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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02113
  • pan locus tag?: SAUPAN004921000
  • symbol: SAOUHSC_02113
  • pan gene symbol?: rumA
  • synonym:
  • product: RNA methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02113
  • symbol: SAOUHSC_02113
  • product: RNA methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1985147..1986508
  • length: 1362
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGCAAGCAATAGCTAAAAATGACATAAAAACGGGAACAGTTGTTGATTTAACGCATGAA
    GGCCATGGTGTCGTAAAAATAGATCGCTTTCCTATTTTCATACCACAAGCTTTAATTAAT
    GAACAAATTGAATATAAAATTATCAAAGTAAAAAAGAACTTTGCGATAGGTAAGTTGCTT
    AATATAAATACGAGAAGTGAGAATCGTGTTGCACCGCCATGTATATATTATGAACGTTGT
    GGTGGGTGCCAACTTCAACACTTATCATACGAAGCACAACTTGAAATGAAAAAGGAACAA
    GTGATTAATTTGTTTCAGCGAAAAGCGCATTTTGATAATTCTAAGATTAACGATACAGTT
    GGCATGACAGATCCTTGGAGATATCGAAACAAGTCCCAGATTCCAGTTGGTAAGAATGAA
    CAGAATGAAGTTATTATGGGCTTTTATCGTCAAAGAAGCCATGACATTATTGACATGGAA
    AGTTGTTTAATACAAGATTCACAACATCAAGAAGTGATGAATGAAGTTAAATCTATTTTA
    AAAGATTTAAATGTAAGTATTTATCAAGAACAATTGAAAAAAGGTTTAATGAGACATTTG
    GTTGTAAGAACAGGATATCATACAGACGAAATGATGATTATTTTTGTAACTAATGGTAAA
    AAGTGGCCACAAAAAAATGCTGTTGTTGAGAAGATATTAGATGCATTTCCTAATGTGACA
    AGCATTAAACAAAACATTAATGATAGCCACTCTAATGTTATTATGGGACGTCAATCTATA
    ACGCTATATGGTAAAGATACAATTATTGATCAATTAACAGATAGTACATTTAAAATAAGT
    GATCAGTCATTCTACCAAATTAATTCTGAACAAACAGAGAAATTATATAATAAAGCAATT
    GAGTATGCGCAATTGACAGGTAATGAAGTTGTATTAGATACCTATTGTGGTATTGGAACA
    ATAGGGCTATATATGGCACCACTTGCAAAACATGTATACGGTGTTGAAGTAGTACCTTCT
    GCAATTGAAGACGCACAACAAAATGCGACTATAAACCAATGCAATAATACAACTTTTGTC
    TGTGGGAAAGCTGAAGAAGTTATTTTACAATGGAAAGCACAAGGGATTAAACCAGATGTT
    GTAATGGTTGATCCGCCAAGAAAGGGCTGTGACGAAACGTTTATTCAGACTTTGCTTACA
    TTAGAACCTAAGCGTATTGTCTATATTTCATGTAATCCCGCAACACAGCAACGTGATGCA
    TTATTATTAGCTGAAAAATATCAACTTGAAGAGGTAACACCGGTAGATATGTTTCCACAA
    ACAACACATGTTGAGACAGTGGCATTATTCAATTTGAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1362

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02113
  • symbol: SAOUHSC_02113
  • description: RNA methyltransferase
  • length: 453
  • theoretical pI: 6.73139
  • theoretical MW: 51682
  • GRAVY: -0.34702

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 527.8)
    and 13 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumB (TIGR02085; EC 2.1.1.189; HMM-score: 173.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (uracil(54)-C(5))-methyltransferase (TIGR02143; EC 2.1.1.35; HMM-score: 84.7)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 32.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 22.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 21.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 21.2)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 20.1)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 18.7)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 17.4)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 15.1)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 13.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 13.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 13.5)
  • TheSEED  :
    • RNA methyltransferase, TrmA family
  • PFAM:
    NADP_Rossmann (CL0063) tRNA_U5-meth_tr; tRNA (Uracil-5-)-methyltransferase (PF05958; HMM-score: 84.9)
    and 14 more
    Met_10; Met-10+ like-protein (PF02475; HMM-score: 32.9)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 31.2)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 30.1)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 28.9)
    Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 26)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 22.1)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 22)
    N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 19.5)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 17.6)
    Cystine-knot (CL0079) PDGF; PDGF/VEGF domain (PF00341; HMM-score: 16.3)
    OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 15.2)
    NADP_Rossmann (CL0063) Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 14.9)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 13.1)
    TPMT; Thiopurine S-methyltransferase (TPMT) (PF05724; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001561
    • TAT(Tat/SPI): 0.000097
    • LIPO(Sec/SPII): 0.000479
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIEYKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVINLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDMESCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTNGKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDSTFKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYGVEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDETFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALFNLK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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