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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1923 [new locus tag: SAUSA300_RS10545 ]
  • pan locus tag?: SAUPAN005029000
  • symbol: SAUSA300_1923
  • pan gene symbol?: ami
  • synonym:
  • product: autolysin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1923 [new locus tag: SAUSA300_RS10545 ]
  • symbol: SAUSA300_1923
  • product: autolysin
  • replicon: chromosome
  • strand: -
  • coordinates: 2089107..2089862
  • length: 756
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAAACATACAGTGAAGCAAGAGCAAGGTTACGTTGGTATCAAGGTAGATATATTGAT
    TTTGACGGTTGGTATGGTTACCAATGTGCAGATTTAGCAGTTGATTACATTTATTGGTTG
    TTAGAAATTAGAATGTGGGGAAATGCAAAAGATGCAATCAATAACGATTTTAAAAACATG
    GCAACAGTATATGAAAACACACCATCGTTTGTTCCACAAATAGGTGATGTGGCTGTATTT
    ACCAAAGGAATATATAAACAATACGGTCATATTGGTTTAGTGTTTAATGGTGGTAATACA
    AACCAATTTTTAATTTTGGAACAGAACTATGACGGTAACGCAAATACGCCTGCAAAGTTA
    CGTTGGGATAATTATTACGGCTGTACTCACTTTATTAGACCTAAGTATAAAAGTGAGGGC
    TTAATGAATAAGATCACAAATAAAGTTAAACCACCTGCTCAAAAAGCAGTCGGTAAATCT
    GCAAGTAAAATAACAGTTGGAAGTAAAGCGCCTTATAACCTTAAATGGTCAAAAGGTGCT
    TATTTTAATGCGAAAATCGACGGCTTAGGTGCTACTTCAGCCACTAGATACGGTGATAAT
    CGTACTAACTATAGATTCGATGTTGGACAGGCTGTATACGCGCCTGGAACATTAATATAT
    GTGTTTGAAATTATAGATGGTTGGTGTCGCATTTATTGGAACAATCATAATGAGTGGATA
    TGGCATGAGAGATTGATTGTGAAAGAAGTGTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    756

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1923 [new locus tag: SAUSA300_RS10545 ]
  • symbol: SAUSA300_1923
  • description: autolysin
  • length: 251
  • theoretical pI: 9.52361
  • theoretical MW: 29057.7
  • GRAVY: -0.484861

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.28?  ExPASy
    N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
  • TIGRFAM:
  • TheSEED  :
    • Phage endolysin CHAP endopeptidase
  • PFAM:
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 45.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022228
    • TAT(Tat/SPI): 0.000344
    • LIPO(Sec/SPII): 0.001201
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIRMWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQFLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKSASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTLIYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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