA
  AATGTAAACAGACAATTACACGCTTTAACTACAACGGTAGGACAAAGTAAAGTTTCTTTG
  ATGGAAGAACGTATTAAATTAATTAATCCAGATTGTAAAGTCACGCCCTTACATATGTTT
  TATACAGAAGAGACATATGAAGAAATTTTCGATAACTACGATATAGATTATTTTGTAGAT
  GCAAGTGATACAATAATGTACAAAGTACACTTAATGAAAGAGTGTCTTGACCGAGGGATT
  AAAGTGATTTCCAGTATGGGTGCTGCAAATAAAACAGATCCAACACGTTTTACAATTTCA
  GATATCTCTAAAACACATACCGATCCAATGGCCAAAATTATTCGTCAAAAATTGAAAAAA
  CTAGGAATTCGTAAAGGGATTCCAGTTGTATTTTCAGATGAAAGTCCAATTGTCATAAGA
  GAAGATGTAAAAGATATAGTTGGAGATAAAAATGCTATCAATCGAAAAGGACAAATGCCA
  CCTTCTTCAAATGCCTTTGTGCCAAGTGTTGTTGGATTAATTTGTGCAAGTTATGTGGTG
  AATGACGTATTAAAAGATATTCCAGTTCGTCGCATTAAAGACAAAGGGCAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  774
  

⊟Protein

⊟General

• locus tag: NWMN_RS08590 [old locus tag: NWMN_1530 ]
• symbol: NWMN_RS08590
• description: tRNA threonylcarbamoyladenosine dehydratase
• length: 257
• theoretical pI: 8.07957
• theoretical MW: 28641
• GRAVY: -0.161479

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 84.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin synthase sulfurylase MoeB (TIGR02355; HMM-score: 77.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine biosynthesis protein ThiF (TIGR02354; HMM-score: 72.9)
  and 10 more
  PRTRC system ThiF family protein (TIGR03736; HMM-score: 24.1)
  ubiquitin-activating enzyme E1 (TIGR01408; HMM-score: 19.5)
  E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 18.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 17.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 16.1)
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)mycothiol dehydrogenase (TIGR03451; EC 1.1.1.306; HMM-score: 15.3)
  Genetic information processing Protein fate Protein folding and stabilization xanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 14.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other xanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 14.3)
  6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 13.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 12.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) ThiF; ThiF family (PF00899; HMM-score: 115.6)
  and 8 more
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 22.3)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 16.9)
  ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 16.5)
  2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 14.6)
  UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 14.4)
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 13.9)
  DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 12.3)
  Trp_halogenase; Tryptophan halogenase (PF04820; HMM-score: 10.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.236347
  • TAT(Tat/SPI): 0.292054
  • LIPO(Sec/SPII): 0.002753
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446605300 NCBI
• RefSeq: WP_000682646 NCBI
• UniProt: see NWMN_1530

⊟Protein sequence

• MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALARTNIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYTEETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTISDISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQMPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications