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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0092 [new locus tag: SA_RS00620 ]
  • pan locus tag?: SAUPAN000860000
  • symbol: SA0092
  • pan gene symbol?: csa1A
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0092 [new locus tag: SA_RS00620 ]
  • symbol: SA0092
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 103853..104623
  • length: 771
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATGAAACGATTAAACAAATTAGTGTTAGGCATTATTTTTCTGTTTTTAGTCATTAGT
    ATCACTGCTGGTTGTGGCATAGGTAAAGAAGCGGAAGTTAAGAAAAGCTTTGAAAAAACA
    TTGAGTATGTACCCTATTAAAAATCTAGAGGATTTATACGATAAGGAAGGCTATCGTGAT
    GATCAGTTTGATAAAAATGATAAAGGTACATGGATTATAAATTCTGAAATGGTTATTCAA
    CCTAATAATGAAGATATGGTAGCTAAAGGCATGGTTCTATATATGAATAGAAATACCAAA
    ACAACAAATGGTTACTACTATGTCGATGTGACTAAGGACGAGGATGAAGGAAAACCGCAC
    GACAATGAAAAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAA
    ATTAAAGATAAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGA
    AACTTTAAAGATTTGTCGAAGTACAAAGATGGAGATATTTCATACAATCCAGAGGTGCCA
    AGTTATTCAGCAAAATATCAAGTAACTAATGATGATTATAATGTAAAACAATTACGTAAA
    AGATATGATATACCGACGAATAAAGCACCAAAGTTATTGTTGAAAGGTACAGGTAATTTA
    AAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACTTTCGTGGAGAAAAAAGGTGAA
    AATATATACTTTAGTGATAGTCTACATCTTGAACCAAGTGAGGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    771

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0092 [new locus tag: SA_RS00620 ]
  • symbol: SA0092
  • description: hypothetical protein
  • length: 256
  • theoretical pI: 8.66851
  • theoretical MW: 29742.7
  • GRAVY: -0.791406

Function[edit | edit source]

  • TIGRFAM:
    Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 383.3)
  • TheSEED  :
    • FIG01107885: hypothetical protein
  • PFAM:
    LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 412.4)
    and 1 more
    no clan defined DUF3519; Protein of unknown function (DUF3519) (PF12033; HMM-score: 11.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: ITAGCGI
  • SignalP: Signal peptide LIPO(Sec/SPII) length 24 aa
    • SP(Sec/SPI): 0.00126
    • TAT(Tat/SPI): 0.000082
    • LIPO(Sec/SPII): 0.998425
    • Cleavage Site: CS pos: 24-25. TAG-CG. Pr: 0.9990
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMYPIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYGNFKDLSKYKDGDISYNPEVPSYSAKYQVTNDDYNVKQLRKRYDIPTNKAPKLLLKGTGNLKGSSVGYKDIEFTFVEKKGENIYFSDSLHLEPSEDK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Christina Schluepen, Enrico Malito, Ambra Marongiu, Markus Schirle, Elisabeth McWhinnie, Paola Lo Surdo, Marco Biancucci, Fabiana Falugi, Vincenzo Nardi-Dei, Sara Marchi, Maria Rita Fontana, Benedetta Lombardi, Maria Grazia De Falco, C Daniela Rinaudo, Glen Spraggon, Mikkel Nissum, Fabio Bagnoli, Guido Grandi, Matthew J Bottomley, Sabrina Liberatori
Mining the bacterial unknown proteome: identification and characterization of a novel family of highly conserved protective antigens in Staphylococcus aureus.
Biochem J: 2013, 455(3);273-84
[PubMed:23895222] [WorldCat.org] [DOI] (I p)