PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0199 [new locus tag: SA_RS01180 ]
• pan locus tag^?: SAUPAN001057000
• symbol: oppB
• pan gene symbol^?: gisB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0199 [new locus tag: SA_RS01180 ]
• symbol: oppB
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 235035..236372
• length: 1338
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1122976 NCBI
• RefSeq: NP_373443 NCBI
• BioCyc: see SA_RS01180
• MicrobesOnline: 102469 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATAATTACTTATTTAGAATCTTTTAAGTCGTTGTATCACAAGGCATTTTACAAGTTTGTA
  TTATCGGTATTAAGCTTGCCAATATTTTTATATGCAGTGATAAAGTTTTTCTTTTCTGCT
  AAGAGAAAAAATTTTTACGCTAATAATTCTGAAATTTCAGAAATTGAACGAGCGTTACAT
  CAAAAATATAAATATTTATCGCAGCAAAAGTCATCCACACAAATACATAAAGAAGCATTA
  AAAATATTCAAGGCACAAAGTTCTAATACGAGTTCAAAGAATATTGAACAAGCACATTTT
  TCAACATACTTTGAAAATTTATTATTTCATAAGTTCATCATGATCAAAGTAATATTGGCC
  TTGCCGATGTTCATCTTATTGACTATTTATTTACAGCCATTAGTTAGATATATTTTTGAG
  CGAATTGTCATGGCTGTGATTGTCATCATTGGTGTTATTGTCAGTGTGTTTACCATTCTG
  TATTTTTCACCGCTTGATGCGGCTTATAGCATACTGGGACAAAATGCAACAAAGGCACAG
  ATACATCAATTCAATGTATTACATCATCTTAACGAACCTTATTTTATTCAATTGTGGGAT
  ACCATCAAGGGTGTTTTTACCTTTGACTTAGGTACGACTTACAAAGGGAATGAGGTTGTG
  ACTAAAGCAGTTGGCGAAAGAATTCCAATTACAATAATTGTCGCAGTATTAGCGCTAATT
  GTGGCATTAATTATTGCAATACCAATTGGTATTATCAGTGCGATGAAGCGAAATAGTTGG
  CTTGATATCACGTTAATGATAATTGCATTAATTGGTTTATCTATTCCAAGTTTCTGGCAA
  GGGCTATTATTCATTTTAGCGTTCTCATTGAAATTGGATATTTTGCCACCATCTTATATG
  CCAGAACATCCAATATCGTTGATTTTACCTGTACTTGTCATTGGAACAAGTATTGCTGCT
  TCTATCACGCGTATGACAAGGTCTTCTGTACTTGAAGTAATGCGCAGCGATTATGTTTTA
  ACTGCTTATGCAAAAGGATTATCGACGACACAAGTTGTTATTAAACATATTTTGAAAAAT
  GCCATTATTCCAATTGTAACGTTAGTTGGTCTTCTAGTGGCAGAGTTACTAGGCGGTTCA
  GCAGTGACGGAACAAGTATTTAACATTAATGGTATCGGTCGTTATATCGTCCAAAAACAA
  CTAATACCTGATATCCCAGCAGTCATGGGTGGGGTCGTATATATATCAATTGTAATATCT
  TTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAA
  ATTAACGAAAGGAAGTGA
  60
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  1320
  1338
  

⊟Protein

⊟General

• locus tag: SA0199 [new locus tag: SA_RS01180 ]
• symbol: OppB
• description: hypothetical protein
• length: 445
• theoretical pI: 9.97083
• theoretical MW: 50050.4
• GRAVY: 0.668539

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 206.3)
  and 1 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 36.1)
• TheSEED  :
  • Putative glutathione transporter, permease component
  Sulfur Metabolism Organic sulfur assimilation Utilization of glutathione as a sulphur source  Putative glutathione transporter, permease component
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 139.3)
  and 2 more
  Transporter (CL0375) Fig1; Ca2+ regulator and membrane fusion protein Fig1 (PF12351; HMM-score: 15.8)
  no clan defined COX6C; Cytochrome c oxidase subunit VIc (PF02937; HMM-score: 7.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 8
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017155
  • TAT(Tat/SPI): 0.000935
  • LIPO(Sec/SPII): 0.003891
• predicted transmembrane helices (TMHMM): 8

⊟Accession numbers

• GI: 15925910 NCBI
• RefSeq: NP_373443 NCBI
• UniProt:

⊟Protein sequence

• MITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEIERALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENLLFHKFIMIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSILGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIPITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILAFSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAKGLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIPDIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: oppB > SA0200 > rlp > SA0202

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications