PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0044 [new locus tag: SA_RS00365 ]
• pan locus tag^?: SAUPAN000110000
• symbol: SA0044
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0044 [new locus tag: SA_RS00365 ]
• symbol: SA0044
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 52363..53427
• length: 1065
• essential: no DEG

⊟Accession numbers

• Gene ID: 1122818 NCBI
• RefSeq: NP_373284 NCBI
• BioCyc: see SA_RS00365
• MicrobesOnline: 102310 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGGAACAATACAAAGAAGTACACATTAATTCATTAAACAAAGAAGAATTAGAAAAATTG
  GCAAAAATGGGTCAATTGATTGATGTTAGAACAAAAGAAGAATATGAATTAGGACACATT
  AACGGTTCAACTTTACACCCAGTAGAAGAGATTAAATCATTCAATAAGAATAGCAACAAA
  ACGTATTATATACATTGTAAAAGCGGTAACCGAAGCACTAAAGCTTGCGATTATTTAGCC
  AAACAAGGTTATGATGTCGTGAATTTAAAGGGTGGATATAAAGATTATGAAGCAAAGAAC
  TTCAATAGTGCTCCTTTAATAGAAAAAGATATTGAAATCAAAGAGAATCGTAAACAATTT
  GATTTTAGAGGTCTTCAGTGCCCTGGACCTATTGTTAATATCAGTAAAGAAATTAACAAT
  ATTTCAACGGGTGAGCAAATCGAGGTAACAGTTACAGATCCTGGTTTCAATAGTGATATT
  AAAAGTTGGGCGAAACAAACAGGGAATACCCTTGTAAATCTTACTGAAGAAGCAAATGTA
  ATTAATGCAATTATTCAAAAAGAAAAGCCAAAAGAATTTGAAATCAATGACACTGCTACA
  GGTACAACAATTGTTTTATTTAGCGGTGAGTTAGATAAGGCAGTAGCAGCAATGATCATC
  GCAAATGGCGCTAAAGCAGCAGGTAAAGATGTAACTATTTTCTTTACTTTTTGGGGCCTC
  AACGCATTAAAAAAAGCGCAATCAACTCGCGTTAAAAAGAAAGGTATTTCCAAAATGTTT
  GACCTTATGTTACCTAAGGATCCAATACATATGCCATTATCAAAAATGAATATGTTTGGA
  CTAGGTAATATTATGATGCGTTATGTAATGAACAAGAAAAATGTTGATTCTTTATACTCA
  CTTATCGATCAAGCAATCGATCAAGATATAAAGTTAATTGCTTGTACCATGAGTATGGAT
  GTTATGGGAATTTCAAAAGAAGAATTAAGAGATGAAGTCGATTATGGTGGCGTAGGTACT
  TATATTGGACATACTGAGCAAGCAAATCATAACTTATTTATCTAA
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⊟Protein

⊟General

• locus tag: SA0044 [new locus tag: SA_RS00365 ]
• symbol: SA0044
• description: hypothetical protein
• length: 354
• theoretical pI: 6.88036
• theoretical MW: 39758.3
• GRAVY: -0.418079

⊟Function

• TIGRFAM:
  phage shock operon rhodanese PspE (TIGR02981; EC 2.8.1.1; HMM-score: 32.8)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 30.4)
  selenium metabolism protein YedF (TIGR03527; HMM-score: 28.8)
  and 1 more
  thiazole biosynthesis domain (TIGR04271; HMM-score: 15.9)
• TheSEED  :
  • Disulfide bond regulator
• PFAM:
  DsrEFH-like (CL0394) DrsE_2; DsrE/DsrF/DrsH-like family (PF13686; HMM-score: 187.4)
  and 6 more
  TusA-like (CL0397) TusA; Sulfurtransferase TusA (PF01206; HMM-score: 65.2)
  Phosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 48.4)
  DsrEFH-like (CL0394) DrsE; DsrE/DsrF-like family (PF02635; HMM-score: 39.9)
  no clan defined UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 14.6)
  6_Hairpin (CL0059) Prenyltrans; Prenyltransferase and squalene oxidase repeat (PF00432; HMM-score: 12.8)
  OB (CL0021) RNA_pol_RpbG; DNA-directed RNA polymerase, subunit G (PF16992; HMM-score: 8.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.020736
  • TAT(Tat/SPI): 0.001823
  • LIPO(Sec/SPII): 0.00172
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925751 NCBI
• RefSeq: NP_373284 NCBI
• UniProt: A0A0H3JN38 UniProt

⊟Protein sequence

• MEQYKEVHINSLNKEELEKLAKMGQLIDVRTKEEYELGHINGSTLHPVEEIKSFNKNSNKTYYIHCKSGNRSTKACDYLAKQGYDVVNLKGGYKDYEAKNFNSAPLIEKDIEIKENRKQFDFRGLQCPGPIVNISKEINNISTGEQIEVTVTDPGFNSDIKSWAKQTGNTLVNLTEEANVINAIIQKEKPKEFEINDTATGTTIVLFSGELDKAVAAMIIANGAKAAGKDVTIFFTFWGLNALKKAQSTRVKKKGISKMFDLMLPKDPIHMPLSKMNMFGLGNIMMRYVMNKKNVDSLYSLIDQAIDQDIKLIACTMSMDVMGISKEELRDEVDYGGVGTYIGHTEQANHNLFI

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0042 < SA0043 < SA0044

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications