PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS14150 [old locus tag: NWMN_2463 ]
• pan locus tag^?: SAUPAN006227000
• symbol: NWMN_RS14150
• pan gene symbol^?: crtQ
• synonym:
• product: 4,4'-diaponeurosporenoate glycosyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS14150 [old locus tag: NWMN_2463 ]
• symbol: NWMN_RS14150
• product: 4,4'-diaponeurosporenoate glycosyltransferase
• replicon: chromosome
• strand: -
• coordinates: 2710670..2711797
• length: 1128
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2710670..2711797) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2463

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
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  ATGAAATGGTTATCACGAATATTAACAGTAATAGTGACCATGTCTATGGCGTGTGGTGCA
  TTGATATTTAATCGTAGACATCAGCTAAAGGCGAAAACGCTGAACTTCAATCATAAAGCA
  TTAACAATTATTATTCCGGCTAGAAACGAAGAAAAAAGAATAGGTCATTTACTACATTCG
  ATAATACAACAGCAAGTTCCAGTAGATGTCATTGTTATGAATGACGGATCGACAGATGAA
  ACAGCACGTGTAGCACGTTCATATGGTGCGACTGTAGTAGATGTTGTTGATGATACTGAC
  GGCAAATGGTATGGGAAATCACATGCTTGTTATCAAGGTGTGACGCATGCATGTACGAAT
  CGCATTGCCTTTGTAGATGCTGATGTAACTTTCTTAAGGAAAGATGCTGTTGAAACGTTG
  ATTAATCAGTATCAATTACAAGGTGAAAAAGGATTGTTAAGCGTACAGCCTTATCATATA
  ACAAAGCGTTTCTACGAAGGGTTTTCAGCGATATTTAATTTAATGACAGTCGTTGGTATG
  AATGTATTTTCTACCTTAGACGACGGTCGGACTAACCAGCATGCATTTGGACCGGTGACA
  TTAACAAATAAAGAAGATTATTATGCAACTGGAGGTCATAAAAGTGCAAACCGTCATATT
  ATTGAAGGATTTGCTTTAGGAAGTGCATATACTTCACAATCATTGCCCGTAACAGTTTAT
  GAAGGGTTTCCATTTGTTGCATTTCGCATGTATCAAGAAGGATTTCAGTCATTACAAGAA
  GGATGGACAAAGCATTTGTCAACTGGGGCAGGTGGCACAAAGCCTAAGATCATGACAGCA
  ATTGTGTTGTGGTTGTTTGGTTCTATAGCGAGTATTTTAGGGCTATGTCTTAGTTTAAAA
  TATCGCCAAATGTCTGTAAGAAAAATGGTAGCACTTTACTTGAGCTATACTACACAATTT
  ATTTATCTGCATCGAAGGGTCGGCCAATTTTCTAATTTATTAATGGTATGTCATCCATTG
  TTATTTATGTTTTTTACTAAAATTTTCATCCAATCTTGGAAACAAACGCATCGTTATGGT
  GTAGTTGAATGGAAAGGTCGTCAATATTCTATATCTAAAGAACAATAA
  60
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  1128
  

⊟Protein

⊟General

• locus tag: NWMN_RS14150 [old locus tag: NWMN_2463 ]
• symbol: NWMN_RS14150
• description: 4,4'-diaponeurosporenoate glycosyltransferase
• length: 375
• theoretical pI: 9.84953
• theoretical MW: 42543.9
• GRAVY: -0.044

⊟Function

• reaction:
  EC 2.4.1.-?  ExPASy
• TIGRFAM:
  hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 60.4)
  and 8 more
  Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 47.4)
  poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 45.9)
  glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 42.4)
  mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 42.2)
  putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 29.9)
  hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 15.1)
  Hypothetical proteins Conserved TIGR00454 family protein (TIGR00454; HMM-score: 13.1)
  glycosyltransferase domain (TIGR04440; EC 2.-.-.-; HMM-score: 12.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 61.2)
  and 6 more
  Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 44.5)
  Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 37.3)
  NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 20.2)
  Glyco_transf_21; Glycosyl transferase family 21 (PF13506; HMM-score: 17.7)
  Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 16.9)
  2heme_cytochrom (CL0328) Ferric_reduct; Ferric reductase like transmembrane component (PF01794; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.019225
  • TAT(Tat/SPI): 0.003522
  • LIPO(Sec/SPII): 0.936878
  • Cleavage Site: CS pos: 17-18. SMA-CG. Pr: 0.9397
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 446794475 NCBI
• RefSeq: WP_000871731 NCBI
• UniProt: see NWMN_2463

⊟Protein sequence

• MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTIIIPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGKWYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHITKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANRHIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPKIMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLLMVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB see NWMN_2463

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications