PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS12850 [old locus tag: NWMN_2229 ]
• pan locus tag^?: SAUPAN005813000
• symbol: NWMN_RS12850
• pan gene symbol^?: —
• synonym:
• product: oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS12850 [old locus tag: NWMN_2229 ]
• symbol: NWMN_RS12850
• product: oxidoreductase
• replicon: chromosome
• strand: -
• coordinates: 2450492..2451373
• length: 882
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2450492..2451373) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2229

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCAGCTCAAGATCCTAGAACAAAATTTAAAACAACGGATTATGAAAAACAAGAACAA
  GAGGTACCGGGTTTACAATCTGAAATGACGCCAGCACCCGATTGTGGCGAAACGTCTTAC
  CAAGGTCATCAACGCTTACAAGGCTATAAAATATTAGTCACAGGTGGTGACTCAGCAATA
  GGGCGTGCAGCAGCAATTGCGTATGCTAAAGAAGGTGCAGATGTAGCGATTAACTATCTT
  CCGAGTGAAGAACAAGATGCACAAGAAGTACGCCAAGTGATTGAAGAAAGTGGTCAAAAG
  GCAGTGTTAATTCCTGGTGATATAAGAGATGAACAGTTCAACTATGACCTTGTTGAACAA
  GCATATCAACAATTAGGTGGCTTAGATAATGTTACTTTAGTTGCTGGACATCAACAGTAT
  CATGATGATATTCATGGATTTACAACTGAGGCATTTACAGAAACATTTGAAACGAATGTC
  TATCCGCTATTTTGGACAGTTCAAAAAGCATTGGAATATTTAAAACCGGGTGCGTCAATT
  ACAACGACATCATCTGTACAAGGTTATAATCCTAGTCCTATTCTTCATGATTATGCCGCT
  TCAAAAGCAGCAATTATTTCATTAACTAAAAGTTTTTCAGAAGAATTAGGACCAAAAGGA
  ATTAGAGTGAACTGTGTAGCGCCTGGTCCATTCTGGTCACCATTACAAATTTCTGGTGGA
  CAACCTCAAAGTAAAATCCCAACATTTGGTCAAAAAACACCTTTAGGTCGTGCAGGTCAA
  CCTGTTGAGTTATGTGGAACTTATGTGCTATTAGCGTCAGAAGAATCAAGTTATACAACC
  GGACAAGTGTTCGGTGTTAGCGGTGGAGTGCAAATAGATTAG
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⊟Protein

⊟General

• locus tag: NWMN_RS12850 [old locus tag: NWMN_2229 ]
• symbol: NWMN_RS12850
• description: oxidoreductase
• length: 293
• theoretical pI: 4.3889
• theoretical MW: 31734
• GRAVY: -0.395563

⊟Function

• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 139.7)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 127.1)
  Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 117.9)
  and 10 more
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 104.6)
  2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 94.5)
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 92.4)
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 90.5)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 90.1)
  cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 82.5)
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 77.8)
  rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 72.6)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 62.5)
  pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 56.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 177.7)
  and 4 more
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 142)
  KR; KR domain (PF08659; HMM-score: 33.2)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 19.6)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.072169
  • TAT(Tat/SPI): 0.003634
  • LIPO(Sec/SPII): 0.002331
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446652697 NCBI
• RefSeq: WP_000730043 NCBI
• UniProt: see NWMN_2229

⊟Protein sequence

• MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQRLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAVLIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNVYPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGPKGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEESSYTTGQVFGVSGGVQID

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB see NWMN_2229

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications