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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS14060 [old locus tag: NWMN_2446 ]
- pan locus tag?: SAUPAN006207000
- symbol: NWMN_RS14060
- pan gene symbol?: mvaS
- synonym:
- product: hydroxymethylglutaryl-CoA synthase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS14060 [old locus tag: NWMN_2446 ]
- symbol: NWMN_RS14060
- product: hydroxymethylglutaryl-CoA synthase
- replicon: chromosome
- strand: +
- coordinates: 2688971..2690137
- length: 1167
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1141ATGACAATAGGTATCGATAAAATAAACTTTTACGTTCCAAAGTACTATGTAGACATGGCT
AAATTAGCAGAAGCACGCCAAGTAGACCCAAACAAATTTTTAATTGGAATTGGTCAAACT
GAAATGGCTGTTAGTCCTGTAAACCAAGACATCGTTTCAATGGGCGCTAACGCTGCTAAG
GACATTATAACAGACGAAGACAAAAAGAAAATTGGTATGGTAATTGTGGCAACTGAATCA
GCAGTTGATGCTGCTAAAGCAGCCGCTGTTCAAATTCACAACTTATTAGGTATTCAACCT
TTTGCACGCTGCTTTGAAATGAAAGAAGCTTGTTATGCTGCAACACCAGCAATTCAATTA
GCTAAAGATTATTTAGCAACTAGACCGAATGAAAAAGTATTAGTTATTGCTACAGATACA
GCACGTTATGGATTGAATTCAGGCGGCGAGCCAACACAAGGTGCTGGCGCAGTTGCGATG
GTTATTGCACATAATCCAAGCATTTTGGCATTAAATGAAGATGCTGTTGCTTACACTGAA
GACGTTTATGATTTCTGGCGTCCAACTGGACATAAATATCCATTAGTTGATGGTGCATTA
TCTAAAGATGCTTATATCCGCTCATTCCAACAAAGCTGGAATGAATACGCAAAACGTCAA
GGTAAGTCGCTAGCTGACTTCGCATCTCTATGCTTCCATGTTCCATTTACAAAAATGGGT
AAAAAGGCATTAGAGTCAATCATTGATAACGCTGATGAAACAACTCAAGAGCGTTTACGT
TCAGGATATGAAGATGCTGTAGATTATAACCGTTATGTCGGTAATATTTATACTGGATCA
TTATATTTAAGCCTAATATCATTACTTGAAAATCGAGATTTACAAGCTGGTGAAACAATC
GGTTTATTCAGTTATGGCTCAGGTTCAGTTGGTGAATTTTATAGTGCGACATTAGTTGAA
GGCTACAAAGATCATTTAGATCAAGCTGCACATAAAGCATTATTAAATAACCGTACTGAA
GTATCTGTTGATGCATATGAAACATTCTTCAAACGTTTTGATGACGTTGAATTTGACGAA
GAACAAGATGCTGTTCATGAAGATCGTCATATTTTCTACTTATCAAATATTGAAAATAAC
GTTCGTGAATATCACAGACCAGAGTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS14060 [old locus tag: NWMN_2446 ]
- symbol: NWMN_RS14060
- description: hydroxymethylglutaryl-CoA synthase
- length: 388
- theoretical pI: 4.67485
- theoretical MW: 43205.2
- GRAVY: -0.295361
⊟Function[edit | edit source]
- TIGRFAM: hydroxymethylglutaryl-CoA synthase (TIGR01835; EC 2.3.3.10; HMM-score: 531.4)and 3 morehydroxymethylglutaryl-CoA synthase (TIGR01833; EC 2.3.3.10; HMM-score: 160.5)Fatty acid and phospholipid metabolism Biosynthesis putative hydroxymethylglutaryl-CoA synthase (TIGR00748; EC 2.3.3.10; HMM-score: 98.4)Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 43.1)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: Thiolase (CL0046) HMG_CoA_synt_C; Hydroxymethylglutaryl-coenzyme A synthase C terminal (PF08540; HMM-score: 96.8)and 4 moreHMG_CoA_synt_N; Hydroxymethylglutaryl-coenzyme A synthase N terminal (PF01154; HMM-score: 69.1)Chal_sti_synt_N; Chalcone and stilbene synthases, N-terminal domain (PF00195; HMM-score: 27)ACP_syn_III; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III (PF08545; HMM-score: 26.1)ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 17.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.006327
- TAT(Tat/SPI): 0.000235
- LIPO(Sec/SPII): 0.000473
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAVSPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFARCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAMVIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAKRQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNIYTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKALLNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
NWMN_RS02645 ribose-phosphate pyrophosphokinase [1] (data from MRSA252) NWMN_RS02965 elongation factor Tu [1] (data from MRSA252) NWMN_RS03640 LysR family transcriptional regulator [1] (data from MRSA252) NWMN_RS04590 NADH dehydrogenase [1] (data from MRSA252) NWMN_RS06575 30S ribosomal protein S2 [1] (data from MRSA252) NWMN_RS07605 serine/threonine dehydratase [1] (data from MRSA252) NWMN_RS08865 aldehyde dehydrogenase [1] (data from MRSA252) NWMN_RS08905 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) NWMN_RS08930 pyruvate kinase [1] (data from MRSA252) NWMN_RS08995 universal stress protein UspA [1] (data from MRSA252) NWMN_RS09310 proline dehydrogenase [1] (data from MRSA252) NWMN_RS12340 30S ribosomal protein S5 [1] (data from MRSA252) NWMN_RS12395 30S ribosomal protein S3 [1] (data from MRSA252) NWMN_RS14010 pyruvate oxidase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CcpA see NWMN_2446
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)