PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS11825 [old locus tag: NWMN_2048 ]
• pan locus tag^?: SAUPAN005442000
• symbol: NWMN_RS11825
• pan gene symbol^?: —
• synonym:
• product: NAD(P)-dependent oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS11825 [old locus tag: NWMN_2048 ]
• symbol: NWMN_RS11825
• product: NAD(P)-dependent oxidoreductase
• replicon: chromosome
• strand: -
• coordinates: 2265848..2266513
• length: 666
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2265848..2266513) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2048

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  661
  
  ATGAATATTTTGGTTATAGGTGCTAATGGCGGTGTAGGTTCGTTACTAGTGCAACAATTA
  GCAAAAGAAAATGTACCATTTACTGCTGGTGTTAGGCAATCAGATCAACTTAATGCGTTA
  AAATCACAAGGTATGAAAGCAATTCTTGTTGATGTTGAAAATGATTCAATAGAGACTTTA
  ACTGAGACGTTTAAACCATTCGATAAAGTTATCTTTTCAGTAGGTTCTGGAGGGAACACG
  GGTGCAGATAAAACAATTATTGTCGATTTAGATGGTGCTGTTAAATCAATGATTGCGAGT
  AAAGAGGCCAATATTAAACACTATGTCATGGTTTCAACATATGATTCGAGACGTCAGGCT
  TTTGATGATAGCGGTGATTTAAAGCCATATACAATAGCTAAACATTACGCTGATGACTAT
  TTAAGACGTTCAGGTTTGAATTATACAATTTTACATCCAGGGGCACTTACAAATGCAGCA
  GGATCTGGAAAGATAGAAGCTGCACAATATTTTGATGGTAAAGGTGAAATTCCTAGAGAA
  GATGTTGCGACAGTATTAAAAGAAATTGTCACATCCAATCATTTTAATCACCAAGAGTTT
  CAAATTATTAGTGGTGATCAGGACATTAAAGATGCTTTAACTCAATTTGAAAATGAAACG
  GATTAA
  60
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  420
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  540
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  660
  666
  

⊟Protein

⊟General

• locus tag: NWMN_RS11825 [old locus tag: NWMN_2048 ]
• symbol: NWMN_RS11825
• description: NAD(P)-dependent oxidoreductase
• length: 221
• theoretical pI: 4.70039
• theoretical MW: 24031.7
• GRAVY: -0.29638

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 21.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 18.9)
  and 9 more
  thioester reductase domain (TIGR01746; HMM-score: 16.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 14.8)
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 14.7)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 13.1)
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 12.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, substrate binding protein, PQQ-dependent alcohol dehydrogenase system (TIGR03863; HMM-score: 12.3)
  ergot alkaloid biosynthesis protein, AFUA_2G17970 family (TIGR03649; HMM-score: 12.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 11.9)
  crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 10.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 138.7)
  and 10 more
  NmrA; NmrA-like family (PF05368; HMM-score: 44.6)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 36.6)
  3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 20.8)
  ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 19.2)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 18.8)
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 16.1)
  DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 14.5)
  Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 13.7)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 13)
  NAD_binding_4; Male sterility protein (PF07993; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.021841
  • TAT(Tat/SPI): 0.001265
  • LIPO(Sec/SPII): 0.003331
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446946838 NCBI
• RefSeq: WP_001024094 NCBI
• UniProt: see NWMN_2048

⊟Protein sequence

• MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQGMKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKEANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAAGSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFENETD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB see NWMN_2048

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications