PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS11110 [old locus tag: NWMN_1925 ]
• pan locus tag^?: SAUPAN005188000
• symbol: NWMN_RS11110
• pan gene symbol^?: int3
• synonym:
• product: site-specific integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS11110 [old locus tag: NWMN_1925 ]
• symbol: NWMN_RS11110
• product: site-specific integrase
• replicon: chromosome
• strand: +
• coordinates: 2131184..2132221
• length: 1038
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2131184..2132221) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1925

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAA
  AGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAG
  TTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTC
  GAAAATTGGATTAAAACATATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTAT
  AGAAATGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATA
  AATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACGCAAAAGAAACT
  ATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTT
  ATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAA
  CAAGAAAAATTCATCTCGGTAACTGAATATGAAATACTAAAAGATCACGTCAGAAAGAAG
  AGAACTCGTTCATCATTAGCGCTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGT
  GCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAG
  CGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATT
  ATGGACGTCATAAGTACATTTGCAATTAGCTATGATGGTTACATTTTCAAAGAAGGCGGA
  TCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAAT
  ATACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAA
  GGTGTATCTATACATTACATTTCTAAAAGATTAGGTCATAAAAATATAGCAATAACTACA
  TCTGTGTATTCTCATTTGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAA
  ATTTTAGAAAGTATGTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS11110 [old locus tag: NWMN_1925 ]
• symbol: NWMN_RS11110
• description: site-specific integrase
• length: 345
• theoretical pI: 10.2787
• theoretical MW: 40288.1
• GRAVY: -0.686667

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 70.5)
  and 2 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 44.1)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 44.1)
• TheSEED: data available for N315, NCTC8325, USA300_FPR3757
• PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 78)
  and 8 more
  l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 40.4)
  Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 36.3)
  MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 16.8)
  l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 16.2)
  no clan defined DUF1722; Protein of unknown function (DUF1722) (PF08349; HMM-score: 14.5)
  NblA; Phycobilisome degradation protein nblA (PF04485; HMM-score: 13.1)
  MACRO (CL0223) Macro; Macro domain (PF01661; HMM-score: 13)
  AEP (CL0243) Replicase; Replicase family (PF03090; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01857
  • TAT(Tat/SPI): 0.000569
  • LIPO(Sec/SPII): 0.004253
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446779935 NCBI
• RefSeq: WP_000857191 NCBI
• UniProt: see NWMN_1925

⊟Protein sequence

• MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNELRSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINRQVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSEQEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFIDERKTNTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEGGSIINLHAINNALKSACRVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEEDKKTTKILESM

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications