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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1924 [new locus tag: SAUSA300_RS10550 ]
  • pan locus tag?: SAUPAN005030000
  • symbol: SAUSA300_1924
  • pan gene symbol?: —
  • synonym:
  • product: holin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1924 [new locus tag: SAUSA300_RS10550 ]
  • symbol: SAUSA300_1924
  • product: holin
  • replicon: chromosome
  • strand: -
  • coordinates: 2089874..2090128
  • length: 255
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    ATGATTAATTGGAAAATTAGAATGAAACAAAAATCATTTTGGGTAGCGATATTGTCAGCT
    ATCTTTTTATTTGCTCAAAACATCGCAAAAGCTATTGGGTATGATATCCAAGTTTATACA
    GAGCAATTAACAGACGGTTTAAACGCTATATTAGGATTTTTAGTATTAACTGGTGTGATT
    CAAGACCCGACTACTAAAGGTATAGGTGATAGCCACCAAGCTTTAGAATATGAAGAACCA
    AGAAGAAAATACTAG
    60
    120
    180
    240
    255

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1924 [new locus tag: SAUSA300_RS10550 ]
  • symbol: SAUSA300_1924
  • description: holin
  • length: 84
  • theoretical pI: 8.92064
  • theoretical MW: 9591.08
  • GRAVY: 0.0202381

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions holin, phage phi LC3 family (TIGR01598; HMM-score: 86.6)
  • TheSEED  :
    • Phage holin
  • PFAM:
    no clan defined Phage_holin_1; Bacteriophage holin (PF04531; HMM-score: 100.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: QNIAKAIG
  • SignalP: Signal peptide SP(Sec/SPI) length 31 aa
    • SP(Sec/SPI): 0.89249
    • TAT(Tat/SPI): 0.003745
    • LIPO(Sec/SPII): 0.00839
    • Cleavage Site: CS pos: 31-32. AKA-IG. Pr: 0.7707
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLTDGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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