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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS10815 [old locus tag: NWMN_1881 ]
- pan locus tag?: SAUPAN005029000
- symbol: NWMN_RS10815
- pan gene symbol?: ami
- synonym:
- product: amidase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS10815 [old locus tag: NWMN_1881 ]
- symbol: NWMN_RS10815
- product: amidase
- replicon: chromosome
- strand: -
- coordinates: 2092679..2093434
- length: 756
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGAAAACATACAGTGAAGCAAGAGCAAGGTTACGTTGGTATCAAGGTAGATATATTGAT
TTTGACGGTTGGTATGGTTACCAATGTGCAGATTTAGCAGTTGATTACATTTATTGGTTG
TTAGAAATTAGAATGTGGGGAAATGCAAAAGATGCAATCAATAACGATTTTAAAAACATG
GCAACAGTATATGAAAACACACCATCGTTTGTTCCACAAATAGGTGATGTGGCTGTATTT
ACCAAAGGAATATATAAACAATACGGTCATATTGGTTTAGTGTTTAATGGTGGTAATACA
AATCAATTTTTAATTTTGGAACAGAACTATGACGGTAACGCAAATACGCCTGCAAAGTTA
CGTTGGGATAATTATTACGGCTGTACTCACTTTATTAGACCTAAGTATAAAAGTGAGGGC
TTAATGAATAAGATCACAAATAAAGTTAAACCACCTGCTCAAAAAGCAGTCGGTAAATCT
GCAAGTAAAATAACAGTTGGAAGTAAAGCGCCTTATAACCTTAAATGGTCAAAAGGTGCT
TATTTTAATGCGAAAATCGACGGCTTAGGTGCTACTTCAGCCACTAGATACGGTGATAAT
CGTACTAACTATAGATTCGATGTTGGACAGGCTGTATACGCGCCTGGAACATTAATATAT
GTGTTTGAAATTATAGATGGTTGGTGTCGCATTTATTGGAACAATCATAATGAGTGGATA
TGGCATGAGAGATTGATTGTGAAAGAAGTGTTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS10815 [old locus tag: NWMN_1881 ]
- symbol: NWMN_RS10815
- description: amidase
- length: 251
- theoretical pI: 9.52361
- theoretical MW: 29057.7
- GRAVY: -0.484861
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: data available for N315, NCTC8325, USA300_FPR3757
- PFAM: Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 45.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.022228
- TAT(Tat/SPI): 0.000344
- LIPO(Sec/SPII): 0.001201
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIRMWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQFLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKSASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTLIYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.