PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0269 [new locus tag: SA_RS01580 ]
pan locus tag^?: SAUPAN001168000
symbol: SA0269
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0269 [new locus tag: SA_RS01580 ]
• symbol: SA0269
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 324078..325601
• length: 1524
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123048 NCBI
• RefSeq: NP_373515 NCBI
• BioCyc: see SA_RS01580
• MicrobesOnline: 102541 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1501
  
  ATGTCTAAAAAGTTAAAAATTATAATTCCTATTATTATTGTCTTATTATTAATAGGTGGA
  ATCGCATGGGGAGTTTATGCATTTTTTGCAAACACACCGAAAAATACATACTTAAAAAGC
  GAACAACAAACTGCAAAAATGTATAAAGATTATTTTAATGACCGTTTTGAAAATGAAGTG
  AAGTTCCAAGAAAAGATGAAAGATAATTCGTTTTTATCTTCATTAGAATTAAGCGCAGAT
  GCATCAGATGAAATTGTTAAGGGGCTTGGTATTCCTAAATCTGTTGTTAATGCTTCGAAA
  ATTAAAATGTCATATGGACATGATCCTAAAAAAGAGAAATCAATGATTAATCTTGAACCA
  ACAATCGCAGACTCTGCATTAGGGAAATTCCAGTTAGCTGCAGATAAAGATAAGCATTAT
  TTCGAATCACCATTATTTAAAGGGAAATATAGTGTTAATAATTCTGATTTATTATCAACG
  TATTCAAAACTTACAGGTGAAGATGAAGAAACAGCAAAAGAAAATGGTATTACAAACCAA
  CAACTAAATTTAAATACTCTTTTTAGTAATGCTCAAGCACAACAAAGTGACTACAGCAAA
  ATTGCCGAAAAATATTCCGAACTTATTGTCGACAAATTAGATGACGATAATTTTGATAAA
  GGTAAAAAAGAAGAAATTAAGGTTAATGGTGAAAAGTACAAAGTTAGACCTGTCACGTTA
  ACACTTAGCAGAGCTGACACTAAAAAAATTACATTAGCTGTATTAGAAGAAGCTAAAAAG
  GATAAAGACCTTAAAAAATTAATGGAAGAACAAGGTGCTACAAAAGACTATGATAAGGAC
  ATCAAAAAAGCAATTGACGATGTCAAAGAAACTAAAAAGGATGAATTTGCTAAAATTCAA
  TCTAAAATTTATACCGAAAAACATACGATTGTAAAACGAGAAATTACTATTACAGACAAA
  GAAAATAATAAAACTAAAATCAAAGGTACTAATACTTTAGAAGACGATAAGTTAAAACTA
  GATTACGCACTTGATTTCGATCAAGATAAATACACGTATGCTGAAGCGAAATATACAATT
  AAAGGCGTATCTTCTAAGGAAAAAGACAATAAATACAGCGATAAATACGAATTTGGTAAA
  AAGACAGAATATGATGAATCAAAAATCAAATTAGATAACCAAGAAAAAGTAGATGGCACA
  AAACGTCAAGATAAAGGTAAAATCACTGTCGCATTAGATAAATATAGCGACGAAAATGAA
  TTCACTTTTGAAAATAATATAGATTCTGACGTAAAAAATAACACTCAGAAATCTACGTTA
  AATATCGGCATCAAATATGCTGAAGAACCAATTAATTTCATTTTAAAATCTAGCACAAAA
  TTGAAAGCAGATATTGATTTTGATGATAGTGGTGCGAAAGATTTCAATAGTCTATCTTCA
  AAAGACCGTGAAAAACTTGAAAAAGAAATCGAAAAAAATGGCGGCAAAATGTTTGAATCA
  ATTTTAAAAAAGGCATCTAAATAA
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  1524
  

⊟Protein

⊟General

• locus tag: SA0269 [new locus tag: SA_RS01580 ]
• symbol: SA0269
• description: hypothetical protein
• length: 507
• theoretical pI: 8.34553
• theoretical MW: 57870
• GRAVY: -0.895858

⊟Function

• TIGRFAM:
• TheSEED  :
  • FIG01108182: hypothetical protein
• ⊞PFAM:
  no clan defined DUF6787; Family of unknown function (DUF6787) (PF20584; HMM-score: 14.6)
  BRCT-like (CL0459) PTCB-BRCT; twin BRCT domain (PF12738; HMM-score: 14)
  PBP (CL0177) PDT; Prephenate dehydratase (PF00800; HMM-score: 13.8)
  no clan defined Orthopox_F14; Orthopoxvirus F14 protein (PF06076; HMM-score: 12.7)
  and 3 more
  SapC; SapC (PF07277; HMM-score: 11.4)
  BRCT-like (CL0459) BRCT; BRCA1 C Terminus (BRCT) domain (PF00533; HMM-score: 11.3)
  no clan defined DUF378; Domain of unknown function (DUF378) (PF04070; HMM-score: 9.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helix: 1
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.8201
  • Cell wall & surface Score: 0.0167
  • Extracellular Score: 0.1632
• ⊞LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: Signal peptide SP(Sec/SPI) length 27 aa
  • SP(Sec/SPI): 0.703659
  • TAT(Tat/SPI): 0.002845
  • LIPO(Sec/SPII): 0.022967
  • Cleavage Site: CS pos: 27-28. VYA-FF. Pr: 0.4302
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15925982 NCBI
• RefSeq: NP_373515 NCBI
• UniProt: A0A0H3JKK7 UniProt

⊟Protein sequence

• MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQTAKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIKMSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLSTYSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNFDKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKDYDKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLEDDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLDNQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEPINFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0266 < SA0267 < SA0268 < SA0269

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications