From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0284 [new locus tag: SA_RS01655 ]
  • pan locus tag?: SAUPAN001211000
  • symbol: SA0284
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0284 [new locus tag: SA_RS01655 ]
  • symbol: SA0284
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 342089..342700
  • length: 612
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGAGTTCTTATTATTAATTGTCGTAGCCGGACTGTATTATATTATATATTTAACTGCT
    GTGATGTATTCTGAAAAAATAGTAGTATTGCCTATAATCATCTATGCCATTTTGTTTGTA
    ATAATTGGTATCACTTATATCTTTATAGGCGACAGCTATGATCAATTAACAAATTTCAAT
    GTGATTTTGTATATGGGGAGTTTGTTTTATGCATGGATGGCTATTAGAAATCTTTGGAAC
    AGACCACTATTACTAAAATATAAGAACATTACAGATAGTTCAAGTGGAATAGTTAATAAA
    TCTGAATACAATTCAGTTGAAAGCTTACGTATAAATATTGAAATAGCTAAGTATAAAGGG
    ATTATTTCTTTGATAGTAGCTATAGTACTAACGGTATTAATGACATTAAAGTCAACACCT
    CAAATCACTGCAGAAACACGTGACTTAAGTATCTCATTTTTCATACTCAGCTTATTTATC
    ATTATTATATTTGCAGTTTGGGATTTGTTTATTAGAGTTAGAAAAGGAGCGTTTGCTTTT
    GTTGTAATAAGGCCAATATTATTCAGTTGTTGGATATTTATTTTGAATATGATTTTATCA
    AGATTATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    612

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0284 [new locus tag: SA_RS01655 ]
  • symbol: SA0284
  • description: hypothetical protein
  • length: 203
  • theoretical pI: 9.5678
  • theoretical MW: 23394.2
  • GRAVY: 1.09015

Function[edit | edit source]

  • TIGRFAM:
    TIGR04222 domain (TIGR04222; HMM-score: 3.9)
  • TheSEED  :
    • FIG01108223: hypothetical protein
  • PFAM:
    no clan defined DUF5080; Domain of unknown function (DUF5080) (PF16883; HMM-score: 339.2)
    and 2 more
    Pox_A14; Poxvirus virion envelope protein A14 (PF05767; HMM-score: 15.9)
    Protocadherin; Protocadherin (PF08374; HMM-score: 11.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001677
    • TAT(Tat/SPI): 0.000096
    • LIPO(Sec/SPII): 0.007759
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAILFVIIGITYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSEYNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFIIIIFAVWDLFIRVRKGAFAFVVIRPILFSCWIFILNMILSRLL

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]