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Pangenome04-0298108BA0217611819-97685071193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0047 [new locus tag: SACOL_RS00250 ]
  • pan locus tag?: SAUPAN000110000
  • symbol: SACOL0047
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0047 [new locus tag: SACOL_RS00250 ]
  • symbol: SACOL0047
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 54775..55842
  • length: 1068
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCACAATATAAAACGAAACACATTAATGATTTCACTAAAAAAGAATTACAAGAATTG
    AGTTCTAAGGGTCAATTAATTGACGTTCGACATCCTGAAGAATTTGAATTAGGACACATT
    AAAAACGCTTTATTACATTCAGTTGAAAATATAGAGACTTTTAAACAAAGCAAAAATAAA
    ACTTACTATATCTATTGTAAAAGTGGTAATAGAAGTAGAAAAGCCAGTCAATTTCTTGCA
    CAACGCGGATACGATGTAGTGAATTTAGACGGAGGTTATACCGCATACGAACAACAACAT
    CATAATGATAATTTATCTAAAGTAAAAGAAGATAGAGAAATTAAAGATAATCGTAAGACA
    TTCAATTATAGTAATCTACAGTGTCCAGGACCTATTGTTAATATTAGTAAAGAAATCAAA
    AACATCGCTATTGGCGATCAAATAGAAGTTGTTGTGACTGATCACGGATTTTTAAATGAT
    ATTAAAAGTTGGGTCAAACAAACTGGACATACGCTTGTTAGACTCAACGATTTTGGCAAT
    GAAATTCGCGCTATTATTCAAAAAGAAGAAAATAAAAATATAGAAGTGACACACACGAAA
    AGTGGAACAACAATTGTTTTATTTAGCGGCGAGCTAGATAAAGCAGTTGCAGCAATGATT
    ATTGCTAATGGTGCTAAAGCTGCAGGTAGAGATGTAACCATTTTTTGCACATTTTGGGGC
    TTAAATGCACTTAAAAAAATTCAAAGTCAACGTATTAAGAAAAAAGGTATTGCAAAACTT
    TTTGACTTCATGCTTCCAAATTCACCAATTAACATGCCTATTTCTAAAATGAATATGTTT
    GGTTTAGGCAATCTAATGATGCGTTATGTCATGAAAAAGAAAAATGTCGATACTTTACCT
    TCTCTTATTGATCAAGCAGTTGAGCAAGGTGTTAAACTTATCGCTTGTACAATGAGTATG
    GATGTGATGGGCATTACAAAAGAAGAACTACGTGATGATGTAGAATACGGTGGCGTAGGT
    GCATATATTGGATATACAGAACAAGCTAATCACAATTTATTTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1068

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0047 [new locus tag: SACOL_RS00250 ]
  • symbol: SACOL0047
  • description: hypothetical protein
  • length: 355
  • theoretical pI: 9.29673
  • theoretical MW: 40204.9
  • GRAVY: -0.434085

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 37.6)
    phage shock operon rhodanese PspE (TIGR02981; EC 2.8.1.1; HMM-score: 34.2)
    and 2 more
    selenium metabolism protein YedF (TIGR03527; HMM-score: 27.2)
    thiazole biosynthesis domain (TIGR04271; HMM-score: 22.6)
  • TheSEED:  
    Disulfide bond regulator
  • PFAM:
    DsrEFH-like (CL0394) DrsE_2; DsrE/DsrF/DrsH-like family (PF13686; HMM-score: 184.7)
    and 5 more
    TusA-like (CL0397) TusA; Sulfurtransferase TusA (PF01206; HMM-score: 62.8)
    Phosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 57.8)
    DsrEFH-like (CL0394) DrsE; DsrE/DsrF-like family (PF02635; HMM-score: 40)
    no clan defined UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 24.4)
    DUF413; Protein of unknown function, DUF (PF04219; HMM-score: 13.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009068
    • TAT(Tat/SPI): 0.000415
    • LIPO(Sec/SPII): 0.000824
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSQYKTKHINDFTKKELQELSSKGQLIDVRHPEEFELGHIKNALLHSVENIETFKQSKNKTYYIYCKSGNRSRKASQFLAQRGYDVVNLDGGYTAYEQQHHNDNLSKVKEDREIKDNRKTFNYSNLQCPGPIVNISKEIKNIAIGDQIEVVVTDHGFLNDIKSWVKQTGHTLVRLNDFGNEIRAIIQKEENKNIEVTHTKSGTTIVLFSGELDKAVAAMIIANGAKAAGRDVTIFCTFWGLNALKKIQSQRIKKKGIAKLFDFMLPNSPINMPISKMNMFGLGNLMMRYVMKKKNVDTLPSLIDQAVEQGVKLIACTMSMDVMGITKEELRDDVEYGGVGAYIGYTEQANHNLFI

Experimental data[edit | edit source]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]