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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS10545 [old locus tag: NWMN_1835 ]
  • pan locus tag?: SAUPAN004921000
  • symbol: NWMN_RS10545
  • pan gene symbol?: rumA
  • synonym:
  • product: RNA methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS10545 [old locus tag: NWMN_1835 ]
  • symbol: NWMN_RS10545
  • product: RNA methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2041451..2042812
  • length: 1362
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2041451..2042812) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1835

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGCAAGCAATAGCTAAAAATGACATAAAAACGGGAACAGTTGTTGATTTAACGCATGAA
    GGCCATGGTGTCGTAAAAATAGATCGCTTTCCTATTTTCATACCACAAGCTTTAATTAAT
    GAACAAATTGAATATAAAATTATCAAAGTAAAAAAGAACTTTGCGATAGGTAAGTTGCTT
    AATATAAATACGAGAAGTGAGAATCGTGTTGCACCGCCATGTATATATTATGAACGTTGT
    GGTGGGTGCCAACTTCAACACTTATCATACGAAGCACAACTTGAAATGAAAAAGGAACAA
    GTGATTAATTTGTTTCAGCGAAAAGCGCATTTTGATAATTCTAAGATTAACGATACAGTT
    GGCATGACAGATCCTTGGAGATATCGAAACAAGTCCCAGATTCCAGTTGGTAAGAATGAA
    CAGAATGAAGTTATTATGGGCTTTTATCGTCAAAGAAGCCATGACATTATTGACATGGAA
    AGTTGTTTAATACAAGATTCACAACATCAAGAAGTGATGAATGAAGTTAAATCTATTTTA
    AAAGATTTAAATGTAAGTATTTATCAAGAACAATTGAAAAAAGGTTTAATGAGACATTTG
    GTTGTAAGAACAGGATATCATACAGACGAAATGATGATTATTTTTGTAACTAATGGTAAA
    AAGTGGCCACAAAAAAATGCTGTTGTTGAGAAGATATTAGATGCATTTCCTAATGTGACA
    AGCATTAAACAAAACATTAATGATAGCCACTCTAATGTTATTATGGGACGTCAATCTATA
    ACGCTATATGGTAAAGATACAATTATTGATCAATTAACAGATAGTACATTTAAAATAAGT
    GATCAGTCATTCTACCAAATTAATTCTGAACAAACAGAGAAATTATATAATAAAGCAATT
    GAGTATGCGCAATTGACAGGTAATGAAGTTGTATTAGATACCTATTGTGGTATTGGAACA
    ATAGGGCTATATATGGCACCACTTGCAAAACATGTATACGGTGTTGAAGTAGTACCTTCT
    GCAATTGAAGACGCACAACAAAATGCGACTATAAACCAATGCAATAATACAACTTTTGTC
    TGTGGGAAAGCTGAAGAAGTTATTTTACAATGGAAAGCACAAGGGATTAAACCAGATGTT
    GTAATGGTTGATCCGCCAAGAAAGGGCTGTGACGAAACGTTTATTCAGACTTTGCTTACA
    TTAGAACCTAAGCGTATTGTCTATATTTCATGTAATCCCGCAACACAGCAACGTGATGCA
    TTATTATTAGCTGAAAAATATCAACTTGAAGAGGTAACACCGGTAGATATGTTTCCACAA
    ACAACACATGTTGAGACAGTGGCATTATTCAATTTGAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1362

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS10545 [old locus tag: NWMN_1835 ]
  • symbol: NWMN_RS10545
  • description: RNA methyltransferase
  • length: 453
  • theoretical pI: 6.73139
  • theoretical MW: 51682
  • GRAVY: -0.34702

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 527.8)
    and 13 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumB (TIGR02085; EC 2.1.1.189; HMM-score: 173.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (uracil(54)-C(5))-methyltransferase (TIGR02143; EC 2.1.1.35; HMM-score: 84.7)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 32.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 22.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 21.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 21.2)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 20.1)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 18.7)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 17.4)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 15.1)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 13.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 13.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 13.5)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) tRNA_U5-meth_tr; tRNA (Uracil-5-)-methyltransferase (PF05958; HMM-score: 84.9)
    and 14 more
    Met_10; Met-10+ like-protein (PF02475; HMM-score: 32.9)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 31.2)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 30.1)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 28.9)
    Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 26)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 22.1)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 22)
    N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 19.5)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 17.6)
    Cystine-knot (CL0079) PDGF; PDGF/VEGF domain (PF00341; HMM-score: 16.3)
    OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 15.2)
    NADP_Rossmann (CL0063) Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 14.9)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 13.1)
    TPMT; Thiopurine S-methyltransferase (TPMT) (PF05724; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001561
    • TAT(Tat/SPI): 0.000097
    • LIPO(Sec/SPII): 0.000479
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIEYKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVINLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDMESCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTNGKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDSTFKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYGVEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDETFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALFNLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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