PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0078 [new locus tag: SA_RS00540 ]
• pan locus tag^?: SAUPAN000832000
• symbol: SA0078
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0078 [new locus tag: SA_RS00540 ]
• symbol: SA0078
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 87692..89425
• length: 1734
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1122852 NCBI
• RefSeq: NP_373319 NCBI
• BioCyc: see SA_RS00540
• MicrobesOnline: 102345 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATTTATAAATCTATTTATAATTTTCAGGAAACAAATACAAATTTTTTAGAGAATCTAGAA
  TCTTTAAATGATGACAATTATGAACTGCTTAATGATAAAGAACTTGTTAGTGATTCAAAT
  GAATTAAAATTAATTAGTAAAGTTTATATACGTAAAAAAGACAAAAAACTATTAGATTGG
  CAATTATTAATAAAGAATGTATACCTAGATACTGAAGAAGATGACAATTTATTTTCAGAA
  TCCGGTCATCATTTTGATGCAATATTATTTCTCAAAGAAGATACTACATTACAAAATAAT
  GTATATATTATACCTTTTGGACAAGCATATCATGATATAAATAATTTGATTGATTATGAC
  TTCGGAATTGATTTTGCAGAAAGAGCAATCAAAAATGAAGACATAGTTAATAAAAATGTT
  AATTTTTTTCAACAAAACAGGCTTAAAGAGATTGTTAATTATAGAAGGAATAGTGTAGAT
  TACGTTAGACCTTCAGAATCTTATATATCAGTCCAAGGACATCCACAGAATCCTCAAATT
  TTTGGAAAAACGATGACTTGTGGTACAAGTATTTCATTGCGTGTACCGAATAGAAAGCAG
  CAATTCATAGATAAAATTAGTGTGATAATCAAAGAAATAAACGCTATTATTAATCTTCCT
  CAAAAAATTAGTGAATTTCCTAGAATAGTAACTTTAAAAGACTTGAATAAAATAGAAGTA
  TTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAAAATATATCTATA
  GATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGATGATATGCTCGAC
  GTCAATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTTGATGCGACTGAT
  CCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATATGACGTTAATTCT
  ATAAATGATGTCAGAATTGAAGTGATAGATAATTTGGGGCATTCAAATAATATGCTACTA
  AAAACGATACTACATGCAGAAGTTGAAATGGAAGATGGAAAGAAATATTTATTACAAAAT
  GGGAAATGGGGTTATTTTAATAGAGAATTTTTTGACCTTTTGAATGATCATTTGAATGAG
  ATAGAAATTAGGTATAACACACTAACTCCCACAGGTTTAGTATTTAAAGAAGGAGAAGAA
  GGATATATAAAAGAAATAGTAGGAAGATTGCCAGAAGAATATTTAATGTTACATAAAAAA
  TTCATAAAACCAATAAATAAGAATTTTATAGTAAAAGGAAATGGAATAGAGTTGGCAGAC
  TTATACAATATTAAAAACAAAGAGCTTTTCACGATTAAAAGAGGAATTAATACATCTTTA
  TCTCTTTATAGTCTAGAACAGAATATAATAGCAATTAACGCCTTAAAATATCCAGAATCA
  TATAATTTTGAAGAATTAATAGAAGCTATTCCTGATAACTCGGAAAATATATTTAATGAT
  ATACAACGGAGTACAAATTTTAGTATAGTATGGATTTTACCGATATCATCTATTGATAAT
  ATGCCCATAAAAGATATGGTTCATACTAGTAACGTAATAAATAAAAACTTTCAATTAACT
  AATTTAGGTTCAGTATTACTTAAAAATAAATTAGTAGAGTGGTCTCTCTATTTAAAAGAT
  CAAAGAATTAATCCAATTATTTATATGGAAACGCCAACTGAAGATAGAAATTAA
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  1734
  

⊟Protein

⊟General

• locus tag: SA0078 [new locus tag: SA_RS00540 ]
• symbol: SA0078
• description: hypothetical protein
• length: 577
• theoretical pI: 4.65848
• theoretical MW: 67312.1
• GRAVY: -0.359965

⊟Function

• TIGRFAM:
  sporadically distributed protein, TIGR04141 family (TIGR04141; HMM-score: 90.6)
• TheSEED  :
  • hypothetical protein
• PFAM:
  Concanavalin (CL0004) Exotox-A_bind; Exotoxin A binding (PF09101; HMM-score: 9.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002539
  • TAT(Tat/SPI): 0.00016
  • LIPO(Sec/SPII): 0.000475
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925786 NCBI
• RefSeq: NP_373319 NCBI
• UniProt: A0A0H3JKT9 UniProt

⊟Protein sequence

• MYKSIYNFQETNTNFLENLESLNDDNYELLNDKELVSDSNELKLISKVYIRKKDKKLLDWQLLIKNVYLDTEEDDNLFSESGHHFDAILFLKEDTTLQNNVYIIPFGQAYHDINNLIDYDFGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNYRRNSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKEINAIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKLSNSSTTENISIDISRFLELSNMILLLDDMLDVNIYINSFKNNTLETFDATDPEVDYITEIGDYLLKYDVNSINDVRIEVIDNLGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGYFNREFFDLLNDHLNEIEIRYNTLTPTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIKPINKNFIVKGNGIELADLYNIKNKELFTIKRGINTSLSLYSLEQNIIAINALKYPESYNFEELIEAIPDNSENIFNDIQRSTNFSIVWILPISSIDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVEWSLYLKDQRINPIIYMETPTEDRN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications