PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00540 [old locus tag: SA0078 ]
• pan locus tag^?: SAUPAN000832000
• symbol: SA_RS00540
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00540 [old locus tag: SA0078 ]
• symbol: SA_RS00540
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 87680..89425
• length: 1746
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (87680..89425) NCBI
• BioCyc: SA_RS00540 BioCyc
• MicrobesOnline: see SA0078

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  301
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  421
  481
  541
  601
  661
  721
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  841
  901
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  1021
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  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  
  ATGAAGATTAATATTTATAAATCTATTTATAATTTTCAGGAAACAAATACAAATTTTTTA
  GAGAATCTAGAATCTTTAAATGATGACAATTATGAACTGCTTAATGATAAAGAACTTGTT
  AGTGATTCAAATGAATTAAAATTAATTAGTAAAGTTTATATACGTAAAAAAGACAAAAAA
  CTATTAGATTGGCAATTATTAATAAAGAATGTATACCTAGATACTGAAGAAGATGACAAT
  TTATTTTCAGAATCCGGTCATCATTTTGATGCAATATTATTTCTCAAAGAAGATACTACA
  TTACAAAATAATGTATATATTATACCTTTTGGACAAGCATATCATGATATAAATAATTTG
  ATTGATTATGACTTCGGAATTGATTTTGCAGAAAGAGCAATCAAAAATGAAGACATAGTT
  AATAAAAATGTTAATTTTTTTCAACAAAACAGGCTTAAAGAGATTGTTAATTATAGAAGG
  AATAGTGTAGATTACGTTAGACCTTCAGAATCTTATATATCAGTCCAAGGACATCCACAG
  AATCCTCAAATTTTTGGAAAAACGATGACTTGTGGTACAAGTATTTCATTGCGTGTACCG
  AATAGAAAGCAGCAATTCATAGATAAAATTAGTGTGATAATCAAAGAAATAAACGCTATT
  ATTAATCTTCCTCAAAAAATTAGTGAATTTCCTAGAATAGTAACTTTAAAAGACTTGAAT
  AAAATAGAAGTATTAGATACTTTATTGCTAAAAAAACTATCGAATTCTTCAACTACAGAA
  AATATATCTATAGATATATCAAGATTTTTAGAACTAAGTAATATGATACTCTTGTTAGAT
  GATATGCTCGACGTCAATATATATATAAATTCTTTTAAAAATAATACATTAGAGACATTT
  GATGCGACTGATCCTGAAGTTGATTACATCACTGAAATAGGAGATTACTTATTAAAATAT
  GACGTTAATTCTATAAATGATGTCAGAATTGAAGTGATAGATAATTTGGGGCATTCAAAT
  AATATGCTACTAAAAACGATACTACATGCAGAAGTTGAAATGGAAGATGGAAAGAAATAT
  TTATTACAAAATGGGAAATGGGGTTATTTTAATAGAGAATTTTTTGACCTTTTGAATGAT
  CATTTGAATGAGATAGAAATTAGGTATAACACACTAACTCCCACAGGTTTAGTATTTAAA
  GAAGGAGAAGAAGGATATATAAAAGAAATAGTAGGAAGATTGCCAGAAGAATATTTAATG
  TTACATAAAAAATTCATAAAACCAATAAATAAGAATTTTATAGTAAAAGGAAATGGAATA
  GAGTTGGCAGACTTATACAATATTAAAAACAAAGAGCTTTTCACGATTAAAAGAGGAATT
  AATACATCTTTATCTCTTTATAGTCTAGAACAGAATATAATAGCAATTAACGCCTTAAAA
  TATCCAGAATCATATAATTTTGAAGAATTAATAGAAGCTATTCCTGATAACTCGGAAAAT
  ATATTTAATGATATACAACGGAGTACAAATTTTAGTATAGTATGGATTTTACCGATATCA
  TCTATTGATAATATGCCCATAAAAGATATGGTTCATACTAGTAACGTAATAAATAAAAAC
  TTTCAATTAACTAATTTAGGTTCAGTATTACTTAAAAATAAATTAGTAGAGTGGTCTCTC
  TATTTAAAAGATCAAAGAATTAATCCAATTATTTATATGGAAACGCCAACTGAAGATAGA
  AATTAA
  60
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  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1746
  

⊟Protein

⊟General

• locus tag: SA_RS00540 [old locus tag: SA0078 ]
• symbol: SA_RS00540
• description: hypothetical protein
• length: 581
• theoretical pI: 4.6922
• theoretical MW: 67780.7
• GRAVY: -0.354733

⊟Function

• TIGRFAM:
  sporadically distributed protein, TIGR04141 family (TIGR04141; HMM-score: 90.6)
• TheSEED: see SA0078
• PFAM:
  Concanavalin (CL0004) Exotox-A_bind; Exotoxin A binding (PF09101; HMM-score: 9.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003722
  • TAT(Tat/SPI): 0.000085
  • LIPO(Sec/SPII): 0.000372
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446622844 NCBI
• RefSeq: WP_000700190 NCBI
• UniProt: see SA0078

⊟Protein sequence

• MKINIYKSIYNFQETNTNFLENLESLNDDNYELLNDKELVSDSNELKLISKVYIRKKDKKLLDWQLLIKNVYLDTEEDDNLFSESGHHFDAILFLKEDTTLQNNVYIIPFGQAYHDINNLIDYDFGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNYRRNSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKEINAIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKLSNSSTTENISIDISRFLELSNMILLLDDMLDVNIYINSFKNNTLETFDATDPEVDYITEIGDYLLKYDVNSINDVRIEVIDNLGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGYFNREFFDLLNDHLNEIEIRYNTLTPTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIKPINKNFIVKGNGIELADLYNIKNKELFTIKRGINTSLSLYSLEQNIIAINALKYPESYNFEELIEAIPDNSENIFNDIQRSTNFSIVWILPISSIDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVEWSLYLKDQRINPIIYMETPTEDRN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications