PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS12510 [old locus tag: NWMN_2166 ]
• pan locus tag^?: SAUPAN005723000
• symbol: NWMN_RS12510
• pan gene symbol^?: —
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS12510 [old locus tag: NWMN_2166 ]
• symbol: NWMN_RS12510
• product: MFS transporter
• replicon: chromosome
• strand: +
• coordinates: 2391418..2392629
• length: 1212
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2391418..2392629) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2166

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
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  301
  361
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  481
  541
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  1021
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  1141
  1201
  
  ATGACTAATCAAGGCTCATTAAATAAATCACCTATTTTCACCAAAAGTTTTACAATTAAC
  TTTTTAGTCAACTTTATCGTATACCTATGCATGTATTTGTTACTTGTAGTTATTGCTGGC
  TATAGTAAGCAAGCTTTCGACGCTTCCGATTCATTAGCCGGATTAGTTGTAGGACTATTT
  ATTGTCGGGTCTTTAATTGGTCGATTTGCTACAGGTAAATTTGTAAATCAAATCGGACCT
  AAACGATTATTATTTATTGGTTTAATTGCTTTAATTATCACGCAATTACTTTATTTTATT
  GATGGATCTTTAGCTTTTTTAATCTTTGTACGTCTCATAAACGGTATCGCAACTGCAGTT
  GTGACAACTGCAACAGGTACTATTGCGGCATACGTTACCCCTGTTAATAGAAAAAGTGAA
  GGCATCAGTTTATTTTCCTTAAGTTTAGTATTAGGTACAGCGATTGGCCCATTTCTAGGC
  ATGTTACTTATTACAAAATACGCAATTGACTTACTGTTTATTATTTGTGTCATATTAGGC
  ATACTCGGACTCATTATCTCATTATTTATAAAAGTTGATTTCGAAGTAACAAATAACAAA
  ACTGAAACTAATGTCATTGATAAACCAAGATTTAGTATTCATCAATTTATTGCAAAAGAG
  GCCATTCCTGTTGCTGTAATTATGCTTTTAATTGGCGTTACCTATGCGTCCATCCTAACT
  TATTTACAAGCATTTGCTTTAGAGCGACACCTAGTCACTGCAGCAAGCTATTTCTTTATT
  TGCTATGCTATAGCATCGTTAATTACTAGACCTATTGCTGGTAGATTGATGGATGATAAA
  AATGAAAATATTATTGTCTACCCAGCATTTATCATGCTTTTCCTTTCATTTGTATGCTTA
  ATTTCAAGCTATCAAAGCTGGTTAATATTACTTGCTGGTGCTTGCCTAGGTTTAGGGTAT
  GGAAATTTATCATCTGCAATGCAATCTATTGCGATAAAAGTCTCACCCCCGATTAAATAT
  GGTATCGCGACATCTACTTTTTACGTTGGTCTAGATGCAGGTGTCGGCTTCGGTCCATCA
  TTCCTTGGGTTATTTACTCATATGTTTTCATATAGTGAAATCTTCGGCTTTATGGCCGCT
  TTAGCAATCATTACTATGCTTGTTTACTTCTTAATTCATGGACGTCATGTAACTAGAAAT
  GCCATGAACTAA
  60
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  1212
  

⊟Protein

⊟General

• locus tag: NWMN_RS12510 [old locus tag: NWMN_2166 ]
• symbol: NWMN_RS12510
• description: MFS transporter
• length: 403
• theoretical pI: 9.3132
• theoretical MW: 43887.2
• GRAVY: 1.00422

⊟Function

• TIGRFAM:
  multidrug resistance protein (TIGR00880; HMM-score: 72.5)
  and 11 more
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 54.6)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 54.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 45.3)
  Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 34.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sugar porter (SP) family (TIGR00879; HMM-score: 29.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 29.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 29.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 28.2)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 27.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 21.7)
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 20.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 155.2)
  and 4 more
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 38.2)
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 32.7)
  no clan defined DUF5357; Family of unknown function (DUF5357) (PF17310; HMM-score: 7.8)
  MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008854
  • TAT(Tat/SPI): 0.000287
  • LIPO(Sec/SPII): 0.005548
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 446106564 NCBI
• RefSeq: WP_000184419 NCBI
• UniProt: see NWMN_2166

⊟Protein sequence

• MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQAFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDGSLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLGMLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIAKEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRLMDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKVSPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLIHGRHVTRNAMN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications