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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0100 [new locus tag: SAUSA300_RS00520 ]
- pan locus tag?: SAUPAN000860000
- symbol: SAUSA300_0100
- pan gene symbol?: csa1A
- synonym:
- product: tandem lipoprotein
csa1A: tandem conserved staphylococcal antigen 1A
binds extracellular DNA as a lipoprotein during biofilm formation
exact number of tandem repeats varies and can expand or contract over the course of an infection
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0100 [new locus tag: SAUSA300_RS00520 ]
- symbol: SAUSA300_0100
- product: tandem lipoprotein
- replicon: chromosome
- strand: +
- coordinates: 110200..110970
- length: 771
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914666 NCBI
- RefSeq: YP_492817 NCBI
- BioCyc: see SAUSA300_RS00520
- MicrobesOnline: 1291615 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGATGAAACGATTAAACAAATTAGTGTTAGGCATTATTTTTCTGTTTTTAGTCATTAGT
ATCACTGCTGGTTGTGGCATAGGTAAAGAAGCGGAAGTTAAGAAAAGCTTTGAAAAAACA
TTGAGTATGTACCCTATTAAAAATCTAGAGGATTTATACGATAAGGAAGGCTATCGTGAT
GATCAGTTTGATAAAAATGATAAAGGTACATGGATTATAAATTCTGAAATGGTTATTCAA
CCTAATAATGAAGATATGGTAGCTAAAGGCATGGTTCTATATATGAATAGAAATACCAAA
ACAACAAATGGTTACTACTATGTCGATGTGACTAAGGACGAGGATGAAGGAAAACCGCAC
GACAATGAAAAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAA
ATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGC
GACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCG
AGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAA
AGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTA
AAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAA
AATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0100 [new locus tag: SAUSA300_RS00520 ]
- symbol: SAUSA300_0100
- description: tandem lipoprotein
- length: 256
- theoretical pI: 8.65284
- theoretical MW: 29786.8
- GRAVY: -0.793359
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 3.33
- Cellwall Score: 3.33
- Extracellular Score: 3.33
- Internal Helix: 1
- LocateP: Lipid anchored
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPII)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: ITAGCGI
- SignalP: Signal peptide LIPO(Sec/SPII) length 24 aa
- SP(Sec/SPI): 0.00126
- TAT(Tat/SPI): 0.000082
- LIPO(Sec/SPII): 0.998425
- Cleavage Site: CS pos: 24-25. TAG-CG. Pr: 0.9990
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMYPIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYGDFKNLKNYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEKKEENIYFSDSLDYKKSGDV
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Christina Schluepen, Enrico Malito, Ambra Marongiu, Markus Schirle, Elisabeth McWhinnie, Paola Lo Surdo, Marco Biancucci, Fabiana Falugi, Vincenzo Nardi-Dei, Sara Marchi, Maria Rita Fontana, Benedetta Lombardi, Maria Grazia De Falco, C Daniela Rinaudo, Glen Spraggon, Mikkel Nissum, Fabio Bagnoli, Guido Grandi, Matthew J Bottomley, Sabrina Liberatori
Mining the bacterial unknown proteome: identification and characterization of a novel family of highly conserved protective antigens in Staphylococcus aureus.
Biochem J: 2013, 455(3);273-84
[PubMed:23895222] [WorldCat.org] [DOI] (I p)
- ↑ Darya Belikova, Angelika Jochim, Jeffrey Power, Matthew T G Holden, Simon Heilbronner
"Gene accordions" cause genotypic and phenotypic heterogeneity in clonal populations of Staphylococcus aureus.
Nat Commun: 2020, 11(1);3526
[PubMed:32665571] [WorldCat.org] [DOI] (I e)Jeffrey S Kavanaugh, Caralyn E Flack, Jessica Lister, Erica B Ricker, Carolyn B Ibberson, Christian Jenul, Derek E Moormeier, Elizabeth A Delmain, Kenneth W Bayles, Alexander R Horswill
Identification of Extracellular DNA-Binding Proteins in the Biofilm Matrix.
mBio: 2019, 10(3);
[PubMed:31239382] [WorldCat.org] [DOI] (I e)