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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS11925 [old locus tag: NWMN_2066 ]
  • pan locus tag?: SAUPAN005511000
  • symbol: NWMN_RS11925
  • pan gene symbol?: tnp4
  • synonym:
  • product: ISL3 family transposase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS11925 [old locus tag: NWMN_2066 ]
  • symbol: NWMN_RS11925
  • product: ISL3 family transposase
  • replicon: chromosome
  • strand: +
  • coordinates: 2294933..2296135
  • length: 1203
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2294933..2296135) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2066

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTGTAATGATACCTTAGAATTACTAAGAATAAAAGATGAAAATATAAAATATATAAAC
    CAAGAAATTGACGTCATTATCAAAGGAAAAAAAGCAACAGTGGTTAATGCTGTACTAACG
    TATAAGCCTTCGGCCTGTTATTGTTGTGGAGTTAAAAATGAAGGACAAATTCATAAACAT
    GGTAAGCGTGTTTCTCGTATTACTTTACTTAAAACTCAAGGGTATAACACATACCTCAAC
    TTAGCTAAACAACGTTTTAAATGCTTAGAATGCAATGGCACTTTTACTGCTAAAACGTCA
    ATTGTTGATGAGTCGTGTTTTATCTCAAGATGTGTTACTCAAAAAGTTATAGAAGAAGCT
    ACTAAAGTTAAAACAGAGATTGATACTGCAGAAGATAACTGTATCTCTCCATCTACTGTA
    AGTCGTATTAGAACTAAAGCGGCTAATTCATTACGAATTAAACCCTTTAATTGTTTGCCA
    GAACACATCGCTATGGATGAATTTAAAAGCGTTAAAAATGTAACTGGATCAATGAGTTTC
    ATTTTTATAGATAATGATACTCATGATGTTATAGATATTTTAGAAAATAGAACTACAAGA
    TTCTTGCGTGCCTATTTCGAGCGATTCGATTTAAAAAATCGACAACAAGTTAAGACGGTT
    ACTATTGACATGTATGAACCCTATGTCCGATTATTTCGCGACCTATTTCCTAATGCAGCT
    ATTATTTTTGACAGATTCCATATCGTTCAACATTTAAATAGAGAACTTAATAAGTATCGT
    GTACAAGTTATGAATGAATACCGTAATAAAAAAGGACCTGATTATACAATTTTTAAGAAT
    AACTGGAAAGTCCTATTGATGGATACTAGTAAAACCATATTTAGTAAATACAGATGGAAT
    AAATCTTTTAAGGCTTATAAACGCTCATCTGACATTGTAGAATTCATGCTTTCAAAAGAC
    GATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGT
    AATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGT
    GTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTAT
    TACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAG
    TGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS11925 [old locus tag: NWMN_2066 ]
  • symbol: NWMN_RS11925
  • description: ISL3 family transposase
  • length: 400
  • theoretical pI: 10.2086
  • theoretical MW: 47014.3
  • GRAVY: -0.46875

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.9)
    Metabolism Central intermediary metabolism One-carbon metabolism S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.9)
  • TheSEED: data available for COL, NCTC8325, USA300_FPR3757
  • PFAM:
    RNase_H (CL0219) DDE_Tnp_ISL3; Transposase (PF01610; HMM-score: 145)
    and 4 more
    Zn_Beta_Ribbon (CL0167) zf-ISL3; zinc-finger of transposase IS204/IS1001/IS1096/IS1165 (PF14690; HMM-score: 42.6)
    HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20.8)
    no clan defined DUF3795; Protein of unknown function (DUF3795) (PF12675; HMM-score: 10.4)
    DUF4163; Domain of unknown function (DUF4163) (PF13739; HMM-score: 9.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.179974
    • TAT(Tat/SPI): 0.017784
    • LIPO(Sec/SPII): 0.012804
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPSACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIVDESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLPEHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVKTVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYTIFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLRKDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGINNKIKLIK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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