PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0185 [new locus tag: SACOL_RS00945 ]
• pan locus tag^?: SAUPAN001057000
• symbol: SACOL0185
• pan gene symbol^?: gisB
• synonym:
• product: peptide ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0185 [new locus tag: SACOL_RS00945 ]
• symbol: SACOL0185
• product: peptide ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 216035..217342
• length: 1308
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3236717 NCBI
• RefSeq: YP_185084 NCBI
• BioCyc: see SACOL_RS00945
• MicrobesOnline: 911662 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  TTGTATCACAAGGCATTTTACAAGTTTGTATTATCGGTATTAAGCTTGCCAATATTTTTA
  TATGCAGTGATAAAGTTTTTCTTTTCTGCTAAGAGGAAAAATTTTTACGCTAATAATTCT
  GAAATTTCAGAAATTGAACAGGCGTTACATCAAAAATATAAATATTTATCGCAGCAAAAG
  TCATCCACACAAATACATAAAGAAGCATTAAAAATATTCAAGGCACAAAGTTCTAATACG
  AGTTCAAAGAATATTGAACAAGCACATTTTTCAACATACTTTGAAAATGTATTATTTCAT
  AAGTTCATCATGATCAAAGTGATATTGGCCTTGCCGATGTTCATCTTATTGACTTTTTAT
  TTACAGCCATTAGTTAGATATATTTTTGAACGAATTGTCATGGCTGTGATTGTCATCATT
  GGTGTTATTGTCAGTGTGTTTACCATTCTGTATTTTTCACCGCTTGATGCGGCTTATAGC
  ATACTGGGACAAAATGCAACAAAGGCACAGATACATCAATTCAATGTATTACATCATCTT
  AACGAACCTTATTTTATTCAATTGTGGGATACCATCAAGGGTGTTTTTACCTTTGACTTA
  GGTACGACTTACAAAGGGAATGAGGTTGTGACTAAAGCAGTTGGCGAAAGAATTCCAATT
  ACAATAATTGTCGCAGTATTAGCGCTAATGGTGGCATTAATTATTGCAATACCAATTGGT
  ATTATCAGTGCGATGAAGCGAAATAGTTGGCTTGATATCACGTTAATGATAATTGCATTA
  ATTGGTTTATCTATTCCAAGTTTCTGGCAAGGGCTATTATTCATTTTAGCGTTCTCATTG
  AAATTGGATATTTTGCCACCATCTTATATGCCAGAACATCCAATATCGTTGATTTTACCT
  GTACTTGTCATTGGAACAAGTATTGCTGCTTCTATCACGCGTATGACAAGGTCTTCTGTA
  CTTGAAGTAATGCGCAGCGATTATGTTTTAACTGCTTATGCAAAAGGATTATCGACGACA
  CAAGTTGTTATTAAACATATTTTGAAAAATGCCATTATTCCAATTGTAACGTTAGTTGGT
  CTTCTAGTGGCAGAGTTACTAGGCGGTTCAGCAGTGACGGAACAAGTATTTAACATTAAT
  GGTATCGGGCGTTATATCGTCCAAAAACAACTAATACCTGATATTCCAGCAGTCATGGGT
  GGGGTCGTATATATATCAATTGTAATATCTTTAGCAAACTTAATTATTGATATATTTTAT
  GCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGA
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⊟Protein

⊟General

• locus tag: SACOL0185 [new locus tag: SACOL_RS00945 ]
• symbol: SACOL0185
• description: peptide ABC transporter permease
• length: 435
• theoretical pI: 9.94543
• theoretical MW: 48878
• GRAVY: 0.668276

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 205.8)
  and 1 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 35.8)
• TheSEED  :
  • Putative glutathione transporter, permease component
  Sulfur Metabolism Organic sulfur assimilation Utilization of glutathione as a sulphur source  Putative glutathione transporter, permease component
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 139.4)
  and 3 more
  Transporter (CL0375) Fig1; Ca2+ regulator and membrane fusion protein Fig1 (PF12351; HMM-score: 18.5)
  no clan defined Orbi_VP2; Orbivirus outer capsid protein VP2 (PF00898; HMM-score: 10.4)
  DUF3995; Protein of unknown function (DUF3995) (PF13160; HMM-score: 10.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 8
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.107211
  • TAT(Tat/SPI): 0.002211
  • LIPO(Sec/SPII): 0.035329
• predicted transmembrane helices (TMHMM): 8

⊟Accession numbers

• GI: 57651201 NCBI
• RefSeq: YP_185084 NCBI
• UniProt: A0A0H2WXU5 UniProt

⊟Protein sequence

• MYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEIEQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFIMIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSILGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIPITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILAFSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAKGLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIPDIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SACOL0185 > SACOL0186 > SACOL0187

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications